目的:研究肥大细胞表达膜蛋白1(mast cell expressed membrane protein 1,Mcemp1)对三阴性乳腺癌(triple-negative breast cancer,TNBC)中肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)极化状态、增殖及凋亡的影响。方法:生物...目的:研究肥大细胞表达膜蛋白1(mast cell expressed membrane protein 1,Mcemp1)对三阴性乳腺癌(triple-negative breast cancer,TNBC)中肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)极化状态、增殖及凋亡的影响。方法:生物学信息分析Mcemp1与肿瘤浸润免疫细胞相关性。构建鼠源性TAMs模型。Real-time RT-PCR验证TAMs模型,并检测沉默Mcemp1后对TAMs极化状态的影响。活细胞计数法检测沉默Mcemp1对TAMs增殖的影响。流式细胞术检测沉默Mcemp1对TA Ms细胞周期及凋亡的影响。结果:在乳腺癌中Mcemp1的表达与M0型巨噬细胞浸润呈正相关(P<0.05)。与单核巨噬细胞RW264.7相比,TAMs中CD206、IL-10的表达均显著升高(均P<0.05),IL-6、TNF的表达均显著下降(均P<0.05)。沉默Mcemp1的TAMs中IL-6、CXCL 9、CXCL10、IL-10、CD86和TNF的表达均显著上调(均P<0.05)。沉默Mcemp1的TAMs细胞数在第1、2天时无明显变化(均P>0.05),在第3、4天时增多(均P<0.05)。沉默Mcemp1使得TAMs的S期延长,G2/M期缩短(P<0.05)。沉默Mcemp1的TAMs凋亡率显著下降(P<0.05)。结论:Mcemp1在TNBC中TAMs具有免疫抑制的作用,下调其在TAMs的表达水平,可促进增殖并同时抑制细胞凋亡。展开更多
Based on the panel data of 30 provinces(autonomous regions,municipalities)in China from 2006 to 2019,the empirical study finds that the target values of economic growth expectation can promote high-quality economic de...Based on the panel data of 30 provinces(autonomous regions,municipalities)in China from 2006 to 2019,the empirical study finds that the target values of economic growth expectation can promote high-quality economic development,and there is an inverted U-shaped relationship between elastic target constraints and high-quality economic development.The elastic constraints can reduce the investment rate and improve the total factor productivity(TFP),which promotes high-quality economic development,but its mediating effect accounts for a small proportion.With the improvement of the marketization degree,the inhibiting effect of elastic constraints on high-quality economic development reduces.The analysis of the heterogeneity between the new or old normal and the levels of economic development shows that in the future,developed regions should set high target values and low elastic constraints,and underdeveloped regions should set high elastic constraints on the target values of economic growth expectation.展开更多
基金the Research on the Coordination between China's Interprovincial Population Aging and Economic Development from the Perspective of Spatio-Temporal Big Data,a project of the National Social Science Fund of China(No.20XRK003)the philosophy and social science collaborative innovation team from Chongqing colleges and universities named Chongqing Intelligent Finance Research Collaborative Innovation Team(No.2020016).
文摘Based on the panel data of 30 provinces(autonomous regions,municipalities)in China from 2006 to 2019,the empirical study finds that the target values of economic growth expectation can promote high-quality economic development,and there is an inverted U-shaped relationship between elastic target constraints and high-quality economic development.The elastic constraints can reduce the investment rate and improve the total factor productivity(TFP),which promotes high-quality economic development,but its mediating effect accounts for a small proportion.With the improvement of the marketization degree,the inhibiting effect of elastic constraints on high-quality economic development reduces.The analysis of the heterogeneity between the new or old normal and the levels of economic development shows that in the future,developed regions should set high target values and low elastic constraints,and underdeveloped regions should set high elastic constraints on the target values of economic growth expectation.