Lactic acid bacteria (LAB) were isolated from fresh sugar cane juice. It was found that several isolates exhibited a clear zone and growth on deMan, Rogosa, Sharpe (MRS) agar supplemented with sodium azide, bromocreso...Lactic acid bacteria (LAB) were isolated from fresh sugar cane juice. It was found that several isolates exhibited a clear zone and growth on deMan, Rogosa, Sharpe (MRS) agar supplemented with sodium azide, bromocresol purple and sucrose. However, only 17 isolates which formed large yellow areas were selected for further investigations. These isolates were further identified according to their morphological and biochemical characteristics. It was found that 10 of these isolates were homofermenters. One of these 10 isolates was selected for mutagenesis using chemical (Ethidium bromide) and physical (UV-B) mutagens followed by biochemical characterisation. A total of 112 mutants were isolated and 9 homofermentative isolates were further investigated for their ability to produce lactic acid. 1H-NMR spectroscopy confirmed that all mutant isolates produced lactic acid as the sole fermentation product.展开更多
Much before modern medicine started to explore the medical applications of materials,archeological findings show that biomaterials such as corals and shells were used for replacement of missing human bones and teeth a...Much before modern medicine started to explore the medical applications of materials,archeological findings show that biomaterials such as corals and shells were used for replacement of missing human bones and teeth achieving osseointegration.At that time,no boundaries segregated the multidisciplinary nature of science.In the 1900s,surgeons and dentists were the first to adopt the use of materials in surgery and implants without any real collaboration with scientists and engineers.After World War Ⅱ,the term biomaterials started to take shape with inherent interdisciplinary attributes unifying materials science,chemistry,biology,physics,engineering,biomechanics,and finally,nanotechnology.展开更多
文摘Lactic acid bacteria (LAB) were isolated from fresh sugar cane juice. It was found that several isolates exhibited a clear zone and growth on deMan, Rogosa, Sharpe (MRS) agar supplemented with sodium azide, bromocresol purple and sucrose. However, only 17 isolates which formed large yellow areas were selected for further investigations. These isolates were further identified according to their morphological and biochemical characteristics. It was found that 10 of these isolates were homofermenters. One of these 10 isolates was selected for mutagenesis using chemical (Ethidium bromide) and physical (UV-B) mutagens followed by biochemical characterisation. A total of 112 mutants were isolated and 9 homofermentative isolates were further investigated for their ability to produce lactic acid. 1H-NMR spectroscopy confirmed that all mutant isolates produced lactic acid as the sole fermentation product.
文摘Much before modern medicine started to explore the medical applications of materials,archeological findings show that biomaterials such as corals and shells were used for replacement of missing human bones and teeth achieving osseointegration.At that time,no boundaries segregated the multidisciplinary nature of science.In the 1900s,surgeons and dentists were the first to adopt the use of materials in surgery and implants without any real collaboration with scientists and engineers.After World War Ⅱ,the term biomaterials started to take shape with inherent interdisciplinary attributes unifying materials science,chemistry,biology,physics,engineering,biomechanics,and finally,nanotechnology.
