Catch organism assemblages along artificial reefs area and adjacent waters in Haizhou Bay

To better understand the community patterns mediated by connectivity in artificial reefs of coastal areas, it is necessary to understand the distribution and coexistence of organisms with artificial reefs area and adjacent waters. This study was conducted to examine main catches assemblages collected by trawls in Haizhou Bay,which included five habitats: the artificial reef area(AR), aquaculture area(AA), natural area(NA), estuary area(EA) and comprehensive effect area(CEA). The result shows that the total abundances of species in the five habitats were highly different(univariate PERMANOVA: P = 0.001, n = 24), but some species were also unique in their habitat(e.g. Scapharca subcrenata and Glossaulax didyma in AA). The body size distribution of specific species between habitats are different. For Collichthys lucidus, their body size in AR(14.63 cm ± 1.64 cm) and EA(14.3 cm ± 0.85 cm) is higher than that in NA(10.65 cm ± 1.64 cm), CEA(11.28 cm ± 1.85 cm) and AA(12.1 cm ±0.43 cm), which indicates the potential connection from AR to EA mediated by their adult population. We concluded that artificial reefs in AR can be considered key components that have the ability to support species assemblages in adjacent habitats. This study has implications for the conservation and monitoring of species assemblages in coastal areas in terms of that artificial reefs can be applied in different stages of habitat protection implementation and in different combinations of scenarios.

Effects of bone marrow mesenchymal stem cell combined with platelet-rich plasma treatment of bone nonunion after long bone fracture surgery on bone metabolism and cytokines

Objective: To study the effects of bone marrow mesenchymal stem cell combined with platelet-rich plasma treatment of bone nonunion after long bone fracture surgery on bone metabolism and cytokines. Methods: Patients who were treated in our hospital due to bone nonunion after long bone fracture surgery between March 2011 and October 2017 were selected and randomly divided into two groups, combined group received bone marrow mesenchymal stem cell combined with platelet-rich plasma therapy, and control group received bone marrow mesenchymal stem cell therapy. The levels of bone metabolism markers and growth cytokines in serum as well as the expression of bone metabolism-related signal molecules in peripheral blood were determined before treatment and 1 month after treatment. Results: Compared with those of same group before treatment, serum PINP, OPG, BALP, VEGF, TGF-β1, IGF-I, IGF-II and bFGF levels as well as peripheral blood Runx2, Wnt1, Wnt3a and β-catenin expression intensity of both groups of patients significantly increased whereas serum β-CTX and RANKL levels as well as peripheral blood NOX4 and NF-κB expression intensity significantly decreased after treatment, and serum PINP, OPG, BALP, VEGF, TGF-β1, IGF-I, IGF-II and bFGF levels as well as peripheral blood Runx2, Wnt1, Wnt3a and β-catenin expression intensity of combined group after treatment were higher than those of control group whereas serum β-CTX and RANKL levels as well as peripheral blood NOX4 and NF-κB expression intensity were lower than those of control group. Conclusion: Bone marrow mesenchymal stem cell combined with platelet-rich plasma treatment of bone nonunion after long bone fracture surgery can be more effective than bone marrow mesenchymal stem cell monotherapy to improve the bone metabolism and increase the cytokines.

Al−Mg−Mn−Er合金双面搅拌摩擦焊接头局部显微组织和强化机制

通过研究Al−Mg−Mn−Er合金双面搅拌摩擦焊接头的局部显微组织和强化机制揭示其软化机制。结果显示:焊核区形成细小等轴晶组织(5.61μm),而热机械影响区(45.32μm)和热影响区(100.73μm)仍为纤维状组织。从基体到焊核区,小角度晶界分数从75.6%降低到15.6%。退火效应导致位错密度从母材的1.8×10^(14)m^(−2)减小到焊核区的4.5×10^(12)m^(−2)。焊核区、热机械影响区和热影响区Al_(3)(Er,Zr)析出相的平均尺寸和体积分数与基体的(13.7 nm,0.13%)均接近。焊核区和热机械影响区的屈服强度最低,约为201 MPa;基体的屈服强度最高,约为295 MPa。位错强化和亚结构强化的损失是从基体到焊核区屈服强度降低的主要原因。

Advances and key focus areas in gastric cancer immunotherapy:A comprehensive scientometric and clinical trial review(1999-2023)

