Detection of Babesia bovis in blood samples and its effect on the hematological and serum biochemical profile in large ruminants from Southern Punjab

Objective:To determine the presence of Babesia bovis(B.bovis) in large ruminants in southern Punjab and its effect on hematological and serum biochemical profile of host animals.Methods:Blood samples were collected from 144 large ruminants,including 105 cattle and 39 buffaloes,from six districts in southern Punjab including Multan,Layyah,Muzaffar Garh,Bhakar,Bahawalnagar and Vehari.Data on the characteristics of animals and herds were collected through questionnaires.Different blood(hemoglobin,glucose) and serum(ALT,AST,LDH,cholesterol)parameters of calves and cattle were measured and compared between parasite positive and negative samples to demonstrate the effect of B.bovis on the blood and serological profile of infected animals.Results:27 out of 144 animals.from 5 out of 6 sampling districts,produced the541-bp fragment specific for B.bovis.Age of animals(P=0.02).presence of ticks on animals(P=0.04)and presence of ticks on dogs associated with herds(P=0.5) were among the major risk factors involved in the spread of bovine babesiosis in the study area.ALT concentrations were the only serum biochemical values that significantly varied between parasite positive and negative cattle.Conclusions:This study has reported for the first time the presence of B.bovis in large ruminant and the results can lead to the prevention of babesiosis in the region to increase the livestock output.

Effect of age and gender on some blood biochemical parameters of apparently healthy small ruminants from Southern Punjab in Pakistan

Objective:To report and compare the hematological and serum biochemical profile of goal and sheep from Rahim Yar Khan district in Southern Punjab.Methods:One hundred and twenty blood samples(98 goat and 22 sheep) were collected and their blood glucose, hemoglobin and serum biochemical parameters,cholesterol,aspartate transaminase(AST), alanine aminotransferase(ALT) and lactate dehydrogenase(LDH) were determined by using spectrophotometer.Parameters were compared between goat and sheep.Results:It was observed that glucose(P=0.001),LDH(P=0.001) and ALT(P=0.001) concentrations differed significantly between sheep and goats.The studied parameters were compared between buck and goats and also between young goats(less than 12 months old) and adults(more than 12 months old).It was found that age and gender did not affect these hematological and serum biochemical parameters. Condusions:It can be concluded that sheep have overall higher values than goals for studied hematological and serum biochemical parameters.