Aim:A significant fraction of mortalities from non-small cell lung cancer could be prevented,if the cancer would be diagnosed earlier.Nanobiosensors for the ultrasensitive detection of active proteases in serum were d...Aim:A significant fraction of mortalities from non-small cell lung cancer could be prevented,if the cancer would be diagnosed earlier.Nanobiosensors for the ultrasensitive detection of active proteases in serum were designed to detect a significant protease activity signature of non-small cell lung cancer(stage I and higher).Methods:We determined the activity of nine protease biomarkers in the sera of non-small cell lung cancer patients and compared them with the protease activities of a control group of healthy human subjects using optical nanobiosensors.They consist of a central Fe/Fe3O4 core/shell nanoparticle with an attached Fluorescence resonance energy transfer-pair[tetrakis-carboxyphenyl porphyrin(TCPP)and cyanine 5.5].TCPP is attached to the central nanoparticle via a protease-cleavable tether,whereas cyanine 5.5 is tethered permanently to the dopamine-layer surrounding the nanoparticle.Results:Based on the activity pattern of urokinase plasminogen activator,matrix metalloproteinases 1,2,3,7,9,and 13,and cathepsins B and L as well,non-small cell lung cancer could be detected at stage I by means of a liquid biopsy.Conclusion:This feasibility study,comprising 33 non-small cell lung cancer patients and 20 apparently healthy subjects,clearly demonstrated the feasibility of minimally invasive early diagnosis of non-small cell lung cancer,starting with stage I.展开更多
文摘Aim:A significant fraction of mortalities from non-small cell lung cancer could be prevented,if the cancer would be diagnosed earlier.Nanobiosensors for the ultrasensitive detection of active proteases in serum were designed to detect a significant protease activity signature of non-small cell lung cancer(stage I and higher).Methods:We determined the activity of nine protease biomarkers in the sera of non-small cell lung cancer patients and compared them with the protease activities of a control group of healthy human subjects using optical nanobiosensors.They consist of a central Fe/Fe3O4 core/shell nanoparticle with an attached Fluorescence resonance energy transfer-pair[tetrakis-carboxyphenyl porphyrin(TCPP)and cyanine 5.5].TCPP is attached to the central nanoparticle via a protease-cleavable tether,whereas cyanine 5.5 is tethered permanently to the dopamine-layer surrounding the nanoparticle.Results:Based on the activity pattern of urokinase plasminogen activator,matrix metalloproteinases 1,2,3,7,9,and 13,and cathepsins B and L as well,non-small cell lung cancer could be detected at stage I by means of a liquid biopsy.Conclusion:This feasibility study,comprising 33 non-small cell lung cancer patients and 20 apparently healthy subjects,clearly demonstrated the feasibility of minimally invasive early diagnosis of non-small cell lung cancer,starting with stage I.
