AIM:To investigate the anti-inflammatory effects of the sesquiterpenesα-humulene andβ-caryophyllene on pterygium fibroblasts.METHODS:Primary cultures of pterygium fibroblasts were established.Third passage pterygium...AIM:To investigate the anti-inflammatory effects of the sesquiterpenesα-humulene andβ-caryophyllene on pterygium fibroblasts.METHODS:Primary cultures of pterygium fibroblasts were established.Third passage pterygium fibroblasts were exposed toα-humulene andβ-caryophyllene separately and together.The cell viability was assessed by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide(MTT)assay at 12,24,48,and 72h after exposure.The levels of the inflammatory cytokines interleukin(IL)-1β,IL-6,IL-8,tumor necrosis factor(TNF)-αand IL-10 in the conditioned culture medium were assessed by enzymelinked immunosorbent assay(ELISA)at 12,24 and 48h after exposure.Data were statistically analyzed using Friedman repeated measures analysis of variances on ranks.RESULTS:The 25μmol/Lβ-caryophyllene induced significant decrease in the IL-6 production by pterygium fibroblasts 48h after the exposure(P=0.041).The levels of IL-1β,IL-8,IL-10,and TNF-αwere very low and had no statistically significant variations af ter exposure toα-humulene,β-caryophyllene,or both compounds together.CONCLUSION:The exposure to 25μmol/L ofβ-caryophyllene significantly reduce the production of IL-6 by pterygium fibroblasts after 48h.This sesquiterpene may be a potential alternative adjuvant agent for the treatment of pterygium.展开更多
基金Supported by Sao Paulo State Research Foundation(Fundacao de AmparoàPesquisa do Estado de S?o Paulo–FAPESPNo.2012/10032-4)supported by a fellowship by Coordination for the Improvement of Higher Level Education Personnel(CAPES),Ministry of Education,Brazil(No.06460/2013)。
文摘AIM:To investigate the anti-inflammatory effects of the sesquiterpenesα-humulene andβ-caryophyllene on pterygium fibroblasts.METHODS:Primary cultures of pterygium fibroblasts were established.Third passage pterygium fibroblasts were exposed toα-humulene andβ-caryophyllene separately and together.The cell viability was assessed by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide(MTT)assay at 12,24,48,and 72h after exposure.The levels of the inflammatory cytokines interleukin(IL)-1β,IL-6,IL-8,tumor necrosis factor(TNF)-αand IL-10 in the conditioned culture medium were assessed by enzymelinked immunosorbent assay(ELISA)at 12,24 and 48h after exposure.Data were statistically analyzed using Friedman repeated measures analysis of variances on ranks.RESULTS:The 25μmol/Lβ-caryophyllene induced significant decrease in the IL-6 production by pterygium fibroblasts 48h after the exposure(P=0.041).The levels of IL-1β,IL-8,IL-10,and TNF-αwere very low and had no statistically significant variations af ter exposure toα-humulene,β-caryophyllene,or both compounds together.CONCLUSION:The exposure to 25μmol/L ofβ-caryophyllene significantly reduce the production of IL-6 by pterygium fibroblasts after 48h.This sesquiterpene may be a potential alternative adjuvant agent for the treatment of pterygium.
