OBJECTIVE:To aim at preliminary phytochemical screening of Daphne mucronata and evaluate its antioxidant and antibacterial activities.METHODS:Preliminary phytochemical screening was conducted for the crude extracts us...OBJECTIVE:To aim at preliminary phytochemical screening of Daphne mucronata and evaluate its antioxidant and antibacterial activities.METHODS:Preliminary phytochemical screening was conducted for the crude extracts using standard methods.Antioxidant properties of crude methanolic extracts,n-hexane,chloroform,ethyl acetate and aqueous fractions of leaves,roots and bark were evaluated following standard procedures.The antibacterial activities were checked against Acinetobacter baumannii(A.baumannii),Escherichia coli(E.coli),Pseudomonas aeruginosa(P.aeruginosa),Staphylococcus aureus(S.aureus),Morganella morganii(M.morganii)and vancomycin resistant S.aureus(VRSA).RESULTS:Carbohydrates,saponins,steroids,phenols/tannins,flavonoids and glycosides were present in different parts of Daphne mucronata.The extracts showed good antioxidant activity with EC50 values of 157.82-361.61μg/m L.Methanolic extracts of roots showed good activity against A.baumannii(86.95%),E.coli(85.18%)and S.aureus(84.61%).Methanolic extracts of bark were active against A.bumanni(65.21%),M.morganii(65.21%)and E.coli(62.96%).Methanolic extracts of leaves showed good activity against A.bumanni(78.26%),E.coli(77.78%),P.aeruginosa(74.07%),S.aureus(73.07%),M.morganii(69.56%),VRSA(68%)and Proteus vulgaris(60%).The n-hexane fraction of roots was effective against A.bumanni(78.26%).Chlorofom fraction of roots showed moderate activity against A.bumanni(60.86%)and S.aureus(61.53%).Ethyl acetate fraction of roots showed moderate activity against A.bumanii(69.56%),E.coli(62.96%)and S.aureus(69.23%).CONCLUSION:This study illustrates that Daphne mucronata possesses good antioxidant and antibacterial properties.The plant could be further exploited as potential natural antioxidant and as a new source of antimicrobials for treatment of various infections.展开更多
文摘OBJECTIVE:To aim at preliminary phytochemical screening of Daphne mucronata and evaluate its antioxidant and antibacterial activities.METHODS:Preliminary phytochemical screening was conducted for the crude extracts using standard methods.Antioxidant properties of crude methanolic extracts,n-hexane,chloroform,ethyl acetate and aqueous fractions of leaves,roots and bark were evaluated following standard procedures.The antibacterial activities were checked against Acinetobacter baumannii(A.baumannii),Escherichia coli(E.coli),Pseudomonas aeruginosa(P.aeruginosa),Staphylococcus aureus(S.aureus),Morganella morganii(M.morganii)and vancomycin resistant S.aureus(VRSA).RESULTS:Carbohydrates,saponins,steroids,phenols/tannins,flavonoids and glycosides were present in different parts of Daphne mucronata.The extracts showed good antioxidant activity with EC50 values of 157.82-361.61μg/m L.Methanolic extracts of roots showed good activity against A.baumannii(86.95%),E.coli(85.18%)and S.aureus(84.61%).Methanolic extracts of bark were active against A.bumanni(65.21%),M.morganii(65.21%)and E.coli(62.96%).Methanolic extracts of leaves showed good activity against A.bumanni(78.26%),E.coli(77.78%),P.aeruginosa(74.07%),S.aureus(73.07%),M.morganii(69.56%),VRSA(68%)and Proteus vulgaris(60%).The n-hexane fraction of roots was effective against A.bumanni(78.26%).Chlorofom fraction of roots showed moderate activity against A.bumanni(60.86%)and S.aureus(61.53%).Ethyl acetate fraction of roots showed moderate activity against A.bumanii(69.56%),E.coli(62.96%)and S.aureus(69.23%).CONCLUSION:This study illustrates that Daphne mucronata possesses good antioxidant and antibacterial properties.The plant could be further exploited as potential natural antioxidant and as a new source of antimicrobials for treatment of various infections.