BACKGROUND Gastric cancer(GC)is the sixth most common cancer and third leading cause of cancer-related deaths worldwide.Current treatments mainly rely on surgery-and chemotherapy-based systemic;however,the prognosis remains poor for advanced disease.Recent studies have suggested that immunotherapy has significant potential in cancer therapy;thus,GC immunotherapy may improve quality of life and survival for patients with this disease.AIM To provide a comprehensive overview of the knowledge structure and research hotspots of GC immunotherapy.METHODS We conducted a bibliometric analysis of publications on immunotherapy related to GC in the Web of Science Core Collection database.We analyzed 2013 publications from 1999 to February 1,2023,using the VOSviewer and CiteSpace software.We assessed publication and citation distributions using the WoS platform and explored research countries,institutions,journals,authors,references,and keywords(co-occurrence,timeline view,and burst analysis).In addition,we examined 228 trials on immunotherapy,137 on adoptive cell therapy,274 on immune checkpoint inhibitors(ICIs),and 23 on vaccines from ClinicalTrials.gov and the International Clinical Trials Registry Platform.The Impact Index Per Article for the top ten high-cited papers collected from Reference Citation Analysis(RCA)are presented.RESULTS Our bibliometric analysis revealed that the study of immunotherapy in GC has developed rapidly in recent years.China accounted for almost half the publications,followed by the United States.The number of publications in recent years has been growing continuously,and most institutions and authors with the most publications are from China.The main keywords or clusters identified were“tumor microenvironment”,“adoptive immunotherapy”,“dendritic therapy”,and“microsatellite instability”.CONCLUSION Our analysis of 2013 publications indicated that immunotherapy for GC has led to several new developments in recent years.Considerable progress has been made in vaccinations,immune checkpoint therapy,and adoptive cellular therapy.In particular,ICIs and chimeric antigen receptor T-cells are novel options for the treatment of GC.We suggest that the combination of ICIs,chemotherapy,targeted therapy,and other immunotherapies should be the primary research direction in the future.

High expression of PD-L1 mainly occurs in non-small cell lung cancer patients with squamous cell carcinoma or poor differentiation

Background:Lung cancer is one of the most lethal cancers worldwide,but studies have shown that the higher the expression of programmed cell death protein 1 ligand 1(PD-L1)in non-small cell lung cancer(NSCLC),the more likely it will benefit from anti-PD-L1 immunotherapy.The purpose of our study was to collect and analyze abundant clinical samples in order to provide evidence for clinicians and patients who might consider anti-PD-L1 immunotherapy while jointly formulating treatment plans.Methods:On the one hand,we obtained cases from The Cancer Genome Atlas(TCGA)database,including 498 lung squamous cell cancer(LUSC)patients and 515 lung adenocarcinoma(LUAD)patients.We studied the lung caner driver gene in LUSC and LUAD.On the other hand,PD-L1 expression was detected in lung cancer tissues of 1,008 NSCLC patients with immunohistochemistry staining(IHC),and we studied the correlation between PD-L1 protein expression and clinicopathological characteristics.Results:PD-L1 expression was higher in LUSC than in LUAD at the mRNA level.In univariate analysis,PD-L1 expression at the protein level was higher in patients who were males,were LUSC,were smokers,had a tumor diameter>3 cm,had poor differentiation,or had stages Ⅲ-Ⅳ disease.In multivariate analysis,PD-L1 expression was higher in patients who were LUSC or in poor differentiation.Conclusion:In term of protein level,PD-L1 expression was higher in NSCLC patients who were LUSC or in poor differentiation.We recommend that PD-L1 IHC detection can be routinely performed in such populations that are likely to benefit most from PD-L1 immunotherapy.

