Anti-oxidative role of quercetin derived from Allium cepa on aldehyde oxidase(OX-LDL)and hepatocytes apoptosis in streptozotocininduced diabetic rat

Objective:To study the role of Quercetin in streptozotocin-induced diabetes in rals.Methods:Wistar male rat(n=40) were allocated into three groups,control group(n=10) and Quercetin(QR) group received 15 mg/kg(IP) QR.(n=10),and diabetic group that received 55 mg/kg(IP)streptozotocin(STZ)(n=20) which was subdivided to two groups of 10;STZ group and treatment group.Treatment group received 55mg/kg(IP) STZ plus 15 nig/kg QR,daily lor 4 weeks,respectively:however,the control group just received an equal volume of distilled water dailv(IP).Diabetes was induced by a single(IP) injection of streptozotocin(55 mg/kg).Animal-were kept in standard condition.Twenty-eight days after inducing diabetic,5 mL blood were collected for TAC,MPA and Ox-LDL levels and liver tissues of rat in whole groups were removed then prepared for apoptosis analysis by Tunel method.Results:Apoptotic cells significantly decreased in group that has received 15 mg/kg(IP) Quercetin(P<0.05) in comparison to experimental groups(P<0.05).Conclusions:Since in our study 15 nig/kg(IP) Quercetin have significantly Preventive ellecl on liver cells damages by reducing number of apoptotic cells in Liver,so it seems that using it can be effective for treatment in diabetic rat.

Effects of Matricaria chamomilla Extract on Growth and Maturation of Isolated Mouse Ovarian Follicles in a Three-dimensional Culture System

Background： The aim of this study was to design and assess the effects of hydroalcoholic extract of Matricaria chamomill^l （MC） on preantral follicle culture of mouse ovaries in a three-dimensional culture system. Methods： Isolated preantral follicles were randomly divided into three main groups： the control group containing 10% fetal bovine serum without MC extract （G1）, the first experimental group supplemented with 25 ktg/ml hydroalcoholic extract of chamomile （G2）, and the second experimental group supplemented with 50 p,g/ml hydroalcoholic extract of chamomile （G3）. Results： After 12 days of culture, the survival rate （P 〈 0.05）, antrum formation （P 〈 0.01）, metaphase two oocytes （P 〈 0.01）, and the expression ofPCNA （P 〈 0.05） and FSHR （P 〈 0.05） genes significantly decreased in G3 as compared with GI. On the other hand, at the last day of culture （day 12）, the mean diameter of follicles cultured in the medium which was supplemented with 50 lag/ml hydroalcoholic extract of chamomile significantly decreased as compared with the GI （P 〈 0.05）. In addition, the levels of progesterone and dehydroepiandrosterone hormones significantly increased in the medium of G3 relative to G I （P 〈 0.01）, while in the medium of G 1, the level of 17[3-estradiol was significantly higher than that of other groups （P 〈 0.01）. Reactive oxygen species levels of metaphase 11 oocytes were significantly decreased in G2 as compared with G1 （P 〈 0.01）. Conclusion： Adding chamomile extract to culture media appeared to decrease follicular function and development.

Side Effects of Pyrethroid and Supporting Role of Onion in the Male Rat＇s Spermatogenesis

To the Editor：Permethrin is a great handmade of chemical agents,pyrethroid Type Ⅰ,which exists in two main isomers,Cis and Trans.[1] Its application in farming and building is due to well availability and repelling of pests.It may be easy absorbed from the gastrointestinal and skin tract and by inhalation of dust.[2,3] They are great chemicals with high producing of reactive oxygen species and inducer of necrosis pathway.