期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Genetic Variation among Fragmented Populations of Atriplex halimus L. Using Start Codon Targeted (SCoT) and ITS1-5.8S-ITS2 Region Markers
1
作者 asmaa elframawy Hisham Deif Ranya El-Bakatoushi 《American Journal of Molecular Biology》 2016年第2期101-115,共15页
Two forms of A. halimus shrubs: erect habit (A. halimus) and bushy habit shrub (A. schweinfurthii) are used naturally isolated by a considerable distance from each other and occupy the same area. To explore the effect... Two forms of A. halimus shrubs: erect habit (A. halimus) and bushy habit shrub (A. schweinfurthii) are used naturally isolated by a considerable distance from each other and occupy the same area. To explore the effect of natural isolation on the genetic basis of the two forms, Start Codon Targeted (SCoT) and the phylogenetic relationships of A. halimus by sequencing ITS1-5.8S-ITS2 regions of the ribosomal DNA are used. Significant isolation-by-distance relationship was found (r = 0.62, P = 0.001). Soil factors did not influence molecular variations. The natural isolation of A. halimus habitats restricts gene flow among the populations and the observed high within-population genetic diversity (74.19%) in this species is best explained by its outcrossing behaviour, long-lived individuals and overlapping generations. The UPGMA analysis of the SCoT results showed that all the studied populations were divided into two discrete genetic groups with significant separation of the two forms in Burg El-Arab area (Populations 1 and 2) and insignificant separation between two forms in El-Hammam area (population 5 and 6). The sequencing of the ITS1-5.8S-ITS2 rDNA regions also showed the insignificant separation of the two A. halimus forms. We conclude that gene flow depending on habitat fragmentation was the main factor affecting the population genetic differentiation. We suggest that the two forms do not merit specific rank in presence of interference between the two forms and absence of a breeding barrier fail to separate the different populations when they become sympatric. 展开更多
关键词 Atriplex halimus SCoT ITS1-5.8S-ITS2 rDNA Regions Atriplex schweinfurthii
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部