A sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu...A sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu-Pi decoction, and pure cinnamic acid using RP-HPLC. Di-Gu-Pi was extracted with 5% aqueous sodium bicarbonate, which was followed by purification with ion exchange column chromatography. The plasma samples taken from rats were deproteinized with methanol. The reversed-phase （HPLC） system with a Diamonsil C18 column and methanol-acetonitfile-water （8： 32： 60, volume ratio） （adjusted to pH = 3.0 with glacial acetic acid） as the mobile phase was employed for the separation of cinnamic acid in the plasma samples. The detection was set at 272 nm and 3-（p-fluoro-phenyl）-2-propenoic acid was chosen as the internal standard. The calibration curve was linear in a range from 0. 10 to 25.0μg/mL （R2 = 0. 9988, n = 9）. The precision was 3.42%-10. 10%; the between-day precision was 2. 84%-8.91% ; the accuracy was - 1.51%-1.26% ; the mean recovery was 99. 9%. The method was found to be sensitive, simple, accurate and appropriate for the determination of cinnamic acid.展开更多
1 Introduction As already known, complex prescription has been the outstanding feature of traditional Chinese medicine（TCM）. This fact therefore brings complicated formula with multicomponents from multimaterals, wh...1 Introduction As already known, complex prescription has been the outstanding feature of traditional Chinese medicine（TCM）. This fact therefore brings complicated formula with multicomponents from multimaterals, which produces some difficulties to detect the components and to control the quality. Shuanghuanglian preparations（SHLs）, including oral liquid, injection powder, capsule, and tablet, belong to a family of refined traditional Chinese patent medicine and a combined herbal remedy comprised of three herbs：展开更多
目的:通过建立草乌活性成分-作用靶点、蛋白相互作用、靶点相应的生物功能和通路网络,以及利用分子对接技术探讨草乌心脏毒性的作用机制。方法:通过中药系统药理学数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology,...目的:通过建立草乌活性成分-作用靶点、蛋白相互作用、靶点相应的生物功能和通路网络,以及利用分子对接技术探讨草乌心脏毒性的作用机制。方法:通过中药系统药理学数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology,TCMSP)和毒性与基因比较数据库(Comparative Toxicogenomics Database,CTD)筛选出草乌有毒候选成分。依据反向药效团匹配(Pharm Mapper)方法预测草乌毒性候选成分的作用靶点,与从人类基因数据库(Gene Cards)中寻找到的心脏相关基因蛋白进行比对,筛选出重合的蛋白作为草乌的潜在的心脏毒性靶点。采用Cytoscape软件构建草乌毒性候选成分-作用靶点网络。通过String数据库结合Cytoscape软件绘制蛋白相互作用网络,用DAVID平台对靶点生物功能及涉及的通路进行分析,最后用Discover Studio软件对关键蛋白与草乌毒性候选成分的结合进行验证。结果:草乌中筛选得到6种有毒候选成分,涉及27个心脏毒性作用靶点,网络分析结果表明靶点主要是通过参与心脏磷代谢,磷酸化的监管等磷相关的代谢和调节,以及通过FKBP1A,TGFB2,INSR等靶点对心脏的代谢,发育及形态产生重要的影响,进而产生心脏毒性。结论:利用中药多成分-多靶点-多通路的特点,探究了草乌心脏毒性作用机制,并预测了其可能存在的毒性,为进一步开展草乌心脏毒性作用机制研究提供了新思路和新方法。展开更多
文摘A sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu-Pi decoction, and pure cinnamic acid using RP-HPLC. Di-Gu-Pi was extracted with 5% aqueous sodium bicarbonate, which was followed by purification with ion exchange column chromatography. The plasma samples taken from rats were deproteinized with methanol. The reversed-phase （HPLC） system with a Diamonsil C18 column and methanol-acetonitfile-water （8： 32： 60, volume ratio） （adjusted to pH = 3.0 with glacial acetic acid） as the mobile phase was employed for the separation of cinnamic acid in the plasma samples. The detection was set at 272 nm and 3-（p-fluoro-phenyl）-2-propenoic acid was chosen as the internal standard. The calibration curve was linear in a range from 0. 10 to 25.0μg/mL （R2 = 0. 9988, n = 9）. The precision was 3.42%-10. 10%; the between-day precision was 2. 84%-8.91% ; the accuracy was - 1.51%-1.26% ; the mean recovery was 99. 9%. The method was found to be sensitive, simple, accurate and appropriate for the determination of cinnamic acid.
基金Supported by the National S&T Supporting Project in the 11th Five-Year Period(No.2006BAI08B04-01).
文摘1 Introduction As already known, complex prescription has been the outstanding feature of traditional Chinese medicine（TCM）. This fact therefore brings complicated formula with multicomponents from multimaterals, which produces some difficulties to detect the components and to control the quality. Shuanghuanglian preparations（SHLs）, including oral liquid, injection powder, capsule, and tablet, belong to a family of refined traditional Chinese patent medicine and a combined herbal remedy comprised of three herbs：
文摘目的:通过建立草乌活性成分-作用靶点、蛋白相互作用、靶点相应的生物功能和通路网络,以及利用分子对接技术探讨草乌心脏毒性的作用机制。方法:通过中药系统药理学数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology,TCMSP)和毒性与基因比较数据库(Comparative Toxicogenomics Database,CTD)筛选出草乌有毒候选成分。依据反向药效团匹配(Pharm Mapper)方法预测草乌毒性候选成分的作用靶点,与从人类基因数据库(Gene Cards)中寻找到的心脏相关基因蛋白进行比对,筛选出重合的蛋白作为草乌的潜在的心脏毒性靶点。采用Cytoscape软件构建草乌毒性候选成分-作用靶点网络。通过String数据库结合Cytoscape软件绘制蛋白相互作用网络,用DAVID平台对靶点生物功能及涉及的通路进行分析,最后用Discover Studio软件对关键蛋白与草乌毒性候选成分的结合进行验证。结果:草乌中筛选得到6种有毒候选成分,涉及27个心脏毒性作用靶点,网络分析结果表明靶点主要是通过参与心脏磷代谢,磷酸化的监管等磷相关的代谢和调节,以及通过FKBP1A,TGFB2,INSR等靶点对心脏的代谢,发育及形态产生重要的影响,进而产生心脏毒性。结论:利用中药多成分-多靶点-多通路的特点,探究了草乌心脏毒性作用机制,并预测了其可能存在的毒性,为进一步开展草乌心脏毒性作用机制研究提供了新思路和新方法。
