Objective: To evaluate the combination of several statins(atorvastatin, fluvastatin and simvastatin) and azoles(voriconazole, posaconazole and itraconazole) against Acanthamoeba spp. Methods: The efficiency of the dif...Objective: To evaluate the combination of several statins(atorvastatin, fluvastatin and simvastatin) and azoles(voriconazole, posaconazole and itraconazole) against Acanthamoeba spp. Methods: The efficiency of the different drug combinations against the trophozoite stage of different Acanthamoeba strains were evaluated by Alamar Blue assay. Effect on the cyst stage was observed by inverted microscope. Cytotoxicity of combinations of azoles and statins was evaluated by measuring the release of lactate dehydrogenase from a murine macrophage cell line. Results: Combinations of any of the tested statins and voriconazole or posaconazole were more efficient in inhibiting Acanthamoeba compared to statins or azoles individually. The drug combinations at the combined inhibitory concentrations 50% showed lower toxicity compared to that of the compounds alone.Conclusions: The combinations of statins together with voriconazole and posaconazole are more efficient than these drugs alone, and these combinations have lower cytotoxicity in mammalian cell lines.展开更多
基金supported by the grants Red de Investigation Colaborativa en Enfermedades Tropicales RICET(project no.RD16/0027/0001 of the programme of Redes Temáticas de Investigación Cooperativa,FIS),Spanish Ministry of Health,Madrid,SpainPI13/00490“Protozoosis Emergentes por Amebas de Vida Libre:Aislamiento,Caracterización,Nuevas Aproximaciones Terapéuticas y Traslación Clínica de los Resultados”from the Instituto de Salud Carlos III.IS+2 种基金supported by the Agustín de Betancourt Programmesupported by Ayudas Plan Propio de la Universidad de La Laguna 2018(proyectos puente I+D+i)supported by PI18/01380 from Instituto de Salud Carlos III and FEDER,Spain and CTQ2014-55888-C03-01/R(MINECO).MRB:RICET[RD16/0027/0001 project,from Programa Redes Temáticas de Investigación Cooperativa,FIS(Ministerio Español de Salud,Madrid,Spain).
文摘Objective: To evaluate the combination of several statins(atorvastatin, fluvastatin and simvastatin) and azoles(voriconazole, posaconazole and itraconazole) against Acanthamoeba spp. Methods: The efficiency of the different drug combinations against the trophozoite stage of different Acanthamoeba strains were evaluated by Alamar Blue assay. Effect on the cyst stage was observed by inverted microscope. Cytotoxicity of combinations of azoles and statins was evaluated by measuring the release of lactate dehydrogenase from a murine macrophage cell line. Results: Combinations of any of the tested statins and voriconazole or posaconazole were more efficient in inhibiting Acanthamoeba compared to statins or azoles individually. The drug combinations at the combined inhibitory concentrations 50% showed lower toxicity compared to that of the compounds alone.Conclusions: The combinations of statins together with voriconazole and posaconazole are more efficient than these drugs alone, and these combinations have lower cytotoxicity in mammalian cell lines.
