Insecticidal Potential of α-Pinene and β-Caryophyllene against Myzus persicae and Their Impacts on Gene Expression

Myzus persicae(M.persicae)is now considered a threat to agricultural crops due to economic losses.Numerous synthetic insecticides applied every year against M.persicae,are reported to be unsafe for environment,humans,and beneficial insects.Furthermore,several species of Myzus have been found to develop resistance due to over application of these insecticides.Therefore,it is required to find some novel insecticide that would be safe for the environment as well as for humans.In the current study,two major pure constituentsα-pinene andβ-caryophyllene were evaluated for their insecticidal potential against M.persicae using a fumigant toxicity assay.Furthermore,impact ofα-pinene andβ-caryophyllene on expression of five different genes,e.g.,HSP 60,FPPS I,OSD,TOL and ANT responsible for reproduction,dispersion,and growth of M.persicae has also been investigated.To perform fumigant toxicity assay,five different concentrations(3.5,4,4.5,5 and 6μL L−1)ofα-pinene andβ-caryophyllene were prepared.Lethal concentration(LC)was calculated,and gene expression studies were executed through qRT PCR at LC30 ofα-pinene andβ-caryophyllene.Both constituents demonstrated excellent fumigant toxicity effects against M.persicae at all five concentrations.However,α-pinene shows significantly better results(98%)as compared toβ-caryophyllene(80%)after 72 h at 6μL L−1 of dose.The highest upregulation in expression was demonstrated at LC30 dose ofα-pinene in five in three out of five genes understudy(TOL,ANT,and FPPS I).Conversely,two genes HSP 60 and OSD demonstrated downregulation at LC30 dose ofβ-caryophyllene.Conclusively,our results highlighted the promising insecticidal potential of both compoundsα-pinene andβ-caryophylleneby interfering with the reproduction and development related processes in M.persicae,allowing us to recommend the phytoconstituents under investigation as an ecofriendly alternative to synthetic insecticides.

ExpressionHash: Securing Telecare Medical Information Systems Using BioHashing

The COVID-19 outbreak and its medical distancing phenomenon have effectively turned the global healthcare challenge into an opportunity for Telecare Medical Information Systems.Such systems employ the latest mobile and digital technologies and provide several advantages like minimal physical contact between patient and healthcare provider,easy mobility,easy access,consistent patient engagement,and cost-effectiveness.Any leakage or unauthorized access to users’medical data can have serious consequences for any medical information system.The majority of such systems thus rely on biometrics for authenticated access but biometric systems are also prone to a variety of attacks like spoong,replay,Masquerade,and stealing of stored templates.In this article,we propose a new cancelable biometric approach which has tentatively been named as“Expression Hash”for Telecare Medical Information Systems.The idea is to hash the expression templates with a set of pseudo-random keys which would provide a unique code(expression hash).This code can then be serving as a template for verication.Different expressions would result in different sets of expression hash codes,which could be used in different applications and for different roles of each individual.The templates are stored on the server-side and the processing is also performed on the server-side.The proposed technique is a multi-factor authentication system and provides advantages like enhanced privacy and security without the need for multiple biometric devices.In the case of compromise,the existing code can be revoked and can be directly replaced by a new set of expression hash code.The well-known JAFFE(The Japanese Female Facial Expression)dataset has been for empirical testing and the results advocate for the efcacy of the proposed approach.