OBJECTIVE: To evaluate the therapeutic effects of nape acupuncture combined with rehabilitative swallowing training for dysphagia caused by pseudobulbar palsy, and to compare it with rehabilitative swallowing training...OBJECTIVE: To evaluate the therapeutic effects of nape acupuncture combined with rehabilitative swallowing training for dysphagia caused by pseudobulbar palsy, and to compare it with rehabilitative swallowing training alone, and to observe the improvement in quality of life after the therapy.METHODS: One hundred patients were randomly divided into two groups: the rehabilitative swallowing training group(control group, n = 50) and the nape acupuncture combined with rehabilitative swallowing training group(experimental group,n = 50). Each group had 8 weeks' therapy, 5 times a week. Patients in the control group received rehabilitative swallowing training, while those in the experimental group received nape acupuncture therapy based on swallowing rehabilitation. The out-comes were assessed by the repetitive saliva-swallowing test(RSST), water swallow test(WST), standardized swallowing assessment(SSA), and a swallow quality-of-life questionnaire(SWAL-QOL). Correlations of onset age, onset frequency and lesion location with the efficacy of the acupuncture treatment were also observed.RESULTS: The scores for RSST, WST, and SSA in both groups were lower than before the therapy(P <0.001), although the changes were more marked in the experimental group than in the control group(RSST and WST, P < 0.005; SSA, P < 0.001). Both groups recorded changes in SWAL-QOL index after the therapy(P < 0.001); and the experimental group scored higher than the control group(P < 0.001). The efficacy of acupuncture was not correlated with location(P > 0.05), but was related to onset age(P < 0.05) and onset frequency(P < 0.01).CONCLUSION: Nape acupuncture combined with rehabilitative swallowing training has an effect on dysphagia caused by pseudobulbar palsy and improves quality of life.展开更多
Objective To investigate whether grain-sized moxibustion at Xinshu(BL15)and Shenshu(BL23)can alleviate cognitive decline and other pathologic features in early-stage Alzheimer disease(AD)using transgenic mice with 5 f...Objective To investigate whether grain-sized moxibustion at Xinshu(BL15)and Shenshu(BL23)can alleviate cognitive decline and other pathologic features in early-stage Alzheimer disease(AD)using transgenic mice with 5 familial AD mutations(5XFAD).Methods The genotype of transgenic mice was detected by polymerase chain reaction.A total of 40 transgenic mice(1.5 months old)were randomly and equally allocated to an AD model group(5XFAD group)or a grain-sized moxibustion group(5XFAD+GM group),with 20 wild-type(WT)mice(C57BL/6J)serving as the normal control group(WT group).Mice in the 5XFAD+GM group were treated by grain-sized moxibustion at bilateral Xinshu(BL15)and Shenshu(BL23).Mice in the WT group and 5XFAD group received no treatment but were restrained to ensure exposure to a similar experimental condition.Cognitive function and memory were assessed with the Morris water maze and Y-maze tests.The amyloidβ40(Aβ40)and amyloidβ42(Aβ42)levels in the brain were evaluated by enzyme-linked immunosorbent assay;amyloid plaque deposition in brain tissue sections was detected by thioflavin-S staining;the expression of glial fibrillary acidic protein(GFAP),cluster of differentiation 11b(CD11b),brain-derived neurotrophic factor(BDNF),and choline acetyltransferase(ChAT)in the hippocampus and prefrontal cortex was analyzed by immunohistochemistry.Results In the Morris water maze test,compared with the 5XFAD group,mice in the 5XFAD+GM group had a shorter escape latency and more target area crossings and spent more time in the target quadrant(P<0.05).In the Y-maze test,compared with the 5XFAD group,the number of training times of the 5XFAD+GM group was significantly decreased(P<0.05),together with more correct responses(P<0.05).Compared with the 5XFAD group,the levels of Aβ40 and Aβ42 in the brain tissue of the 5XFAD+GM group were significantly lower(P<0.05);in the hippocampus and prefrontal cortex,the total number of amyloidβplaque deposition were significantly lower(P<0.05);the expression levels of GFAP and CD11b were significantly reduced(P<0.05);and the expression levels of ChAT and BDNF were significantly increased(P<0.05).Conclusion Grain-sized moxibustion at Xinshu(BL15)and Shenshu(BL23)greatly improves learning and memory functions,decreases the levels of Aβ40 and Aβ42,inhibits amyloidβplaque deposition,decreases the expression of GFAP and CD11b,and increases the expression of ChAT and BDNF in AD mice to inhibit the progression of AD.展开更多
