Besides being critical components of marine food web,microorganisms play vital roles in biogeochemical cycling of nutrients and elements in the ocean.Currently little is known about microbial population structure and ...Besides being critical components of marine food web,microorganisms play vital roles in biogeochemical cycling of nutrients and elements in the ocean.Currently little is known about microbial population structure and their distributions in the eastern Indian Ocean.In this study,we applied molecular approaches including polymerase chain reaction-denaturant gradient gel electrophoresis（PCR-DGGE） and High-Throughput next generation sequencing to investigate bacterial 16S rRNA genes from the equatorial regions and the adjacent Bay of Bengal in the eastern Indian Ocean.In general,Bacteroidetes,Proteobacteria（mainly Alpha,and Gamma）,Actinobacteria,Cyanobacteria and Planctomycetes dominated the microbial communities.Horizontally distinct spatial distribution of major microbial groups was observed from PCR-DGGE gel image analyses.However,further detailed characterization of community structures by pyrosequencing suggested a more pronounced stratified distribution pattern：Cyanobacteria and Actinobacteria were more predominant at surface water（25m）;Bacteroidetes dominated at 25m and 150m while Proteobacteria（mainly Alphaproteobacteria） occurred more frequently at 75m water depth.With increasing water depth,the bacterial communities from different locations tended to share high similarity,indicating a niche partitioning for minor groups of bacteria recovered with high throughput sequencing approaches.This study provided the first ＂snapshot＂ on biodiversity and spatial distribution of Bacteria in water columns in the eastern Indian Ocean,and the findings further emphasized the potential functional roles of these microbes in energy and resource cycling in the eastern Indian Ocean.展开更多
基金The Program for New Century Excellent Talents in University under contract No.NCET-12-1065the National Natural Science Foundation of China under contract Nos 41276124 and 41176136+1 种基金the Science Fund for University Creative Research Groups in Tianjin under contract No.TD12-5003the Program for Changjiang Scholars to J Sun
文摘Besides being critical components of marine food web,microorganisms play vital roles in biogeochemical cycling of nutrients and elements in the ocean.Currently little is known about microbial population structure and their distributions in the eastern Indian Ocean.In this study,we applied molecular approaches including polymerase chain reaction-denaturant gradient gel electrophoresis（PCR-DGGE） and High-Throughput next generation sequencing to investigate bacterial 16S rRNA genes from the equatorial regions and the adjacent Bay of Bengal in the eastern Indian Ocean.In general,Bacteroidetes,Proteobacteria（mainly Alpha,and Gamma）,Actinobacteria,Cyanobacteria and Planctomycetes dominated the microbial communities.Horizontally distinct spatial distribution of major microbial groups was observed from PCR-DGGE gel image analyses.However,further detailed characterization of community structures by pyrosequencing suggested a more pronounced stratified distribution pattern：Cyanobacteria and Actinobacteria were more predominant at surface water（25m）;Bacteroidetes dominated at 25m and 150m while Proteobacteria（mainly Alphaproteobacteria） occurred more frequently at 75m water depth.With increasing water depth,the bacterial communities from different locations tended to share high similarity,indicating a niche partitioning for minor groups of bacteria recovered with high throughput sequencing approaches.This study provided the first ＂snapshot＂ on biodiversity and spatial distribution of Bacteria in water columns in the eastern Indian Ocean,and the findings further emphasized the potential functional roles of these microbes in energy and resource cycling in the eastern Indian Ocean.
