目的构建含有小鼠血清和糖皮质激素诱导蛋白激酶3(serum and glucocorticoid-induced protein kinase 3,SGK3)基因的真核表达载体pcDNA3.1-MYC-SGK3-mCherry,并观察和验证其在转染细胞HEK293中的表达。方法通过聚合酶链式反应将实验室...目的构建含有小鼠血清和糖皮质激素诱导蛋白激酶3(serum and glucocorticoid-induced protein kinase 3,SGK3)基因的真核表达载体pcDNA3.1-MYC-SGK3-mCherry,并观察和验证其在转染细胞HEK293中的表达。方法通过聚合酶链式反应将实验室保存的真核表达质粒pcDNA3.1-MYC-SGK3中目的基因SGK3与mCherry融合并扩增出来,然后定向克隆至pcDNA3.1-MYC质粒中,经限制性内切酶消化和测序证实后,通过脂质体法转染HEK293细胞,Western blotting法检测目的基因的蛋白表达情况。结果测序结果与之前预期结果相符,证实pcDNA3.1-MYC-SGK3-mCherry真核表达载体构建成功。Western blotting结果显示,转染pcDNA3.1-MYC-SGK3-mCherry的HEK293细胞出现清晰的阳性反应条带,说明目的片段成功表达。结论pcDNA3.1-MYC-SGK3-mCherry真核表达载体构建成功。展开更多
The porous alumina have been prepared by anodization and an organic molecule 8-hydroxyquinoline aluminum (Alq3) has been impregnated in the pores. The morphology measured through atomic force microscopy shows that the...The porous alumina have been prepared by anodization and an organic molecule 8-hydroxyquinoline aluminum (Alq3) has been impregnated in the pores. The morphology measured through atomic force microscopy shows that the size of the pores is about 10 nm. A blue-shift photoluminescence of Alq3 in nanometer-sized hole is close to that of the monomers. The measured spectral characteristics demonstrate the limitation of the porous alumina to the emission of Alq3.展开更多
The ruthenium oxide nanoparticles with size less than 20 nm were fabricated by annealing the metallic ruthenium nanoparticles in air,which were synthesized by using the thermal reduction in the polyol solution.The rut...The ruthenium oxide nanoparticles with size less than 20 nm were fabricated by annealing the metallic ruthenium nanoparticles in air,which were synthesized by using the thermal reduction in the polyol solution.The rutile structure of the ruthenium oxide was proved by using transmission electron microscopy(TEM)and X-ray photoelectron spectroscopy(XPS).The oxide has good electron conductivity. The surface of the ruthenium oxide was modified by a vinyl silane coupling agent.The assembling of the silane to the oxide surface was proved by Infrared(IR)absorption spectroscopy.By mixing the nanoparticles with poly(methylvinylsiloxane)(PMVS)silicone rubber,a composite filled with dispersive conducting phase was fabricated.The temperature dependent conductivity shows that the electron transportation through composite is mainly dominated by tunneling.The measurement of piezoresistance shows that the composite at low strain has high piezoresistance repeatability.The 3D reconstruction images of the composite filled with carbon black or ruthenium oxide show that the aggregation of the nanoparticles differs much for two composites.The narrow distribution range of the particle size was thought to be the main factor for the high piezoresistance recurrence.展开更多
文摘目的构建含有小鼠血清和糖皮质激素诱导蛋白激酶3(serum and glucocorticoid-induced protein kinase 3,SGK3)基因的真核表达载体pcDNA3.1-MYC-SGK3-mCherry,并观察和验证其在转染细胞HEK293中的表达。方法通过聚合酶链式反应将实验室保存的真核表达质粒pcDNA3.1-MYC-SGK3中目的基因SGK3与mCherry融合并扩增出来,然后定向克隆至pcDNA3.1-MYC质粒中,经限制性内切酶消化和测序证实后,通过脂质体法转染HEK293细胞,Western blotting法检测目的基因的蛋白表达情况。结果测序结果与之前预期结果相符,证实pcDNA3.1-MYC-SGK3-mCherry真核表达载体构建成功。Western blotting结果显示,转染pcDNA3.1-MYC-SGK3-mCherry的HEK293细胞出现清晰的阳性反应条带,说明目的片段成功表达。结论pcDNA3.1-MYC-SGK3-mCherry真核表达载体构建成功。
基金This work was supported by the National Natural Science Foundation of China (Grant No. 60076002) and the Natural Science Foundation of Jiangsu Province (Grant No. BK99004).
文摘The porous alumina have been prepared by anodization and an organic molecule 8-hydroxyquinoline aluminum (Alq3) has been impregnated in the pores. The morphology measured through atomic force microscopy shows that the size of the pores is about 10 nm. A blue-shift photoluminescence of Alq3 in nanometer-sized hole is close to that of the monomers. The measured spectral characteristics demonstrate the limitation of the porous alumina to the emission of Alq3.
基金Supported by the National Natural Science Foundation of China(Grant No.10576008)
文摘The ruthenium oxide nanoparticles with size less than 20 nm were fabricated by annealing the metallic ruthenium nanoparticles in air,which were synthesized by using the thermal reduction in the polyol solution.The rutile structure of the ruthenium oxide was proved by using transmission electron microscopy(TEM)and X-ray photoelectron spectroscopy(XPS).The oxide has good electron conductivity. The surface of the ruthenium oxide was modified by a vinyl silane coupling agent.The assembling of the silane to the oxide surface was proved by Infrared(IR)absorption spectroscopy.By mixing the nanoparticles with poly(methylvinylsiloxane)(PMVS)silicone rubber,a composite filled with dispersive conducting phase was fabricated.The temperature dependent conductivity shows that the electron transportation through composite is mainly dominated by tunneling.The measurement of piezoresistance shows that the composite at low strain has high piezoresistance repeatability.The 3D reconstruction images of the composite filled with carbon black or ruthenium oxide show that the aggregation of the nanoparticles differs much for two composites.The narrow distribution range of the particle size was thought to be the main factor for the high piezoresistance recurrence.