Review of Design of Process Parameters for Squeeze Casting

Squeeze casting(SC)is an advanced net manufacturing process with many advantages for which the quality and properties of the manufactured parts depend strongly on the process parameters.Unfortunately,a universal efficient method for the determination of optimal process parameters is still unavailable.In view of the shortcomings and development needs of the current research methods for the setting of SC process parameters,by consulting and analyzing the recent research literature on SC process parameters and using the CiteSpace literature analysis software,manual reading and statistical analysis,the current state and characteristics of the research methods used for the determination of SC process parameters are summarized.The literature data show that the number of pub-lications in the literature related to the design of SC process parameters generally trends upward albeit with signifi-cant fluctuations.Analysis of the research focus shows that both“mechanical properties”and“microstructure”are the two main subjects in the studies of SC process parameters.With regard to materials,aluminum alloys have been extensively studied.Five methods have been used to obtain SC process parameters:Physical experiments,numeri-cal simulation,modeling optimization,formula calculation,and the use of empirical values.Physical experiments are the main research methods.The main methods for designing SC process parameters are divided into three categories:Fully experimental methods,optimization methods that involve modeling based on experimental data,and theoreti-cal calculation methods that involve establishing an analytical formula.The research characteristics and shortcomings of each method were analyzed.Numerical simulations and model-based optimization have become the new required methods.Considering the development needs and data-driven trends of the SC process,suggestions for the develop-ment of SC process parameter research have been proposed.

A systematic printability study of direct ink writing towards high-resolution rapid manufacturing

Direct ink writing(DIW)holds enormous potential in fabricating multiscale and multi-functional architectures by virtue of its wide range of printable materials,simple operation,and ease of rapid prototyping.Although it is well known that ink rheology and processing parameters have a direct impact on the resolution and shape of the printed objects,the underlying mechanisms of these key factors on the printability and quality of DIW technique remain poorly understood.To tackle this issue,we systematically analyzed the printability and quality through extrusion mechanism modeling and experimental validating.Hybrid non-Newtonian fluid inks were first prepared,and their rheological properties were measured.Then,finite element analysis of the whole DIW process was conducted to reveal the flow dynamics of these inks.The obtained optimal process parameters(ink rheology,applied pressure,printing speed,etc)were also validated by experiments where high-resolution(<100μm)patterns were fabricated rapidly(>70 mm s^(-1)).Finally,as a process research demonstration,we printed a series of microstructures and circuit systems with hybrid inks and silver inks,showing the suitability of the printable process parameters.This study provides a strong quantitative illustration of the use of DIW for the high-speed preparation of high-resolution,high-precision samples.

SRAFE:Siamese Regression Aesthetic Fusion Evaluation for Chinese Calligraphic Copy

Evaluation of calligraphic copy is the core of Chinese calligraphy appreciation and in-heritance.However,previous aesthetic evaluation studies often focussed on photos and paintings,with few attempts on Chinese calligraphy.To solve this problem,a Siamese regression aesthetic fusion method is proposed,named SRAFE,for Chinese calligraphy based on the combination of calligraphy aesthetics and deep learning.First,a dataset termed Evaluated Chinese Calligraphy Copies(E3C)is constructed for aesthetic evalu-ation.Second,12 hand‐crafted aesthetic features based on the shape,structure,and stroke of calligraphy are designed.Then,the Siamese regression network(SRN)is designed to extract the deep aesthetic representation of calligraphy.Finally,the SRAFE method is built by fusing the deep aesthetic features with the hand‐crafted aesthetic features.Experimental results show that scores given by SRAFE are similar to the aesthetic evaluation label of E3C,proving the effectiveness of the authors’method.

中学生膳食营养摄入与自我体重意识研究

[目的]研究中学生自我体重意识对膳食营养摄入的影响。[方法]以24h膳食回顾方法收集学生2d的膳食资料,同时以问卷收集学生自我体重意识相关信息。[结果]男生大部分营养素摄入显著高于女生;感觉偏胖的学生其能量、总脂肪、碳水化合物、维生素B1、烟酸、铁、锌的摄入量明显低于感觉偏瘦和感觉适中的学生;当学生对自身体重的满意程度依次从不满意、一般、到满意时,碳水化合物、膳食纤维、铁摄入量依次显著增高;打算减轻体重的学生其能量、蛋白质、总脂肪、碳水化合物、维生素B1、铁、锌均显著低于其他各组。[结论]应加强对中学生营养状况、体重的监测,并引导学生正确看待体重问题,避免盲目的体重控制和饮食限制。

