Spinal cord injury（SCI）frequently results in the permanent loss of function below the level of injury due to the failure of axonal regeneration in the adult mammalian central nervous system（CNS）.The limited intrin...Spinal cord injury（SCI）frequently results in the permanent loss of function below the level of injury due to the failure of axonal regeneration in the adult mammalian central nervous system（CNS）.The limited intrinsic growth capacity of adult neurons,a lack of growth-promoting factors and the multifactorial inhibitory microenvironment around the lesion site contribute to the lack of axonalregeneration. Strategies such as transplantation of cells,展开更多
On April 26-27, 2013, the Step by Step Foundation hosted the Second International Spinal Cord Repair Meeting at the Fira Barcelona Convention Center in Hospitalet de Llobregat, Spain, highlighting some of the exciting...On April 26-27, 2013, the Step by Step Foundation hosted the Second International Spinal Cord Repair Meeting at the Fira Barcelona Convention Center in Hospitalet de Llobregat, Spain, highlighting some of the exciting research including clinical trials which show promise for treatments for this devastating disorder. This meeting brought together clinicians, clinical scientists and molecular biologists from more than 10 countries to evaluate current knowledge on clinical, cellular, and biomolecular aspects of spinal cord injury. A major goal of the conference in advancing the translation of research data to the clinic was to promote multi-pronged approaches for therapy of this complex problem.展开更多
Alginate capillary hydrogels seeded with differentiated cells can fill the lesion cavity and promote axonal regeneration after grafting into the injured spinal cord.Neural stem/progenitor cells(NSPCs)can potentially r...Alginate capillary hydrogels seeded with differentiated cells can fill the lesion cavity and promote axonal regeneration after grafting into the injured spinal cord.Neural stem/progenitor cells(NSPCs)can potentially repair the spinal cord;however,effects of alginate hydrogels(AHs)on NSPCs remain unknown.In this study,we fabricated AHs cross-linked by Ca^(2+)and seeded hydrogels with rat embryonic day 14 NSPCs.Immunocytochemistry and electron microscopy show that NSPCs survive,proliferate and differentiate into neurons in vitro within the capillaries.After transplantation into an acute T8 complete spinal cord transection site in adult rats,approximately one-third(38.3%)of grafted cells survive and differentiate into neurons(40.7%),astrocytes(26.6%)and oligodendrocytes(28.4%)at 8weeks post-grafting.NSPCs promote the growth of host axons within the capillaries in a time-dependent manner.Host axons make synapse-like contacts with NSPC-derived neurons within the hydrogel channels,and graft-derived axons extend into the host white and gray matter making putative synapses.This is paralleled by improved electrophysiological conductivity across the lesion and partial hindlimb locomotor recovery.展开更多
基金Supported by grants from the Deutsche Forschungsgemeinschaft(BL414/3-1)International Foundation for Research in Paraplegia+2 种基金the Indiana University Health-Indiana University School of Medicine Strategic Research InitiativeIndiana Spinal Cord and Brain Injury Research FundMorton Cure Paralysis Fund to AB and a Heinz Gotze Memorial Fellowship to SL
文摘Spinal cord injury（SCI）frequently results in the permanent loss of function below the level of injury due to the failure of axonal regeneration in the adult mammalian central nervous system（CNS）.The limited intrinsic growth capacity of adult neurons,a lack of growth-promoting factors and the multifactorial inhibitory microenvironment around the lesion site contribute to the lack of axonalregeneration. Strategies such as transplantation of cells,
文摘On April 26-27, 2013, the Step by Step Foundation hosted the Second International Spinal Cord Repair Meeting at the Fira Barcelona Convention Center in Hospitalet de Llobregat, Spain, highlighting some of the exciting research including clinical trials which show promise for treatments for this devastating disorder. This meeting brought together clinicians, clinical scientists and molecular biologists from more than 10 countries to evaluate current knowledge on clinical, cellular, and biomolecular aspects of spinal cord injury. A major goal of the conference in advancing the translation of research data to the clinic was to promote multi-pronged approaches for therapy of this complex problem.
基金supported by the National Natural Science Fund of China(grant number 81901895 to S.L.,grant number 81571242 to Y.W.).
文摘Alginate capillary hydrogels seeded with differentiated cells can fill the lesion cavity and promote axonal regeneration after grafting into the injured spinal cord.Neural stem/progenitor cells(NSPCs)can potentially repair the spinal cord;however,effects of alginate hydrogels(AHs)on NSPCs remain unknown.In this study,we fabricated AHs cross-linked by Ca^(2+)and seeded hydrogels with rat embryonic day 14 NSPCs.Immunocytochemistry and electron microscopy show that NSPCs survive,proliferate and differentiate into neurons in vitro within the capillaries.After transplantation into an acute T8 complete spinal cord transection site in adult rats,approximately one-third(38.3%)of grafted cells survive and differentiate into neurons(40.7%),astrocytes(26.6%)and oligodendrocytes(28.4%)at 8weeks post-grafting.NSPCs promote the growth of host axons within the capillaries in a time-dependent manner.Host axons make synapse-like contacts with NSPC-derived neurons within the hydrogel channels,and graft-derived axons extend into the host white and gray matter making putative synapses.This is paralleled by improved electrophysiological conductivity across the lesion and partial hindlimb locomotor recovery.
