为更全面了解蚯蚓在环境生态领域应用的研究进展,本研究在Web of Science(WOS)核心合集数据库和中国知网(CNKI)数据库中检索2013—2023年以来蚯蚓在环境生态领域应用的有关文献,运用VOSviewer和CiteSpace工具进行统计和可视化分析。结...为更全面了解蚯蚓在环境生态领域应用的研究进展,本研究在Web of Science(WOS)核心合集数据库和中国知网(CNKI)数据库中检索2013—2023年以来蚯蚓在环境生态领域应用的有关文献,运用VOSviewer和CiteSpace工具进行统计和可视化分析。结果表明:1)中国发文总量居于世界第一,发展稳定。2)国内作者多为独立研究,国外作者团队交流合作密切。3)期刊Soil Biology&Biochemistry总被引次数和中心性最高,关于蚯蚓在环境生态领域应用的论文数量多且具有较高学术价值。4)中国科学院发文数量最多,在该领域研究表现突出。发文前10名的研究机构中7名来自法国,表明其在该领域的研究实力较雄厚。5)2013—2023年WOS和CNKI中有关蚯蚓在环境生态领域应用的文献研究方向重点和热点基本一致。蚯蚓种类以“赤子爱胜蚓(Eisenia fetida)”为主,研究前沿领域关键词集中于“氧化应激”“暴露”“降解”和“反应”。根据分析指出国内相关研究的优势与不足并提出对策与建议,预测了相关领域的发展前沿,可为今后蚯蚓在环境生态领域的研究和应用提供有价值的参考。展开更多
A plethora of information is available on the effects of salinity on plant growth and soil physico-chemical properties,but the effects on soil organisms are often neglected.Thus,a systematic investigation of how soil ...A plethora of information is available on the effects of salinity on plant growth and soil physico-chemical properties,but the effects on soil organisms are often neglected.Thus,a systematic investigation of how soil biodiversity,including bacteria,nematodes,mites,and earthworms,changes along saline gradients was conducted along the Bohai Sea coast at Laizhou City,Shandong Province,China,with 30 soil samples randomly selected and classified by salinity into two categories:saline and non-saline.Testing revealed a significantly higher abundance of the surveyed organisms in non-saline soils.The redundancy analysis showed that a negative correlation was observed between electrical conductivity and soil organism abundance in saline soil,but not in non-saline soil.Soil organic matter,available nitrogen,and total nitrogen all positively affected organism abundance in both saline and non-saline soils.The richness and Shannon diversity of nematodes were significantly higher in non-saline soils,but were not significantly different between soil types for other organisms.None of the environmental factors surveyed was obviously related to soil organism diversity.Consequently,our results suggested that soil electrical conductivity only negatively affected soil organisms in saline soil,while soil fertility positively affected soil organisms in both saline and non-saline soils.展开更多
Objective:To investigate the molecular mechanism underlying the anti-hepatic fibrosis activity of ethyl acetate fraction Dicliptera chinensis(L.)Juss.(EDC)in human hepatic stellate cells(HSCs)in vitro and in a carbon ...Objective:To investigate the molecular mechanism underlying the anti-hepatic fibrosis activity of ethyl acetate fraction Dicliptera chinensis(L.)Juss.(EDC)in human hepatic stellate cells(HSCs)in vitro and in a carbon tetrachloride(CCl4)-induced hepatic fibrosis mouse model in vivo.Methods:For in vitro study,HSCs were pre-treated with platelet-derived growth factor(10 ng/mL)for 2 h to ensure activation and treated with EDC for 24 h and 48 h,respectively.The effect of EDC on HSCs was assessed using cell counting kit-8 assay,EdU staining,transmission electron microscopy,immunofluorescence staining,and Western blot,respectively.For in vivo experiments,mice were intraperitoneally injected with CCl4(2μL/g,adjusted to a 25%concentration in olive oil),3 times per week for 6 weeks,to develop a hepatic fibrosis model.Forty 8-week-old male C57 BL/6 mice were divided into 4 groups using a random number table(n=10),including control,model,positive control and EDC treatment groups.Mice in the EDC and colchicine groups were intragastrically administered EDC(0.5 g/kg)or colchicine(0.2 mg/kg)once per day for 6 weeks.Mice in the control and model groups received an equal volume of saline.Biochemical assays and histological examinations were used to assess liver damage.Protein expression levels ofα-smooth muscle actin(α-SMA)and microtubule-associated protein light chain 3 B(LC3 B)were measured by Western blot.Results:EDC reduced pathological damage associated with liver fibrosis,downregulated the expression ofα-SMA and upregulated the expression of LC3 B(P<0.05),both in HSCs and the CCl4-induced liver fibrosis mouse model.The intervention of bafilomycin A1 and rapamycin in HSCs strongly supported the notion that inhibition of autophagy enhancedα-SMA protein expression levels(P<0.01).The results also found that the levels of phosphoinositide(PI3 K),p-PI3 K,AKT,p-AKT,mammalian target of rapamycin(mTOR),p-mTOR,and p-p70 S6 K all decreased after EDC treatment(P<0.05).Conclusion:EDC has anti-hepatic fibrosis activity by inducing autophagy and might be a potential drug to be further developed for human liver fibrosis therapy.展开更多
