Aim: To evaluate the effects of the flavonoids extracted from the Semen Cuscutae (FSC) on the reproductive and en-docrine functions in male rats. Methods: (1) FSC were obtained from the semen of Cuscuta sinensis Lam t...Aim: To evaluate the effects of the flavonoids extracted from the Semen Cuscutae (FSC) on the reproductive and en-docrine functions in male rats. Methods: (1) FSC were obtained from the semen of Cuscuta sinensis Lam throughsolvent extraction and polyamide columnar chromatography; (2) Effect of FSC on the reproductive organs was assessedin immature rats. Rats were administered FSC through gastric gavage at a dose of 300 mg/kg per day for 7 days and theweights of testis, epididymis, seminal vesicle and pituitary gland were then observed; (3) To observe the effect of FSCon the reproductive endocrine function: same dose level of FSC was given to male rats of different age groups for 7days; on day 8, the plasma testosterone (T), estradiol (E_2) and LH were determined by RIA, the specific binding ofLH was estimated and the testes were weighed. (4) Effect of FSC on LH secretion was assessed in vitro on culturedadenohypophysis. (5) Effect of FSC on T secretion was assessed in vitro on Leydig cell culture. Results: FSC in-creased the weights of testis, epididymis and pituitary gland, and stimulated T and LH secretion both in vitro and inimmature rats. Conclusion: FSC invigorates the reproductive system and reproductive endocrine function in male rats.展开更多
文摘Aim: To evaluate the effects of the flavonoids extracted from the Semen Cuscutae (FSC) on the reproductive and en-docrine functions in male rats. Methods: (1) FSC were obtained from the semen of Cuscuta sinensis Lam throughsolvent extraction and polyamide columnar chromatography; (2) Effect of FSC on the reproductive organs was assessedin immature rats. Rats were administered FSC through gastric gavage at a dose of 300 mg/kg per day for 7 days and theweights of testis, epididymis, seminal vesicle and pituitary gland were then observed; (3) To observe the effect of FSCon the reproductive endocrine function: same dose level of FSC was given to male rats of different age groups for 7days; on day 8, the plasma testosterone (T), estradiol (E_2) and LH were determined by RIA, the specific binding ofLH was estimated and the testes were weighed. (4) Effect of FSC on LH secretion was assessed in vitro on culturedadenohypophysis. (5) Effect of FSC on T secretion was assessed in vitro on Leydig cell culture. Results: FSC in-creased the weights of testis, epididymis and pituitary gland, and stimulated T and LH secretion both in vitro and inimmature rats. Conclusion: FSC invigorates the reproductive system and reproductive endocrine function in male rats.