血小板相关参数在慢性阻塞性肺疾病患者中的变化及意义

目的探究血小板在COPD患者与健康人之间是否存在差异及血小板相关参数变化的临床意义。方法选择该院122例初次入院被确诊为COPD且排除血液、肿瘤、肺血栓栓塞症等相关疾病的COPD住院患者作为COPD组,以性别、年龄、民族相匹配的该院同期健康体检者122例作为对照组,收集两组的临床资料及首次血常规检查结果。结果COPD组血小板计数为(193.85±66.68)×10~9/L、血小板与中性粒细胞比值为(48.97±25.15),对照组分别为(210.45±37.38)×10~9/L、(60.96±16.79),两组比较差异有统计学意义(P<0.05);COPD组平均血小板体积、血小板与淋巴细胞比值、中性粒细胞计数、白细胞计数均高于对照组(P<0.05),而血小板体积分布宽度、淋巴细胞计数、红细胞计数均低于对照组(P<0.05);血小板比容、嗜酸性粒细胞计数两组间差异无统计学意义(P>0.05)。血小板计数与气流受限严重程度不存在相关性(r_s=-0.104,P=0.252);血小板与中性粒细胞比同气流受限严重程度无相关(r_s=-0.070,P=0.444);血小板与淋巴细胞比同气流受限严重程度不存在相关(r_s=0.132,P=0.147);嗜酸性粒细胞计数与气流受限严重程度呈负相关(r_s=-0.267,P=0.003)。结论COPD患者血小板计数、血小板与中性粒细胞比值均降低,血小板计数、血小板与中性粒细胞比值同CODP患者疾病严重程度无关。

A nationwide survey of psychological distress among Chinese people in the COVID-19 epidemic:implications and policy recommendations

The Coronavirus Disease 2019(COVID-19)epidemic emerged in Wuhan,China,spread nationwide and then onto half a dozen other countries between December 2019 and early 2020.The implementation of unprecedented strict quarantine measures in China has kept a large number of people in isolation and affected many aspects of people's lives.It has also triggered a wide variety of psychological problems,such as panic disorder,anxiety and depression.This study is the first nationwide largescale survey of psychological distress in the general population of China during the COVID-19 epidemic.

Effect of gastrin on protein kinase C and its subtype in human colon cancer cell line SW480

INTRODUCTIONGastrin is atrophic gastrointestinal hormone whichis secreted by G cell.Gastrin has long beenconsidered a growth stimulatory hormone formucosa of the gastrointestinal tract.The growthresponses of certain colorectal cancer cells,andxenografts,can be stimulated by endogenousgastrin.Protein kinase C (PKC) is a family ofisozymes that plays a crucial role in transducingsignals of many hormones,growth peptides,

A nationwide survey of psychological distress among Chinese people in the COVID-19 epidemic: implications and policy recommendations

The Coronavirus Disease 2019(COVID-19)epidemic emerged in Wuhan,China,spread nationwide and then onto half a dozen other countries between December 2019 and early 2020.The implementation of unprecedented strict quarantine measures in China has kept a large number of people in isolation and affected many aspects of people’s lives.It has also triggered a wide variety of psychological problems,such as panic disorder,anxiety and depression.This study is the first nationwide largescale survey of psychological distress in the general population of China during the COVID-19 epidemic.

Expression profile of microRNAs in gastrointestinal stromal tumors revealed by high throughput quantitative RT-PCR microarray

AIM: To investigate the microRNA(miRNA) expression profile in gastrointestinal stromal tumor(GIST) tissues that could serve as a novel diagnostic biomarker for GIST detection.METHODS: We performed a quantitative real-time quantitative reverse transcriptase polymerase chain reaction assay to analyze the expression of 1888 miRNAs in a sample set that included 54 GIST tissue samples.RESULTS: We found that dysregulation of several miRNAs may be related to the malignant potential of GISTs. Six of these miRNAs, hsa-let-7c, miR-218,miR-488#, miR-4683, miR-34c-5p and miR-4773, were selected as the final list of biomarkers to separate the malignant GISTs(M group) from the benign GISTs(B group). In addition, MiR-29b-2#, hsa-let-7c, miR-891 b, miR-218, miR-204, miR-204-3p, miR-628-5p,miR-744, miR-29c#, miR-625 and miR-196 a were used to distinguish between the borderline(BO group) and M groups. There were 11 common miRNAs selected to separate the benign and borderline(BB) group from the M group, including hsa-let-7c, miR-218, miR-628-5p,miR-204-3p, miR-204, miR-891 b, miR-488#, miR-145,miR-891 a, miR-34c-5p and miR-196 a.CONCLUSION: The identified miRNAs appear tobe novel biomarkers to distinguish malignant from benign GISTs, which may be helpful to understand the mechanisms of GIST oncogenesis and progression,and to further elucidate the characteristics of GIST subtypes.

