HIV-1 infection requires the expression of CD4＋ molecules in colligation with C-C chemokine receptor type 5 （CCR5） and C-X-C chemokine receptor type 4 （CXCR4） as the major coreceptors. The role of SNP in 3＇ untr...HIV-1 infection requires the expression of CD4＋ molecules in colligation with C-C chemokine receptor type 5 （CCR5） and C-X-C chemokine receptor type 4 （CXCR4） as the major coreceptors. The role of SNP in 3＇ untranslated region ofSDF-1 （SDF1-3 ＇A） and low copy number （CN） of the CCL3L1 gene is reported to confer increased resistance to HIV-1 infection. The aim of the present study was to analyze the combinatorial effect of both the variations in protection towards HIV-1 infection in Indian population. The combinatorial effect of genetic variation in terms of SNP in SDF-1 gene and CCL3L1 CN was investigated in 105 healthy individuals and 78 HIV-I patients. Genotyping of SDF-1 was performed by RFLP-PCR and CCL3L1 by real-time PCR using TaqMan chemistry. The genotype frequency distribution of SDF-1 was found to be （SDF-1/SDF-I： 65.4%, SDF-1/SDF1-3＇A： 29.5% and SDFI-3＇A/SDF1-3＇A- 5.1%） in HIV patients as compared to （SDF-1/SDF-I： 64.8%, SDF-1/SDF1-3＇A： 30.5% and SDF1-3 ＇A/SDF1-3 ＇A： 4.7%） in healthy individuals, whereas a range of 1 to 6 copies per diploid genome was observed for CCL3L1 gene.展开更多
文摘HIV-1 infection requires the expression of CD4＋ molecules in colligation with C-C chemokine receptor type 5 （CCR5） and C-X-C chemokine receptor type 4 （CXCR4） as the major coreceptors. The role of SNP in 3＇ untranslated region ofSDF-1 （SDF1-3 ＇A） and low copy number （CN） of the CCL3L1 gene is reported to confer increased resistance to HIV-1 infection. The aim of the present study was to analyze the combinatorial effect of both the variations in protection towards HIV-1 infection in Indian population. The combinatorial effect of genetic variation in terms of SNP in SDF-1 gene and CCL3L1 CN was investigated in 105 healthy individuals and 78 HIV-I patients. Genotyping of SDF-1 was performed by RFLP-PCR and CCL3L1 by real-time PCR using TaqMan chemistry. The genotype frequency distribution of SDF-1 was found to be （SDF-1/SDF-I： 65.4%, SDF-1/SDF1-3＇A： 29.5% and SDFI-3＇A/SDF1-3＇A- 5.1%） in HIV patients as compared to （SDF-1/SDF-I： 64.8%, SDF-1/SDF1-3＇A： 30.5% and SDF1-3 ＇A/SDF1-3 ＇A： 4.7%） in healthy individuals, whereas a range of 1 to 6 copies per diploid genome was observed for CCL3L1 gene.
