目的:探讨甲型流感病毒(IAV)对白细胞介素-35(IL-35)表达的影响。方法:采用酶联免疫吸附试验(ELISA)检测78例IAV感染患者(患者组)和80例健康体检者(健康对照组)血清IL-35的水平,以流感病毒H3N2感染人外周血单个核细胞(PBMC)设为流感细胞...目的:探讨甲型流感病毒(IAV)对白细胞介素-35(IL-35)表达的影响。方法:采用酶联免疫吸附试验(ELISA)检测78例IAV感染患者(患者组)和80例健康体检者(健康对照组)血清IL-35的水平,以流感病毒H3N2感染人外周血单个核细胞(PBMC)设为流感细胞组,未感染H3N2的PBMC为对照组,实时荧光定量PCR(RT-qPCR)和Western blot检测各组细胞中p35和EBI3mRNA和蛋白水平,ELISA检测细胞上清中IL-35的含量。结果:患者组IL-35血清水平较健康对照组高(78.40±28.90pg/ml vs 30.60±17.80pg/ml),流感细胞感染组EBI3和p35mRNA和蛋白表达较对照细胞组高(mRNA:EBI3为2.32±0.28vs 0.75±0.13,p35为1.83±0.23vs 0.94±0.17;蛋白:EBI3为1.25±0.11vs 0.56±0.06,p35为1.34±0.13vs 0.69±0.10),流感细胞感染组细胞培养液中IL-35亦高于对照细胞组(67.35±9.20pg/ml vs 25.62±6.40pg/ml),差异均有统计学意义(P<0.05)。结论:甲型流感病毒感染可引起体内IL-35表达升高。展开更多
To study the influence of construction interfaces on dynamic characteristics of roller compacted concrete dams(RCCDs),mechanical properties of construction interfaces are firstly analyzed. Then, the viscous-spring art...To study the influence of construction interfaces on dynamic characteristics of roller compacted concrete dams(RCCDs),mechanical properties of construction interfaces are firstly analyzed. Then, the viscous-spring artificial boundary(VSAB) is adopted to simulate the radiation damping of their infinite foundations, and based on the Marc software, a simplified seismic motion input method is presented by the equivalent nodal loads. Finally, based on the practical engineering of a RCC gravity dam, effects of radiation damping and construction interfaces on the dynamic characteristics of dams are investigated in detail. Analysis results show that dynamic response of the RCC gravity dam significantly reduces about 25% when the radiation damping of infinite foundation is considered. Hot interfaces and the normal cold interfaces have little influence on the dynamic response of the RCC gravity dam.However, nonlinear fracture along the cold interfaces at the dam heel will occur under the designed earthquake if the cold interfaces are combined poorly. Therefore, to avoid the fractures along the construction interfaces under the potential super earthquakes,combination quality of the RCC layers should be significantly ensured.展开更多
布里渊光时域分析(Brillouin Optical Time Domain Analysis,BOTDA)利用了布里渊受激放大特性,在测量精度和空间分辨率等方面有独特的优势。在应用基于BOTDA的分布式光纤传感技术对结构应变进行监测时,存在布里渊频移对应变和温度交叉...布里渊光时域分析(Brillouin Optical Time Domain Analysis,BOTDA)利用了布里渊受激放大特性,在测量精度和空间分辨率等方面有独特的优势。在应用基于BOTDA的分布式光纤传感技术对结构应变进行监测时,存在布里渊频移对应变和温度交叉敏感的问题。为了消除温度对应变监测的影响,基于BOTDA原理提出了一种温度补偿方法。结果表明该方法可通过附着成一体的应变和温度传感光纤的换算得到消除温度影响后的应变,无需得到待测物温度场,布设安装和数据处理均较方便,温度信息同步性和环境适应性较佳,且成本低廉,与现有温度补偿方法相比具有显著优势。通过对比室内水浴试验和某排水泵站现场试验的结果,可知基于BOTDA的光纤分布式传感温度补偿方法是有效的。展开更多
文摘目的:探讨甲型流感病毒(IAV)对白细胞介素-35(IL-35)表达的影响。方法:采用酶联免疫吸附试验(ELISA)检测78例IAV感染患者(患者组)和80例健康体检者(健康对照组)血清IL-35的水平,以流感病毒H3N2感染人外周血单个核细胞(PBMC)设为流感细胞组,未感染H3N2的PBMC为对照组,实时荧光定量PCR(RT-qPCR)和Western blot检测各组细胞中p35和EBI3mRNA和蛋白水平,ELISA检测细胞上清中IL-35的含量。结果:患者组IL-35血清水平较健康对照组高(78.40±28.90pg/ml vs 30.60±17.80pg/ml),流感细胞感染组EBI3和p35mRNA和蛋白表达较对照细胞组高(mRNA:EBI3为2.32±0.28vs 0.75±0.13,p35为1.83±0.23vs 0.94±0.17;蛋白:EBI3为1.25±0.11vs 0.56±0.06,p35为1.34±0.13vs 0.69±0.10),流感细胞感染组细胞培养液中IL-35亦高于对照细胞组(67.35±9.20pg/ml vs 25.62±6.40pg/ml),差异均有统计学意义(P<0.05)。结论:甲型流感病毒感染可引起体内IL-35表达升高。
基金Projects(20120094110005,20120094130003)supported by the Research Fund for the Doctoral Program of Higher Education of ChinaProjects(51379068,51139001,51279052,51209077,51179066)supported by the National Natural Science Foundation of China+1 种基金Project(NCET-11-0628)supported by the Program for New Century Excellent Talents in University,ChinaProjects(201201038,201101013)supported by the Public Welfare Industry Research Special Fund Project of Ministry of Water Resources of China
文摘To study the influence of construction interfaces on dynamic characteristics of roller compacted concrete dams(RCCDs),mechanical properties of construction interfaces are firstly analyzed. Then, the viscous-spring artificial boundary(VSAB) is adopted to simulate the radiation damping of their infinite foundations, and based on the Marc software, a simplified seismic motion input method is presented by the equivalent nodal loads. Finally, based on the practical engineering of a RCC gravity dam, effects of radiation damping and construction interfaces on the dynamic characteristics of dams are investigated in detail. Analysis results show that dynamic response of the RCC gravity dam significantly reduces about 25% when the radiation damping of infinite foundation is considered. Hot interfaces and the normal cold interfaces have little influence on the dynamic response of the RCC gravity dam.However, nonlinear fracture along the cold interfaces at the dam heel will occur under the designed earthquake if the cold interfaces are combined poorly. Therefore, to avoid the fractures along the construction interfaces under the potential super earthquakes,combination quality of the RCC layers should be significantly ensured.
文摘布里渊光时域分析(Brillouin Optical Time Domain Analysis,BOTDA)利用了布里渊受激放大特性,在测量精度和空间分辨率等方面有独特的优势。在应用基于BOTDA的分布式光纤传感技术对结构应变进行监测时,存在布里渊频移对应变和温度交叉敏感的问题。为了消除温度对应变监测的影响,基于BOTDA原理提出了一种温度补偿方法。结果表明该方法可通过附着成一体的应变和温度传感光纤的换算得到消除温度影响后的应变,无需得到待测物温度场,布设安装和数据处理均较方便,温度信息同步性和环境适应性较佳,且成本低廉,与现有温度补偿方法相比具有显著优势。通过对比室内水浴试验和某排水泵站现场试验的结果,可知基于BOTDA的光纤分布式传感温度补偿方法是有效的。