Accumulation of recalcitrant plastics in the environment has become a world-wide problem in today’s societies. Rapid depletion of natural resources for synthetic plastics along with environmental concerns has directe...Accumulation of recalcitrant plastics in the environment has become a world-wide problem in today’s societies. Rapid depletion of natural resources for synthetic plastics along with environmental concerns has directed research towards finding alternatives to petroleum-based polymers. Poly(3-hydroxybutyric acid) P(3HB), as one of these alternatives, have attracted much attention in recent years due to their varied mechanical properties, biocompatibility and biodegradability. The aim of this study was to identify an agro-industrial waste resource economically suitable for large-scale production of P(3HB), to optimize the production using Response Surface Methodology in small-scale and subsequently, to test the production in a continuously stirred tank reactor. Among a range of agro-industrial waste, orange peel was selected as the most suitable for P(3HB) production. P(3HB) concentration of 1.24 g P(3HB)/L culture broth with 41% P(3HB)/dcw yield was obtained using orange peel as the sole carbon source in optimized medium with a modified strain of Bacillus subtilis (B. subtilis OK2).展开更多
Phenol, as a pure substance, is used in many fields due to its disinfectant, germicidal, local anaesthetic and peptizing properties. Aqueous solutions of phenol are produced as a waste of these industries and are disc...Phenol, as a pure substance, is used in many fields due to its disinfectant, germicidal, local anaesthetic and peptizing properties. Aqueous solutions of phenol are produced as a waste of these industries and are discharged into the environment. Therefore, elevated concentrations of phenol may be found in air or water due to industrial discharge or use of phenolic products. The aim of this study was to evaluate the phenol removal capability of enzymes from low-phenol-content (up to 5%) industrial wastewaters and to optimize the reaction conditions. For this purpose, two different enzymes namely, Laccase and Peroxidase were investigated with respect to their phenol removal capacities. The enzymatic reaction conditions were optimized using Response Surface Methodology (RSM). As a result 78% phenol removal was achieved with laccase using a model wastewater. In the studies where the enzyme was immobilized, a 50% removal was achieved indicating that further optimization was needed in this area.展开更多
文摘Accumulation of recalcitrant plastics in the environment has become a world-wide problem in today’s societies. Rapid depletion of natural resources for synthetic plastics along with environmental concerns has directed research towards finding alternatives to petroleum-based polymers. Poly(3-hydroxybutyric acid) P(3HB), as one of these alternatives, have attracted much attention in recent years due to their varied mechanical properties, biocompatibility and biodegradability. The aim of this study was to identify an agro-industrial waste resource economically suitable for large-scale production of P(3HB), to optimize the production using Response Surface Methodology in small-scale and subsequently, to test the production in a continuously stirred tank reactor. Among a range of agro-industrial waste, orange peel was selected as the most suitable for P(3HB) production. P(3HB) concentration of 1.24 g P(3HB)/L culture broth with 41% P(3HB)/dcw yield was obtained using orange peel as the sole carbon source in optimized medium with a modified strain of Bacillus subtilis (B. subtilis OK2).
文摘Phenol, as a pure substance, is used in many fields due to its disinfectant, germicidal, local anaesthetic and peptizing properties. Aqueous solutions of phenol are produced as a waste of these industries and are discharged into the environment. Therefore, elevated concentrations of phenol may be found in air or water due to industrial discharge or use of phenolic products. The aim of this study was to evaluate the phenol removal capability of enzymes from low-phenol-content (up to 5%) industrial wastewaters and to optimize the reaction conditions. For this purpose, two different enzymes namely, Laccase and Peroxidase were investigated with respect to their phenol removal capacities. The enzymatic reaction conditions were optimized using Response Surface Methodology (RSM). As a result 78% phenol removal was achieved with laccase using a model wastewater. In the studies where the enzyme was immobilized, a 50% removal was achieved indicating that further optimization was needed in this area.
