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Effects of Urtica dioica extract on CA3 hippocampal pyramidal cell loss in young diabetic rats
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作者 Seyyed Amirhossein Fazeli anneh mohammad gharravi +2 位作者 Soraya Ghafari Mehrdad Jahanshahi mohammad Jafar Golalipour 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第12期901-905,共5页
BACKGROUND: Urtica dioica extract has been shown to play a protective role in the neurodegeneration associated with diabetes mellitus. OBJECTIVE: To verify the neuroprotective efficacy of nettle extract on pyramidal... BACKGROUND: Urtica dioica extract has been shown to play a protective role in the neurodegeneration associated with diabetes mellitus. OBJECTIVE: To verify the neuroprotective efficacy of nettle extract on pyramidal cell density in the CA3 hippocampal subfield following administration of Urtica dioica extract to young diabetic rats. DESIGN, TIME AND SETTING: A randomized, controlled, neurobiological study was performed at the Department of Histology and Embryology at the Gorgan University of Medical Sciences in Iran from 2006 to 2007. MATERIALS: Urtica dioica leaves were collected from a cultivated plant in the suburb of Gorgan (northem Iran) and taxonomically identified by the Department of Pharmacognosy, Mazandaran University of Medical Sciences. METHODS: A total of 20 male, albino, Wistar rats, aged 6-7 postnatal weeks, were randomly assigned to four groups: normal control, diabetic model, preventive, and treatment, with five rats in each group. Diabetes was induced by intraperitoneal injection of streptozotocin (80 mg/kg) in the diabetic and treatment groups. Rats from the preventive group received a hydroalcoholic extract of Urtica dioica (100 mg/kg per day) during the first 5 days, and then streptozotocin (80 mg/kg) was administered on day 6. One week following the streptozotocin injection, rats in the treatment group were intraperitoneally administered hydroalcoholic extract of Urtica dioica (100 mg/kg per day) for 4 weeks. MAIN OUTCOME MEASURES: Following administration of Urtica dioica extract, the dorsal hippocampal formation of the right cerebral hemispheres was stained with cresyl violet. Area densities of CA3 pyramidal cells were measured. RESULTS: The diabetic, preventive, and treatment groups exhibited reduced cell densities compared with the control group (P 〈 0.05). Moreover, densities of CA3 pyramidal cells in the treatment group were significantly reduced compared with the diabetic model group (P 〈 0.05). CONCLUSION: The Urtica dioica extract exhibited no significant neuroprotectJve benefits in diabetes-induced loss of pyramidal cells in the CA3 hippocampal subfields of young diabetic rats. 展开更多
关键词 Urtica dioica HIPPOCAMPUS pyramidal cells diabetes neuroprotective efficacy
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Status of tissue engineering and regenerative medicine in Iran and related advanced tools: Bioreactors and scaffolds
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作者 anneh mohammad gharravi Mahmoud Orazizadeh +4 位作者 Mahmoud Hashemitabar Karim Ansari-Asl Salem Banoni Ali Alifard Sina Izadi 《Journal of Biomedical Science and Engineering》 2012年第4期217-227,共11页
Because of increased need to tissue and organ transplantation, tissue engineering (TE) researches have significantly increased in recent years in Iran. The present study explored briefly the advances in the TE approac... Because of increased need to tissue and organ transplantation, tissue engineering (TE) researches have significantly increased in recent years in Iran. The present study explored briefly the advances in the TE approaches in Iran. Through comprehensive search, we explored main TE components researches include cell, scaffold, growth factor and bioreactor conducted in Iran. The field of TE and regenerative medicine in Iran dates back to the early part of the 1990 decade and the advent of stem cell researches. During past two decades, Iran was one of leader in stem cell research in Middle East. The next major step in TE was application and fabrication of scaffolds for TE in the early 2000s with focused on engineering bone and nerve tissue. Iranian researchers extensively used natural scaffolds in their studies and hybridized natural polymers and inorganic scaffolds. There are many universities and government research institutes are conducting active research on tissue-engineering technologies. Limitations to TE in Iran include property design and validation of bioreactors. In conclusion, in the last few years, fields of tissue engineering and regenerative medicine such as stem cell technology and scaffolds have progressed in Iran, but one of the biggest challenges for TE is bioreactors researches. 展开更多
关键词 Iran TISSUE Engineering Cell SCAFFOLD Signal BIOREACTOR
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Mixed enzymatic-explant protocol for isolation of mesenchymal stem cells from Wharton’s jelly and encapsulation in 3D culture system
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作者 Saeed Azandeh Mahmoud Orazizadeh +5 位作者 Mahmoud Hashemitabar Ali Khodadadi Ali Akbar Shayesteh Darioush Bijan Nejad anneh mohammad gharravi Elham Allahbakhshi 《Journal of Biomedical Science and Engineering》 2012年第10期580-586,共7页
We report combination of explants and enzymatic protocol as mixed enzymatic-explant procedure to faster extraction of MSCs from WJ. Umbilical cords (UC) were collected from Imam Khomini Hospital. For explant outgrowth... We report combination of explants and enzymatic protocol as mixed enzymatic-explant procedure to faster extraction of MSCs from WJ. Umbilical cords (UC) were collected from Imam Khomini Hospital. For explant outgrowth, 6 - 9 pieces of WJ were transferred onto tissue culture flask and waited for attachment. For mixed enzymatic-explant, 1 cm3 pieces WJ were placed in enzymatic cocktail comprising 4 mg/ml Collagenase Type I and 1 mg/ml Hyaluronidase and 0.1% trypsin-EDTA. Then isolated cells were analyzed for surface cell markers such as CD73, CD31. Isolated 1.0 × 106 MSCs/ml were encapsulated in alginate hydrogel. Cells with MSCs phenotype were isolated from mixed enzymatic-explant and explant procedures within 24 - 48 hrs and 7 - 10 days, respectively. Both of procedures were shown to form clumps and colonies with dense centers. Phenotypic changes gradually appeared as round cell in UC pieces into homogeneous spindle-shaped and typical fibroblast-like shape cells. By using flow cytometery MSCs showed positive for CD73, and negative for CD31. the morphology of viable MSCs in the beads did not significantly show a different morphology pattern before and after the bead formation process. These findings are indicated that when mixed enzymatic-explant procedure is performed MSCs can be isolated faster and much higher from WJ. These finding is important in comparing with time consuming explants culture for isolation of MSCs. 展开更多
关键词 MESENCHYMAL Stem Cells ISOLATION Flow Cytometery ALGINATE
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