Quantitative analysis of γ-oryzanol content in cold pressed rice bran oil by TLC-image analysis method

Objective:To develop and validate an image analysis method for quantitative analysis ofγ-oryzanol in cold pressed rice bran oil.Methods:TLC-densitometric and TLC-image analysis methods were developed,validated,and used for quantitative analysis of γ-oryzanol in cold pressed rice bran oil.The results obtained by these two different quantification methods were compared by paired t-test.Results:Both assays provided good linearity,accuracy,reproducibility and selectivity for determination of γ-oryzanol.Conclusions:The TLC-densitomelric and TLC-image analysis methods providett a similar reproducibility,accuracy and selectivity for the quantitative determination of γ-oryzanol in cold pressed rice bran oil.A statistical comparison of the quantitative determinations of γ-oryzanol in samples did not show any statistically significant difference between TLC-densitometric and TLC-image analysis methods.As both methods were found to be equal,they therefore can be used for the determination of γ-oryzanol in cold pressed rice bran oil.

Zingiber cassumunar blended patches for skin application:Formulation,physicochemical properties,and in vitro studies

Our work was to study the preparation,physicochemical characterization,and in vitro characteristic of Zingiber cassumunar blended patches.The Z.cassumunar blended patches incorporating Z.cassumunar Roxb.also known as Plai were prepared from chitosan and polyvinyl alcohol with glycerin as plasticizer.They were prepared by adding all ingredients in a beaker and homogeneously mixing them.Then,they were transferred into Petri-dish and dried in hot air oven.The hydrophilic nature of the Z.cassumunar blended patches was confirmed by the moisture uptake,swelling ratio,erosion,and porosity values.The FTIR,DSC,XRD,and SEM studies showed revealed blended patches with amorphous region that was homogeneously smooth and compact in both surface and cross section dimensions.They exhibited controlled the release behavior of(E)-4-(30,40-dimethoxyphenyl)but-3-en-lol(compound D)that is the main active compound in Z.cassumunar for anti-inflammation activity.However,in in vitro skin permeation study,the compound D was accumulated in newborn pig skin more than in the receptor medium.Thus,the blended patches showed the suitable entrapment and controlled release of compound D.Accordingly,we have demonstrated that such chitosan and polyvinyl alcohol formulated patches might be developed for medical use.

基于液相色谱-质谱代谢组学方法辨别两种泰国籼稻冷榨米糠油（英文）

A newly developed liquid chromatography-mass spectrometry(LC-MS)method for the analysis of cold pressed rice bran oil(RBO)was established and used to discriminate between RBOs produced from two different cultivars of major Thai fragrant rice species.The cold pressed RBO was prepared using the screw compression method.The LC-MS data were preprocessed with MZmine 2.10 program before evaluating with principal component analysis using SIMCA 13 software.The LC-MS method was able to detect and quantify several kinds of valuable constituents such as fatty acids,vitamin E,andγ-oryzanol.The chromatographic condition was feasible;short time for analysis and simple method were achieved.From score plot and loading plot of principle component analysis(PCA),two rice cultivar samples were clearly separated,and it was revealed that Khao-Hom-Pathum was more suitable than Khao-Hom-Mali for cold pressed RBO production since it contained high totalγ-oryzanol and less saturated free fatty acids.As with the fixed price of all the rice brans,this information can be used in order to,if possible,preserve the price of rice brans from different cultivars.