This study reports the frequency of decay(stem/butt rot)of Xylocarpus granatum trees at Hat Khanom-Mu Ko Thale Tai National Park,Nakhonsithammarat province,and other locations in Thailand,and the identification of pur...This study reports the frequency of decay(stem/butt rot)of Xylocarpus granatum trees at Hat Khanom-Mu Ko Thale Tai National Park,Nakhonsithammarat province,and other locations in Thailand,and the identification of purported causal basidiomycetes based on morphological and molecular analyses.Four survey plots at Hat KhanomMu Ko Thale Tai National Park were established and the incidence of butt rot determined.Percentage stem/butt rot incidence of X.granatum trees varied from 40.9 to 94.4%with an average of 85.5%along all four Plots.Trees in Plot 2 supported the heaviest incidence rate(94.4%),with the lowest rate in Plot 3(40.9%).Ninety-two basidiomes were collected,and 46 fungal strains(50%)isolated into axenic culture,the majority associated with tree roots(68.5%of all collections)with 31.5%from the tree trunks.Molecular results,based on LSU and ITS1,2,5.8 S rDNA analyses,confirmed that all samples belonged to the poroid genus Fulvifomes in the Hymenochaetaceae within the Hymenochaetales,Basidiomycota.The 43 specimens sequenced grouped into three clades;one clade comprised specimens isolated from only the trunks and branches(14 strains),while the remainders were from roots(29 strains)and shown to be salt tolerant.The stem/butt rot strains formed unique phylotypes which did not group with other known Fulvifomes species.展开更多
基金supported by the TRF/BIOTEC Special Program for Biodiversity Research and Training and Petroleum Authority of Thailand(PTT)research grant BRT R_252121 and the 2011 MAB Young Scientists Award,UNESCO.
文摘This study reports the frequency of decay(stem/butt rot)of Xylocarpus granatum trees at Hat Khanom-Mu Ko Thale Tai National Park,Nakhonsithammarat province,and other locations in Thailand,and the identification of purported causal basidiomycetes based on morphological and molecular analyses.Four survey plots at Hat KhanomMu Ko Thale Tai National Park were established and the incidence of butt rot determined.Percentage stem/butt rot incidence of X.granatum trees varied from 40.9 to 94.4%with an average of 85.5%along all four Plots.Trees in Plot 2 supported the heaviest incidence rate(94.4%),with the lowest rate in Plot 3(40.9%).Ninety-two basidiomes were collected,and 46 fungal strains(50%)isolated into axenic culture,the majority associated with tree roots(68.5%of all collections)with 31.5%from the tree trunks.Molecular results,based on LSU and ITS1,2,5.8 S rDNA analyses,confirmed that all samples belonged to the poroid genus Fulvifomes in the Hymenochaetaceae within the Hymenochaetales,Basidiomycota.The 43 specimens sequenced grouped into three clades;one clade comprised specimens isolated from only the trunks and branches(14 strains),while the remainders were from roots(29 strains)and shown to be salt tolerant.The stem/butt rot strains formed unique phylotypes which did not group with other known Fulvifomes species.
