Objective:In this study,an anti-haemorrhoid herbal recipe containing bark of Alstonia boonei and dried cloves–Syzygium aromaticum was investigated for cytogenotoxicity,mutagenicity and histopathological effects follo...Objective:In this study,an anti-haemorrhoid herbal recipe containing bark of Alstonia boonei and dried cloves–Syzygium aromaticum was investigated for cytogenotoxicity,mutagenicity and histopathological effects following the micronucleus and sperm morphology assays using Wistar albino rats.Methods:The rats were administered anti-haemorrhoid herbal recipe at 25.0%,50.0%and 100.0%,while distilled water and 0.05%sodium azide were the negative and positive controls,respectively.Phytochemicals in the herbal recipe were elucidated using the Fourier Transform Infrared(FTIR)and Gas Chromatography Flame Ionization Detection(GC-FID)techniques.Results:In the treated rats,there was increase in body weight,cells division inhibition,micronucleated polychromatic/normochromatic erythrocytes(MNPCEs&MNNCEs)and abnormal sperm cells were significantly induced at 100.0%(P<0.05),whereas 25.0%herbal recipe promoted cell division.The herbal recipe at 50.0%and 100.0%did not cause any histopathological damages on the liver,kidney and testis except at 25.0%.Polyphenolics,terpenoids and alkaloids were detected in this anti-haemorrhoid herbal recipe which could be cytotoxic,clastogenic and spermatotoxic at a high concentration(100.0%).Conclusion:These results necessitate regulation and control of consumption of the anti-haemorrhoid herbal recipe by people through the authorized government agencies in Nigeria.展开更多
文摘Objective:In this study,an anti-haemorrhoid herbal recipe containing bark of Alstonia boonei and dried cloves–Syzygium aromaticum was investigated for cytogenotoxicity,mutagenicity and histopathological effects following the micronucleus and sperm morphology assays using Wistar albino rats.Methods:The rats were administered anti-haemorrhoid herbal recipe at 25.0%,50.0%and 100.0%,while distilled water and 0.05%sodium azide were the negative and positive controls,respectively.Phytochemicals in the herbal recipe were elucidated using the Fourier Transform Infrared(FTIR)and Gas Chromatography Flame Ionization Detection(GC-FID)techniques.Results:In the treated rats,there was increase in body weight,cells division inhibition,micronucleated polychromatic/normochromatic erythrocytes(MNPCEs&MNNCEs)and abnormal sperm cells were significantly induced at 100.0%(P<0.05),whereas 25.0%herbal recipe promoted cell division.The herbal recipe at 50.0%and 100.0%did not cause any histopathological damages on the liver,kidney and testis except at 25.0%.Polyphenolics,terpenoids and alkaloids were detected in this anti-haemorrhoid herbal recipe which could be cytotoxic,clastogenic and spermatotoxic at a high concentration(100.0%).Conclusion:These results necessitate regulation and control of consumption of the anti-haemorrhoid herbal recipe by people through the authorized government agencies in Nigeria.
