[Objectives]This study was conducted to investigate rapid propagation systems of Gynostemma pentaphyllum.[Methods]Ten different media were tested to select the optimal media for inducing callus proliferation,bud diffe...[Objectives]This study was conducted to investigate rapid propagation systems of Gynostemma pentaphyllum.[Methods]Ten different media were tested to select the optimal media for inducing callus proliferation,bud differentiation and rooting by using tissue culture technology,with G.pentaphyllum stem segments and leaves as explants.The stem segments of G.pentaphyllum were used as explants to directly induce rooting and germination,and appropriate media were selected.[Results]The optimum callus induction medium for G.pentaphyllum stem segments was MS+6-BA 1.0 mg/L+NAA 0.4 mg/L;the optimum rooting medium for stem callus was MS+NAA 0.2 mg/L;and the optimum germination medium for stem segments was MS+6-BA 1.0 mg/L.The optimum callus induction medium for G.pentaphyllum leaves was MS+6-BA 0.5 mg/L+NAA 0.2 mg/L;and the optimum rooting medium for leaves was MS+NAA 0.4 mg/L.The optimum rooting medium for G.pentaphyllum stem segments was MS+IAA 1.0 mg/L,with which the rooting rate was 100%,the average root length was about 3 cm,and the average number of sprouts per explant was 1.48,so it is the optimal condition.[Conclusions]This study provides a new method for in-vitro cultivation of G.pentaphyllum and has far-reaching significance for improving human health.展开更多
基金Supported by The China Agriculture Research System of MOF and MARA(CARS-21)Guangxi Innovation-Driven Development Project(GuiKe AA18242040)+2 种基金Special Foundation for National Science and Technology Basic Resources of China(2018FY100700)Guangxi Traditional Chinese Medicine Key Discipline Construction Project(GZXK-Z-20-67)Bagui Scholor Program of Guangxi Zhuang Autonomous Region and Research Innovation Team Project(GuiYaoChuang 2019005).
文摘[Objectives]This study was conducted to investigate rapid propagation systems of Gynostemma pentaphyllum.[Methods]Ten different media were tested to select the optimal media for inducing callus proliferation,bud differentiation and rooting by using tissue culture technology,with G.pentaphyllum stem segments and leaves as explants.The stem segments of G.pentaphyllum were used as explants to directly induce rooting and germination,and appropriate media were selected.[Results]The optimum callus induction medium for G.pentaphyllum stem segments was MS+6-BA 1.0 mg/L+NAA 0.4 mg/L;the optimum rooting medium for stem callus was MS+NAA 0.2 mg/L;and the optimum germination medium for stem segments was MS+6-BA 1.0 mg/L.The optimum callus induction medium for G.pentaphyllum leaves was MS+6-BA 0.5 mg/L+NAA 0.2 mg/L;and the optimum rooting medium for leaves was MS+NAA 0.4 mg/L.The optimum rooting medium for G.pentaphyllum stem segments was MS+IAA 1.0 mg/L,with which the rooting rate was 100%,the average root length was about 3 cm,and the average number of sprouts per explant was 1.48,so it is the optimal condition.[Conclusions]This study provides a new method for in-vitro cultivation of G.pentaphyllum and has far-reaching significance for improving human health.
