Carboxymethytl pachymaram(CMP)was administered to HBV transgenic mice through abdominal injection.Lymphocytes were extracted from the spleens.MTT method was used to detect cytotoxicity of CMP.Dendritic cells(DCs)were ...Carboxymethytl pachymaram(CMP)was administered to HBV transgenic mice through abdominal injection.Lymphocytes were extracted from the spleens.MTT method was used to detect cytotoxicity of CMP.Dendritic cells(DCs)were separated from lymphocytes and incubated with granulocyte-macrophage colony-stimulating factor(GM-CSF)and interleukin-4(IL-4).Phenotypes of DC’s were assayed by flow cytometry(FCM).IL-12 released by DCs and IL-10 and IFN-γ produced by T cells in mixed lymphocyte reaction(MLR)were measured using ELISA.Results showed that CMP within the concentration of 0–500 mg/mL did not produce cytotoxicity to lymphocytes and could even increase DC phenotypes,and IL-12 level in HBV transgenic mice.It could also increase the secretion of IFN-c,and inhibit the secretion of IL-10 inMLR.Thus it can up-regulateDC function.展开更多
基金This study was supported by the Science Foundation of Hubei Health Department(No.301140391).
文摘Carboxymethytl pachymaram(CMP)was administered to HBV transgenic mice through abdominal injection.Lymphocytes were extracted from the spleens.MTT method was used to detect cytotoxicity of CMP.Dendritic cells(DCs)were separated from lymphocytes and incubated with granulocyte-macrophage colony-stimulating factor(GM-CSF)and interleukin-4(IL-4).Phenotypes of DC’s were assayed by flow cytometry(FCM).IL-12 released by DCs and IL-10 and IFN-γ produced by T cells in mixed lymphocyte reaction(MLR)were measured using ELISA.Results showed that CMP within the concentration of 0–500 mg/mL did not produce cytotoxicity to lymphocytes and could even increase DC phenotypes,and IL-12 level in HBV transgenic mice.It could also increase the secretion of IFN-c,and inhibit the secretion of IL-10 inMLR.Thus it can up-regulateDC function.
