Thymoquinone(TQ), an active component derived from the medial plant Nigella sativa, has been used for medical purposes for more than 2 000 years. Recent studies have reported that TQ blocked angiogenesis in animal mod...Thymoquinone(TQ), an active component derived from the medial plant Nigella sativa, has been used for medical purposes for more than 2 000 years. Recent studies have reported that TQ blocked angiogenesis in animal model and reduced migration, adhesion, and invasion of glioblastoma cells. We have recently shown that TQ could exhibit a potent cytotoxic effect and induce apoptosis in mouse neuroblastoma(Neuro-2a) cells. In the present study, TQ treatment markedly decreased the adhesion and migration of Neuro-2a cells. TQ down-regulated MMP-2 and MMP-9 protein expression and m RNA levels and their activities. Furthermore, TQ significantly down-regulated the protein expression of transcription factor NF-κB(p65) but not significantly altered the expression of N-Myc. Taken together, our data indicated that TQ's inhibitory effect on the migration of Neuro-2a cells was mediated through the suppression of MMP-2 and MMP-9 expression, suggesting that TQ treatment can be a promising therapeutic strategy for human malignant neuroblastoma.展开更多
Chemical modifications play an important role in modifying and regulating the functions of coding and noncoding RNAs.Noncoding RNAs mainly include microRNAs(miRNAs),long noncoding RNAs(lncRNAs),and circular RNAs(circR...Chemical modifications play an important role in modifying and regulating the functions of coding and noncoding RNAs.Noncoding RNAs mainly include microRNAs(miRNAs),long noncoding RNAs(lncRNAs),and circular RNAs(circRNAs).A common feature of these noncoding RNAs is that they are all transcribed from the genome and can perform biological functions.Importantly,the stable expression of noncoding RNAs in vivo makes them potential biomarkers for the diagnosis,prognosis,and clinical treatment of cancers and a variety of immunological diseases.展开更多
N6-Methyladenosine(m6A)is the most common and abundant modification of RNA molecules,including mRNAs,microRNAs(miRNAs),and long noncoding RNAs(lncRNAs).1 The m6A modification is catalyzed and dynamically regulated by ...N6-Methyladenosine(m6A)is the most common and abundant modification of RNA molecules,including mRNAs,microRNAs(miRNAs),and long noncoding RNAs(lncRNAs).1 The m6A modification is catalyzed and dynamically regulated by recently discovered enzymes termed“writers”(methyltransferases including METTL3,METTL14,and WTAP),“erasers”(demethylases including FTO and ALKBH5)and“readers”(m6A-binding proteins including YTHDC1,YTHDC2,YTHDF1,YTHDF2,and YTHDF3).These modifications were recently shown to be involved in the regulation of different biological processes.展开更多
文摘Thymoquinone(TQ), an active component derived from the medial plant Nigella sativa, has been used for medical purposes for more than 2 000 years. Recent studies have reported that TQ blocked angiogenesis in animal model and reduced migration, adhesion, and invasion of glioblastoma cells. We have recently shown that TQ could exhibit a potent cytotoxic effect and induce apoptosis in mouse neuroblastoma(Neuro-2a) cells. In the present study, TQ treatment markedly decreased the adhesion and migration of Neuro-2a cells. TQ down-regulated MMP-2 and MMP-9 protein expression and m RNA levels and their activities. Furthermore, TQ significantly down-regulated the protein expression of transcription factor NF-κB(p65) but not significantly altered the expression of N-Myc. Taken together, our data indicated that TQ's inhibitory effect on the migration of Neuro-2a cells was mediated through the suppression of MMP-2 and MMP-9 expression, suggesting that TQ treatment can be a promising therapeutic strategy for human malignant neuroblastoma.
文摘Chemical modifications play an important role in modifying and regulating the functions of coding and noncoding RNAs.Noncoding RNAs mainly include microRNAs(miRNAs),long noncoding RNAs(lncRNAs),and circular RNAs(circRNAs).A common feature of these noncoding RNAs is that they are all transcribed from the genome and can perform biological functions.Importantly,the stable expression of noncoding RNAs in vivo makes them potential biomarkers for the diagnosis,prognosis,and clinical treatment of cancers and a variety of immunological diseases.
文摘N6-Methyladenosine(m6A)is the most common and abundant modification of RNA molecules,including mRNAs,microRNAs(miRNAs),and long noncoding RNAs(lncRNAs).1 The m6A modification is catalyzed and dynamically regulated by recently discovered enzymes termed“writers”(methyltransferases including METTL3,METTL14,and WTAP),“erasers”(demethylases including FTO and ALKBH5)and“readers”(m6A-binding proteins including YTHDC1,YTHDC2,YTHDF1,YTHDF2,and YTHDF3).These modifications were recently shown to be involved in the regulation of different biological processes.
