Anthracnose fruit rot(AFR),caused by the fungal pathogen Colletotrichum fioriniae,is among the most destructive and widespread fruit disease of blueberry,impacting both yield and overall fruit quality.Blueberry cultiv...Anthracnose fruit rot(AFR),caused by the fungal pathogen Colletotrichum fioriniae,is among the most destructive and widespread fruit disease of blueberry,impacting both yield and overall fruit quality.Blueberry cultivars have highly variable resistance against AFR.To date,this pathogen is largely controlled by applying various fungicides;thus,a more cost-effective and environmentally conscious solution for AFR is needed.Here we report three quantitative trait loci associated with AFR resistance in northern highbush blueberry(Vaccinium corymbosum).Candidate genes within these genomic regions are associated with the biosynthesis of flavonoids(e.g.anthocyanins)and resistance against pathogens.Furthermore,we examined gene expression changes in fruits following inoculation with Colletotrichum in a resistant cultivar,which revealed an enrichment of significantly differentially expressed genes associated with certain specialized metabolic pathways(e.g.flavonol biosynthesis)and pathogen resistance.Using non-targeted metabolite profiling,we identified a flavonol glycoside with properties consistent with a quercetin rhamnoside as a compound exhibiting significant abundance differences among the most resistant and susceptible individuals from the genetic mapping population.Further analysis revealed that this compound exhibits significant abundance differences among the most resistant and susceptible individuals when analyzed as two groups.However,individuals within each group displayed considerable overlapping variation in this compound,suggesting that its abundance may only be partially associated with resistance against C.fioriniae.These findings should serve as a powerful resource that will enable breeding programs to more easily develop new cultivars with superior resistance to AFR and as the basis of future research studies.展开更多
基金This work was supported by United States Department of Agriculture-Agriculture and Food Research Initiative(USDA-AFRI)#2018-67013-27592 to G.S.,T.M.and P.P.E.United States Department of Agriculture-Specialty Crop Research Initiative(USDASCRI)#2019-51181-30015 to P.P.E.+2 种基金National Institute of Health(NIH)Training Grant#T32-GM110523National Science Foundation(NSF)Training Grant DGE-1828149 to M.J.and P.P.EThis work was also supported by Michigan State University AgBioRe search and United States Department of Agriculture-HATCH MICL02742 to P.P.E.
文摘Anthracnose fruit rot(AFR),caused by the fungal pathogen Colletotrichum fioriniae,is among the most destructive and widespread fruit disease of blueberry,impacting both yield and overall fruit quality.Blueberry cultivars have highly variable resistance against AFR.To date,this pathogen is largely controlled by applying various fungicides;thus,a more cost-effective and environmentally conscious solution for AFR is needed.Here we report three quantitative trait loci associated with AFR resistance in northern highbush blueberry(Vaccinium corymbosum).Candidate genes within these genomic regions are associated with the biosynthesis of flavonoids(e.g.anthocyanins)and resistance against pathogens.Furthermore,we examined gene expression changes in fruits following inoculation with Colletotrichum in a resistant cultivar,which revealed an enrichment of significantly differentially expressed genes associated with certain specialized metabolic pathways(e.g.flavonol biosynthesis)and pathogen resistance.Using non-targeted metabolite profiling,we identified a flavonol glycoside with properties consistent with a quercetin rhamnoside as a compound exhibiting significant abundance differences among the most resistant and susceptible individuals from the genetic mapping population.Further analysis revealed that this compound exhibits significant abundance differences among the most resistant and susceptible individuals when analyzed as two groups.However,individuals within each group displayed considerable overlapping variation in this compound,suggesting that its abundance may only be partially associated with resistance against C.fioriniae.These findings should serve as a powerful resource that will enable breeding programs to more easily develop new cultivars with superior resistance to AFR and as the basis of future research studies.
