Objective:To compare Wuchereria bancrofti(W.bancrofti)infection rates of Culex quinquefasciatus,using dissection and PCR-EUSA in two consecutive time periods(from 2007to 2008 and from 2008 to 2009).Methods:Mosquitoes ...Objective:To compare Wuchereria bancrofti(W.bancrofti)infection rates of Culex quinquefasciatus,using dissection and PCR-EUSA in two consecutive time periods(from 2007to 2008 and from 2008 to 2009).Methods:Mosquitoes were collected in 30 sentinel and 15 nonsentinel sites in 15 Medical Officer of Health areas of Gampaha District known for the presence of W.bancrofti transmission in two consecutive time period of 2007 to 2008 and 2008 to 2009.Captured mosquitoes were dissected to determine the W.bancrofti larvae(L_1 L_2,L_3,).PCR was carried out using DNA extracted from mosquito pools(15 body parts/pool)utilizing the primers specific for Wb-Sspl repeat.PCR products were analyzed by hybridization ELISA using fluorescein-labeled wild type specific probes.The prevalence of infected/infective mosquiloes in PCR pools(3 pools/site)was estimated using the PoolSereen^(TM)algorithm and a novel probability—based method.Results:Of 45 batches of mosquitoes dissected,W.bancrofti infected mosquitoes were found in19 and 13 batches,with an infection rate of 13.29%and 3.10%with mean larval density of 8.7 and1.0 larvae per mosquito for two study periods in the Gampaha District.Total of 405 pools of head,thorax and abdomen were processed by PCR-EUSA for each year.Of these,51 and 31 pools were positive for W.bancrofti in the two study periods respectively.The association of dissection based prevalence rates with PCR based rates as determined by the Pearson correlation coefficient were0.176 and 0.890 respectively for the two periods.Conclusions:Data indicate that PCR-EUSA is more sensitive than the traditional dissection techniques for monitoring transmission intensity.展开更多
基金Supported by the WHO/SEARO/TDR(Grant No.SN 1152)University of Kelaniya(Research Grant No.RP/03/04/06/01/2006)
文摘Objective:To compare Wuchereria bancrofti(W.bancrofti)infection rates of Culex quinquefasciatus,using dissection and PCR-EUSA in two consecutive time periods(from 2007to 2008 and from 2008 to 2009).Methods:Mosquitoes were collected in 30 sentinel and 15 nonsentinel sites in 15 Medical Officer of Health areas of Gampaha District known for the presence of W.bancrofti transmission in two consecutive time period of 2007 to 2008 and 2008 to 2009.Captured mosquitoes were dissected to determine the W.bancrofti larvae(L_1 L_2,L_3,).PCR was carried out using DNA extracted from mosquito pools(15 body parts/pool)utilizing the primers specific for Wb-Sspl repeat.PCR products were analyzed by hybridization ELISA using fluorescein-labeled wild type specific probes.The prevalence of infected/infective mosquiloes in PCR pools(3 pools/site)was estimated using the PoolSereen^(TM)algorithm and a novel probability—based method.Results:Of 45 batches of mosquitoes dissected,W.bancrofti infected mosquitoes were found in19 and 13 batches,with an infection rate of 13.29%and 3.10%with mean larval density of 8.7 and1.0 larvae per mosquito for two study periods in the Gampaha District.Total of 405 pools of head,thorax and abdomen were processed by PCR-EUSA for each year.Of these,51 and 31 pools were positive for W.bancrofti in the two study periods respectively.The association of dissection based prevalence rates with PCR based rates as determined by the Pearson correlation coefficient were0.176 and 0.890 respectively for the two periods.Conclusions:Data indicate that PCR-EUSA is more sensitive than the traditional dissection techniques for monitoring transmission intensity.