文摘为更全面了解蚯蚓在环境生态领域应用的研究进展,本研究在Web of Science(WOS)核心合集数据库和中国知网(CNKI)数据库中检索2013—2023年以来蚯蚓在环境生态领域应用的有关文献,运用VOSviewer和CiteSpace工具进行统计和可视化分析。结果表明:1)中国发文总量居于世界第一,发展稳定。2)国内作者多为独立研究,国外作者团队交流合作密切。3)期刊Soil Biology&Biochemistry总被引次数和中心性最高,关于蚯蚓在环境生态领域应用的论文数量多且具有较高学术价值。4)中国科学院发文数量最多,在该领域研究表现突出。发文前10名的研究机构中7名来自法国,表明其在该领域的研究实力较雄厚。5)2013—2023年WOS和CNKI中有关蚯蚓在环境生态领域应用的文献研究方向重点和热点基本一致。蚯蚓种类以“赤子爱胜蚓(Eisenia fetida)”为主,研究前沿领域关键词集中于“氧化应激”“暴露”“降解”和“反应”。根据分析指出国内相关研究的优势与不足并提出对策与建议,预测了相关领域的发展前沿,可为今后蚯蚓在环境生态领域的研究和应用提供有价值的参考。
基金supported by the National Science & Technology Pillar Program during the Twelfth Five-Year Plan Period of China(No.2012BAD14B01)the Ph.D.Programs Foundation of Ministry of Education of China(No.20110008130003)
文摘A plethora of information is available on the effects of salinity on plant growth and soil physico-chemical properties,but the effects on soil organisms are often neglected.Thus,a systematic investigation of how soil biodiversity,including bacteria,nematodes,mites,and earthworms,changes along saline gradients was conducted along the Bohai Sea coast at Laizhou City,Shandong Province,China,with 30 soil samples randomly selected and classified by salinity into two categories:saline and non-saline.Testing revealed a significantly higher abundance of the surveyed organisms in non-saline soils.The redundancy analysis showed that a negative correlation was observed between electrical conductivity and soil organism abundance in saline soil,but not in non-saline soil.Soil organic matter,available nitrogen,and total nitrogen all positively affected organism abundance in both saline and non-saline soils.The richness and Shannon diversity of nematodes were significantly higher in non-saline soils,but were not significantly different between soil types for other organisms.None of the environmental factors surveyed was obviously related to soil organism diversity.Consequently,our results suggested that soil electrical conductivity only negatively affected soil organisms in saline soil,while soil fertility positively affected soil organisms in both saline and non-saline soils.
基金Supported by the National Natural Science Foundation of China(No.81673774)the Administration of Traditional Chinese Medicine of Shandong Province(2019-0447)。
文摘Objective:To investigate the molecular mechanism underlying the anti-hepatic fibrosis activity of ethyl acetate fraction Dicliptera chinensis(L.)Juss.(EDC)in human hepatic stellate cells(HSCs)in vitro and in a carbon tetrachloride(CCl4)-induced hepatic fibrosis mouse model in vivo.Methods:For in vitro study,HSCs were pre-treated with platelet-derived growth factor(10 ng/mL)for 2 h to ensure activation and treated with EDC for 24 h and 48 h,respectively.The effect of EDC on HSCs was assessed using cell counting kit-8 assay,EdU staining,transmission electron microscopy,immunofluorescence staining,and Western blot,respectively.For in vivo experiments,mice were intraperitoneally injected with CCl4(2μL/g,adjusted to a 25%concentration in olive oil),3 times per week for 6 weeks,to develop a hepatic fibrosis model.Forty 8-week-old male C57 BL/6 mice were divided into 4 groups using a random number table(n=10),including control,model,positive control and EDC treatment groups.Mice in the EDC and colchicine groups were intragastrically administered EDC(0.5 g/kg)or colchicine(0.2 mg/kg)once per day for 6 weeks.Mice in the control and model groups received an equal volume of saline.Biochemical assays and histological examinations were used to assess liver damage.Protein expression levels ofα-smooth muscle actin(α-SMA)and microtubule-associated protein light chain 3 B(LC3 B)were measured by Western blot.Results:EDC reduced pathological damage associated with liver fibrosis,downregulated the expression ofα-SMA and upregulated the expression of LC3 B(P<0.05),both in HSCs and the CCl4-induced liver fibrosis mouse model.The intervention of bafilomycin A1 and rapamycin in HSCs strongly supported the notion that inhibition of autophagy enhancedα-SMA protein expression levels(P<0.01).The results also found that the levels of phosphoinositide(PI3 K),p-PI3 K,AKT,p-AKT,mammalian target of rapamycin(mTOR),p-mTOR,and p-p70 S6 K all decreased after EDC treatment(P<0.05).Conclusion:EDC has anti-hepatic fibrosis activity by inducing autophagy and might be a potential drug to be further developed for human liver fibrosis therapy.