基金Supported by Zhejiang province administration of Traditional Chinese Medicine funded Project:Effects of nape acupuncture on dysphagia in pseudobulbar palsy in different stages(Project No:2014ZA094)
文摘OBJECTIVE: To evaluate the therapeutic effects of nape acupuncture combined with rehabilitative swallowing training for dysphagia caused by pseudobulbar palsy, and to compare it with rehabilitative swallowing training alone, and to observe the improvement in quality of life after the therapy.METHODS: One hundred patients were randomly divided into two groups: the rehabilitative swallowing training group(control group, n = 50) and the nape acupuncture combined with rehabilitative swallowing training group(experimental group,n = 50). Each group had 8 weeks' therapy, 5 times a week. Patients in the control group received rehabilitative swallowing training, while those in the experimental group received nape acupuncture therapy based on swallowing rehabilitation. The out-comes were assessed by the repetitive saliva-swallowing test(RSST), water swallow test(WST), standardized swallowing assessment(SSA), and a swallow quality-of-life questionnaire(SWAL-QOL). Correlations of onset age, onset frequency and lesion location with the efficacy of the acupuncture treatment were also observed.RESULTS: The scores for RSST, WST, and SSA in both groups were lower than before the therapy(P <0.001), although the changes were more marked in the experimental group than in the control group(RSST and WST, P < 0.005; SSA, P < 0.001). Both groups recorded changes in SWAL-QOL index after the therapy(P < 0.001); and the experimental group scored higher than the control group(P < 0.001). The efficacy of acupuncture was not correlated with location(P > 0.05), but was related to onset age(P < 0.05) and onset frequency(P < 0.01).CONCLUSION: Nape acupuncture combined with rehabilitative swallowing training has an effect on dysphagia caused by pseudobulbar palsy and improves quality of life.
基金This work was supported by the Natural Science Foundation of Zhejiang Province(浙江省自然科学基金,No.LY13H270002).
文摘Objective To investigate whether grain-sized moxibustion at Xinshu(BL15)and Shenshu(BL23)can alleviate cognitive decline and other pathologic features in early-stage Alzheimer disease(AD)using transgenic mice with 5 familial AD mutations(5XFAD).Methods The genotype of transgenic mice was detected by polymerase chain reaction.A total of 40 transgenic mice(1.5 months old)were randomly and equally allocated to an AD model group(5XFAD group)or a grain-sized moxibustion group(5XFAD+GM group),with 20 wild-type(WT)mice(C57BL/6J)serving as the normal control group(WT group).Mice in the 5XFAD+GM group were treated by grain-sized moxibustion at bilateral Xinshu(BL15)and Shenshu(BL23).Mice in the WT group and 5XFAD group received no treatment but were restrained to ensure exposure to a similar experimental condition.Cognitive function and memory were assessed with the Morris water maze and Y-maze tests.The amyloidβ40(Aβ40)and amyloidβ42(Aβ42)levels in the brain were evaluated by enzyme-linked immunosorbent assay;amyloid plaque deposition in brain tissue sections was detected by thioflavin-S staining;the expression of glial fibrillary acidic protein(GFAP),cluster of differentiation 11b(CD11b),brain-derived neurotrophic factor(BDNF),and choline acetyltransferase(ChAT)in the hippocampus and prefrontal cortex was analyzed by immunohistochemistry.Results In the Morris water maze test,compared with the 5XFAD group,mice in the 5XFAD+GM group had a shorter escape latency and more target area crossings and spent more time in the target quadrant(P<0.05).In the Y-maze test,compared with the 5XFAD group,the number of training times of the 5XFAD+GM group was significantly decreased(P<0.05),together with more correct responses(P<0.05).Compared with the 5XFAD group,the levels of Aβ40 and Aβ42 in the brain tissue of the 5XFAD+GM group were significantly lower(P<0.05);in the hippocampus and prefrontal cortex,the total number of amyloidβplaque deposition were significantly lower(P<0.05);the expression levels of GFAP and CD11b were significantly reduced(P<0.05);and the expression levels of ChAT and BDNF were significantly increased(P<0.05).Conclusion Grain-sized moxibustion at Xinshu(BL15)and Shenshu(BL23)greatly improves learning and memory functions,decreases the levels of Aβ40 and Aβ42,inhibits amyloidβplaque deposition,decreases the expression of GFAP and CD11b,and increases the expression of ChAT and BDNF in AD mice to inhibit the progression of AD.