Attitudes towards acceptance of an innovative home-based and remote sensing rehabilitation protocol among cardiovascular patients in Shantou, China

BackgroundCardiac 康复(CR ) 协议有对多样化作为基于医院或基于中心的 CR 的一种选择包括本垒的心脏的电视康复(HBCTR ) 。采用本垒的心脏的电视康复的使用，向如此的电子健康技术的接受估计心脏病患者态度是必要的，特别在如此的技术的知识的中国， deficient.MethodsInterviews 在 Shantou 大学医学院的首先隶属于的医院里被进行， Shantou，中国。在经皮的冠的 interventional (一种总线标准) 以后外科，病人们完成了 survey.ResultsAmong 150 个病人，仅仅， 13% 曾经听说了 HBCTR。在我们的 HBCTR 节目的介绍以后， 60% 病人愿意参予节目。从我们问询表数据的 multivariate 分析，变老(或：0.92， 95% CI：0.86-0.98；P = 0.007 ) ，普通家庭月刊收入(或：0.13， 95% CI：0.05-0.34；P &#x0003c；0.001 ) ，教育水平(或：0.24， 95% CI：0.10-0.59；P = 0.002 ) 并且 physical&#x000a0；锻练时间(或：0.19， 95% CI：0.06-0.56；P = 0.003 ) 是为 HBCTR 的接受的独立预言者。从为参予的原因，病人们选择了：提高的安全和独立(28.3%) ，自我监视器的能力物理条件日报(25.4%) ，并且有自动并且紧急情况警戒(23.1%) 。推理因为拒绝：太拖累的操作(34.3%) 和不必要的协议(19.4%).ConclusionsMost 病人关于 HBCTR 缺乏知识，但是在他们了解了节目以后，自愿参与。几个个人和生活方式因素影响了他们节目的接受。这些显示两个都，技术的改进和节目的更好的理解将提高活跃参予。

基于非结构网格的不可压N-S方程多矩有限体积法（英文）

提出了一种基于三角形及四面体非结构网格的有限体积法(FVM),用以鲁棒且精确地求解不可压粘性流动问题。与传统的FVM方法仅将体积分平均值(VIA)作为计算变量的做法不同,本文提出的方法将VIA及点值(PV)同时作为计算变量并在每个迭代步进行计算更新。VIA以通量形式进行计算以确保数值守恒,PV可以通过控制方程的不同形式进行求解更新,无需守恒,因此可以采用非常高效的方法进行求解。将PV作为增加的变量使得紧致网格模板得以实现更高阶精度的重构,而且由此获得的数值模型对于非结构网格变得更鲁棒。本文针对二维/三维的三角形/四面体非结构网格提出了数值格式,给出了几个基准测试算例,验证了本文提出的数值方法在采用非结构网格求解不可压粘性流动问题时的精确性和鲁棒性。

Wernicke encephalopathy in a patient after liver transplantation: A case report

Wernicke encephalopathy(WE) is an acute neurological disorder resulting from vitamin B1 deficiency, which is common in chronic alcoholism and is rare in acute liver failure. So far, there are 2 cases of WE reported after liver transplantation. Here, we report a case of a 45-year-old nonalcoholic male patient who developed psychiatric and neurological disturbance 15 d after receiving orthotopic liver transplantation because of hepatitis B-related cirrhosis and portal hypertension. Brain magnetic resonance imaging(MRI) showed symmetric high-signal intensities in the periaqueductal area. The patient was diagnosed with WE and given intravenous high-dose vitamin B1 immediately. His neurological disturbance resolved in 7 d after receiving the vitamin B1. Brain MRI after 5 mo showed nearly complete recovery. Most WE cases may be misdiagnosed in patients after liver transplantation, and we should pay more attention to its onset.

Strategic mental health planning and its practice in China：retrospect and prospect

As a large country with a population of over 1.3 billion people,China has been widely recognized for its roles in economic development and for its participation in global governance.However,China’s efforts in domestic social governance receive both praise and blame.On one hand,the management of a large country that has 18%of the world population,is worth 15.5%of the world economic gross,and is made up of 56