Measuring capillary oxygenation and the surrounding ultrastructure can allow one to monitor a microvascular niche and better understand crucial biological mechanisms.However,capillary oximetry and pericapillary ultras...Measuring capillary oxygenation and the surrounding ultrastructure can allow one to monitor a microvascular niche and better understand crucial biological mechanisms.However,capillary oximetry and pericapillary ultrastructure are challenging to measure in vivo.Here we demonstrate a novel optical imaging system,dual-band dual-scan inverse spectroscopic optical coherence tomography(D2-ISOCT),that,for the first time,can simultaneously obtain the following metrics in vivo using endogenous contrast:(1)capillary-level oxygen saturation and arteriolar-level blood flow rates,oxygen delivery rates,and oxygen metabolic rates;(2)spatial characteristics of tissue structures at length scales down to 30 nm;and(3)morphological images up to 2mm in depth.To illustrate the capabilities of D2-ISOCT,we monitored alterations to capillaries and the surrounding pericapillary tissue(tissue between the capillaries)in the healing response of a mouse ear wound model.The obtained microvascular and ultrastructural metrics corroborated well with each other,showing the promise of D2-ISOCT for becoming a powerful new non-invasive imaging tool.展开更多
Optical coherence tomography angiography relies on motion for contrast and requires at least two data acquisitions per pointwise scanning location.We present a method termed spectral contrast optical coherence tomogra...Optical coherence tomography angiography relies on motion for contrast and requires at least two data acquisitions per pointwise scanning location.We present a method termed spectral contrast optical coherence tomography angiography using visible light that relies on the spectral signatures of blood for angiography from a single scan using endogenous contrast.We demonstrate the molecular sensitivity of this method,which enables lymphatic vessel,blood,and tissue discrimination.展开更多
基金from the Evans Medical Foundation at Boston Medical Center,the National Institute of Health(R01CA173745,R01CA183101,and R01CA165309)the National Science Foundation(CBET-1240416).
文摘Measuring capillary oxygenation and the surrounding ultrastructure can allow one to monitor a microvascular niche and better understand crucial biological mechanisms.However,capillary oximetry and pericapillary ultrastructure are challenging to measure in vivo.Here we demonstrate a novel optical imaging system,dual-band dual-scan inverse spectroscopic optical coherence tomography(D2-ISOCT),that,for the first time,can simultaneously obtain the following metrics in vivo using endogenous contrast:(1)capillary-level oxygen saturation and arteriolar-level blood flow rates,oxygen delivery rates,and oxygen metabolic rates;(2)spatial characteristics of tissue structures at length scales down to 30 nm;and(3)morphological images up to 2mm in depth.To illustrate the capabilities of D2-ISOCT,we monitored alterations to capillaries and the surrounding pericapillary tissue(tissue between the capillaries)in the healing response of a mouse ear wound model.The obtained microvascular and ultrastructural metrics corroborated well with each other,showing the promise of D2-ISOCT for becoming a powerful new non-invasive imaging tool.
基金provided by the National Science Foundation Graduate Research Fellowship and the National Institute of Health(R01CA200064,R01CA183101,R01CA173745,R01CA165309)provided by the INCITE programthe Argonne Leadership Computing Facility,which is a DOE Office of Science User Facility supported under Contract DE-AC02-06CH11357.
文摘Optical coherence tomography angiography relies on motion for contrast and requires at least two data acquisitions per pointwise scanning location.We present a method termed spectral contrast optical coherence tomography angiography using visible light that relies on the spectral signatures of blood for angiography from a single scan using endogenous contrast.We demonstrate the molecular sensitivity of this method,which enables lymphatic vessel,blood,and tissue discrimination.
