MicroRNAs （miRNAs） represent a class of small non-coding RNA molecules that play a crucial role in post- transcriptional gene regulation. Several conserved and species-specific miRNAs have been characterized to date...MicroRNAs （miRNAs） represent a class of small non-coding RNA molecules that play a crucial role in post- transcriptional gene regulation. Several conserved and species-specific miRNAs have been characterized to date, predominantly from the plant species whose genome is well characterized. However, information on the variability of these regulatory RNAs in economically important but genetically less characterized crop species are limited. Vigna mungo is an important grain legume, which is grown primarily for its protein-rich edible seeds, miRNAs from this species have not been identified to date due to lack of genome sequence information. To identify miRNAs from V. mungo, a small RNA library was constructed from young leaves. High- throughput IIlumina sequencing technology and bioinformat- ic analysis of the small RNA reads led to the identification of 66 miRNA loci represented by 45 conserved miRNAs belonging to 19 families and eight non-conserved miRNAs belonging to seven families. Besides, 13 novel miRNA candidates in V. mungo were also identified. Expressior patterns of selected conserved, non-conserved, and nove miRNA candidates have been demonstrated in leaf, stem, ant root tissues by quantitative polymerase chain reaction, ant potential target genes were predicted for most of the conserved miRNAs. This information offers genomic resour ces for better understanding of miRNA mediated post transcriptional gene regulation.展开更多
基金supported by the Council of Scientific and Industrial Research,New-Delhi,India for the Emeritus Scientist’s Scheme to AP (Sanction No. 21(0884)/12/EMR-II)by the Department of Biotechnology,India (Sanction no. BT/01/COE/06/03)
文摘MicroRNAs （miRNAs） represent a class of small non-coding RNA molecules that play a crucial role in post- transcriptional gene regulation. Several conserved and species-specific miRNAs have been characterized to date, predominantly from the plant species whose genome is well characterized. However, information on the variability of these regulatory RNAs in economically important but genetically less characterized crop species are limited. Vigna mungo is an important grain legume, which is grown primarily for its protein-rich edible seeds, miRNAs from this species have not been identified to date due to lack of genome sequence information. To identify miRNAs from V. mungo, a small RNA library was constructed from young leaves. High- throughput IIlumina sequencing technology and bioinformat- ic analysis of the small RNA reads led to the identification of 66 miRNA loci represented by 45 conserved miRNAs belonging to 19 families and eight non-conserved miRNAs belonging to seven families. Besides, 13 novel miRNA candidates in V. mungo were also identified. Expressior patterns of selected conserved, non-conserved, and nove miRNA candidates have been demonstrated in leaf, stem, ant root tissues by quantitative polymerase chain reaction, ant potential target genes were predicted for most of the conserved miRNAs. This information offers genomic resour ces for better understanding of miRNA mediated post transcriptional gene regulation.
