为解析卷叶木薯叶片异常发育的分子调控机制及代谢通路,以正常卷叶木薯叶片(JY)、突变株展叶叶片(ZY)和突变株卷叶叶片(BJ)为试验材料,基于转录组测序(RNA-seq)数据进行生物信息学分析。DESeq差异分析结果显示,ZY vs BJ、JY vs ZY、JY v...为解析卷叶木薯叶片异常发育的分子调控机制及代谢通路,以正常卷叶木薯叶片(JY)、突变株展叶叶片(ZY)和突变株卷叶叶片(BJ)为试验材料,基于转录组测序(RNA-seq)数据进行生物信息学分析。DESeq差异分析结果显示,ZY vs BJ、JY vs ZY、JY vs BJ分别有327(255个上调,72个下调)、1085(337个上调,748个下调)、689(381个上调,308个下调)个DEGs,有19个DEGs是3个比较组共同表达的。GO功能分析显示,DEGs在刺激反应、膜的组成部分、跨膜转运蛋白活性等途径差异显著。KEGG富集分析显示,DEGs在苯丙素的生物合成、植物激素信号转导、内质网中的蛋白加工等通路较为活跃。进一步对变异展叶与同株的卷叶和其他植株正常卷叶的DEGs分析发现集中富集到苯丙素的生物合成途径、淀粉和蔗糖代谢、植物激素信号转导,这可能是展叶突变体形成的关键因子。展叶与卷叶差异基因的主要KEGG代谢通路有9条,重要差异基因有9个。对不同类型叶片显微分析发现突变后的展叶木薯上表皮细胞层数增加、海绵组织结构变得疏松、维管束细胞减少。研究结果为进一步理解木薯叶片异常发育的分子机制提供了理论指导,为木薯遗传改良、种质创新挖掘提供基因资源和改良策略。展开更多
The electronic structures and optical properties of Y-doped ZnO are calculated using first-principles calculations.It is found that the replacement of Zn by the rare-earth element Y presents a shallow donor,and the Fe...The electronic structures and optical properties of Y-doped ZnO are calculated using first-principles calculations.It is found that the replacement of Zn by the rare-earth element Y presents a shallow donor,and the Fermi level moves into the conduction band(CB).The high dispersion and s-type character of CB is expected to result in an increase in conductivity.Moreover,the absorption spectrum of the Y-doped ZnO system exhibits a slight blue shift with an increase of Y concentration,and a higher transparency in visible light is expected.Therefore,the Y-doping in ZnO would enhance the mobility and hence increase the electrical conductivity without sacrificing the optical transparency,which is essential for the improvement of ZnO's behavior and its performance in extension applications.展开更多
Optical and electronic properties of Zn_(1−x)Mg_(x)O ternary alloys of wurtzite structure are calculated by using first-principles based on the framework of generalized gradient approximation to density functional the...Optical and electronic properties of Zn_(1−x)Mg_(x)O ternary alloys of wurtzite structure are calculated by using first-principles based on the framework of generalized gradient approximation to density functional theory with the introduction of the on-site Coulomb interaction.The use of the𝑈parameter on Zn-3d𝑑and O-2p𝑝orbits is obviously crucial,which can improve the GGA to predict the electronic properties and bandgap of the Zn_(1−x)Mg_(x)O(0≤𝑥≤0.25)system reasonably.It is further demonstrated that the bandgap widens with an increasing Mg concentration from 3.217 eV of ZnO to 3.877 eV of Zn0.75Mg0.25O.Therefore,the theoretical results show that Zn_(1−x)Mg_(x)O ternary alloys are potential candidates for optoelectronic materials,especially for UV photon emitters and detectors.展开更多
文摘为解析卷叶木薯叶片异常发育的分子调控机制及代谢通路,以正常卷叶木薯叶片(JY)、突变株展叶叶片(ZY)和突变株卷叶叶片(BJ)为试验材料,基于转录组测序(RNA-seq)数据进行生物信息学分析。DESeq差异分析结果显示,ZY vs BJ、JY vs ZY、JY vs BJ分别有327(255个上调,72个下调)、1085(337个上调,748个下调)、689(381个上调,308个下调)个DEGs,有19个DEGs是3个比较组共同表达的。GO功能分析显示,DEGs在刺激反应、膜的组成部分、跨膜转运蛋白活性等途径差异显著。KEGG富集分析显示,DEGs在苯丙素的生物合成、植物激素信号转导、内质网中的蛋白加工等通路较为活跃。进一步对变异展叶与同株的卷叶和其他植株正常卷叶的DEGs分析发现集中富集到苯丙素的生物合成途径、淀粉和蔗糖代谢、植物激素信号转导,这可能是展叶突变体形成的关键因子。展叶与卷叶差异基因的主要KEGG代谢通路有9条,重要差异基因有9个。对不同类型叶片显微分析发现突变后的展叶木薯上表皮细胞层数增加、海绵组织结构变得疏松、维管束细胞减少。研究结果为进一步理解木薯叶片异常发育的分子机制提供了理论指导,为木薯遗传改良、种质创新挖掘提供基因资源和改良策略。
基金Supported by the National Basic Research Program of China(No 2010CB631001)the National Natural Science Foundation of China(No 50871046)+1 种基金the Programs of Science and Technology Department of Heilongjiang Province(No QC2011C026)the Program for Changjiang Scholars and Innovative Research Team in University.
文摘The electronic structures and optical properties of Y-doped ZnO are calculated using first-principles calculations.It is found that the replacement of Zn by the rare-earth element Y presents a shallow donor,and the Fermi level moves into the conduction band(CB).The high dispersion and s-type character of CB is expected to result in an increase in conductivity.Moreover,the absorption spectrum of the Y-doped ZnO system exhibits a slight blue shift with an increase of Y concentration,and a higher transparency in visible light is expected.Therefore,the Y-doping in ZnO would enhance the mobility and hence increase the electrical conductivity without sacrificing the optical transparency,which is essential for the improvement of ZnO's behavior and its performance in extension applications.
基金by the National Natural Science Foundation of China under Grant No 50871046the National Basic Research Program of China(No 2010CB631001)the Program for Changjiang Scholars and Innovative Research Team in University.
文摘Optical and electronic properties of Zn_(1−x)Mg_(x)O ternary alloys of wurtzite structure are calculated by using first-principles based on the framework of generalized gradient approximation to density functional theory with the introduction of the on-site Coulomb interaction.The use of the𝑈parameter on Zn-3d𝑑and O-2p𝑝orbits is obviously crucial,which can improve the GGA to predict the electronic properties and bandgap of the Zn_(1−x)Mg_(x)O(0≤𝑥≤0.25)system reasonably.It is further demonstrated that the bandgap widens with an increasing Mg concentration from 3.217 eV of ZnO to 3.877 eV of Zn0.75Mg0.25O.Therefore,the theoretical results show that Zn_(1−x)Mg_(x)O ternary alloys are potential candidates for optoelectronic materials,especially for UV photon emitters and detectors.
