Vibrio harveyi cells(dose>103 cells mL-1)and extracellular products(ECP;>25μg mL-1 of total protein concentration)destroyed haematopoietic cultures of Nephrops norvegicus within 24 h of exposure.Cytopathic effe...Vibrio harveyi cells(dose>103 cells mL-1)and extracellular products(ECP;>25μg mL-1 of total protein concentration)destroyed haematopoietic cultures of Nephrops norvegicus within 24 h of exposure.Cytopathic effects(CPE)started after 4 h of exposure to the bacterial cells,with some granularity in the cytoplasm,mostly in cells in the outer periphe-ry of the explant growth.At the end of the infection,a considerable number of nuclei remained attached to the substrate,apparently unaffected.Following exposure to ECP,initial deterioration was observed at 2 h with the presence of granularity in the cytoplasm of<20%cells,and few cells displayed small vacuoles around the nuclei.Parallel results were obtained using whole animal experiments,with V.harveyi cells being lethal to nephrops within 24 h.展开更多
基金The support of European Union grant(FAIR-CI97-3413)is gratefully acknowledged.
文摘Vibrio harveyi cells(dose>103 cells mL-1)and extracellular products(ECP;>25μg mL-1 of total protein concentration)destroyed haematopoietic cultures of Nephrops norvegicus within 24 h of exposure.Cytopathic effects(CPE)started after 4 h of exposure to the bacterial cells,with some granularity in the cytoplasm,mostly in cells in the outer periphe-ry of the explant growth.At the end of the infection,a considerable number of nuclei remained attached to the substrate,apparently unaffected.Following exposure to ECP,initial deterioration was observed at 2 h with the presence of granularity in the cytoplasm of<20%cells,and few cells displayed small vacuoles around the nuclei.Parallel results were obtained using whole animal experiments,with V.harveyi cells being lethal to nephrops within 24 h.
