Stable state of serum inflammatory cytokines during induction of benign prostate hyperplasia in dogs

Objective:To monitor serum inflammatory cytokines during induction of benign prostate hyperplasia in dogs.Methods:This research was designed as a case-control study.There were 20 adult mixed-breed intact male dogs,which were divided into the normal group(n=10)and the benign prostate hyperplasia group(n=10).In the benign prostate hyperplasia group,benign prostate hyperplasia was induced by injection of testosterone(75.00 mg/dog,i.m.)and estrogen(0.75 mg/dog,i.m.)on day 0(day of the first injection),day 21,day 42,and day 63.The doses of testosterone were doubled on days 21,42,and 63.The normal group did not receive any injection.Blood sampling was performed from the jugular vein at days 0,21,42,and 63.The concentrations of interleukin-8,interleukin-10,and tumor necrosis factor-α(TNF-α)were assayed by enzyme-linked immunosorbent assay.Results:The levels of interleukin-8,interleukin-10 and TNF-αwere not significantly different between the normal group and the benign prostate hyperplasia group.Also,concentrations of the pro-inflammatory cutokines were not significantly different between the normal group and the BPH group in each day of sampling.Conclusions:In spite of the induction of benign prostate hyperplasia,changes in the concentration of blood serum inflammatory cytokines were not significantly different with that of the normal group and between the days of induction of benign prostate hyperplasia during two months.It reveals that there is a stable state of serum inflammatory cytokines during induction of benign prostate hyperplasia in dogs.

Follicular fluid composition of ovulatory follicles in repeat breeder Holstein dairy cows

Objective: To examine differences in the metabolite, steroid and lipopolysaccharide of follicular fluid collected from the ovulatory follicle of Holstein repeat breeding cows, lactating cows, and virgin fertile heifers. Methods: Estrus was induced in animals possessing functional corpus luteum by intramuscular administration of prostaglandin F2α. Six to twelve hours after detection of the standing estrus, cervical samples were collected to detect subclinical endometritis via counting neutrophils on stained smears of cervical swabs. Then, follicular fluid of ovulatory follicles and serum samples were collected from repeat breeding cows (n=11), lactating cows (n=8) and virgin fertile heifers (n=10). Sodium and potassium were measured with a flame photometer method. Urea, total protein, glucose, cholesterol andβ-hydroxybutyric acid were assayed with commercial spectrophotometry kits. Chloride concentration was also measured with titration of samples against silver nitrate. Progesterone, estradiol-17βand lipopolysaccharide concentrations were measured using enzyme-linked immuno sorbent assay kits. Results: All analysis of follicular fluid samples showed that repeat breeding and lactating cows had a mean higher lipopolysaccharide concentration than that of the virgin fertile heifers (P<0.05). But concentration of serum estradiol-17βin repeat breeding and lactating cows was lower than that of virgin fertile heifers (P<0.05). In addition, the mean percentage of neutrophils in the cervical secretion of repeat breeding cows was higher than that of lactating cows (P<0.05). Conclusions: High follicular fluid concentration of lipopolysaccharide in ovulatory follicles results in the occurrence of repeat breeding syndrome in dairy cows. Further, a lower serum estradiol-17βconcentration and a higher percentage of neutrophil in the cervical secretion on the day of artificial insemination may have resulted in the occurrence of repeat breeding syndrome in dairy cows.

Treatment of cows with clinical endometritis Ⅲ as cows affected by pyometra-Non antibiotic treatment of severe clinical endometritis

Objective: To confirm the efficacy of non-antibiotic treatment with prostaglandin F2α(PGF2α) in dairy cows affected by severe clinical endometritis in (30±3) days in milk. Methods: Cows with clinical endometritis Ⅲ (n=399) were aligned into three groups randomly. The first group (n=115) received PGF2α, the second group (n=84) received intrauterine infusion (IUI) of oxytetracycline 10% + PGF2α, and the third group (n=200) received IUI. Cows were inseminated following estrus. The pregnancy status, parity, calving and artificial insemination season, ovaries with corpus luteum at the time of treatment, dystocia, body condition score and treatment groups were included in data analysis. Results: Total pregnancy rate was 40.1% after the first insemination and 94.2% after the third insemination. Overall pregnancy rate of treated cows with IUI+PGF2α(84.5%) was significantly lower than the treated cows with PGF2α(98.3%) or IUI (96%) (P<0.05). The first service pregnancy rate of inseminated cows in summer (14.3%) was lower in comparison with cows inseminated in spring (40.4%), fall (41.4%) and winter (51.7%) (P<0.05). Conclusions: PGF2α could treat severe clinical endometritis in dairy cows with corpus luteum in comparison with other treatments.

Effects of anestrus dog serum on superovulation in rats and mice

Objective:To study the effects of anestrus dog serum in rodents (contains high amount of FSH) compared with two common superovulatory programs.Methods: Rats (n=30) at diestrus phase were evenly divided into pregnant mare serum gonadotrophins (PMSG) group (administrated with 30 IU PMSG, and 48 h later following by 25 IU hCG), recombinant follicle stimulating hormone (rFSH) group (reducing dose every 12 h from 5 to 1 IU, then following by 25 IU hCG) and anestrus dog serum group (reducing dose every 12 h from 0.6 to 0.1 mL, then following by 25 IU of hCG). Mice (n=30) were also evenly divided into PMSG group (5 IU PMSG used at 13 pm, and 48 h later following by 5 IU of hCG), rFSH group (from 13 pm, reducing dose every 12 h from 2.5 to 0.5 IU, then following by 5 IU hCG) and anestrous dog serum group (from 13 pm, reducing dose every 12 h from 0.100 to 0.025 mL, then following by 5 IU hCG). Mice and rats placed with males for 24 h after last injection. Histology samples of ovaries were prepared and the numbers of corpus lutea were counted on day 14 after mating. Results: In mice, the differences among mean number of corpus lutea in all groups (P=0.01) and between FSH and dog serum group were significant (P=0.0007). But no significant differences were found between the mean number of corpus lutea in dog serum and PMSG, or between FSH and PMSG groups. In rats, mean number of corpus lutea were significant differences among three groups (P=0.01), and between PMSG and dog serum groups (P=0.02). Conclusions: Superovulatory response in anestrus dog serum group is similar to PMSG group in mice, which is relatively similar to FSH in rats.