This study describes a method that not only generates an automatic and standardized crush injury in the skull base, but also provides investigators with the option to choose from a range of varying pressure revels. We...This study describes a method that not only generates an automatic and standardized crush injury in the skull base, but also provides investigators with the option to choose from a range of varying pressure revels. We designed an automatic, non-serrated forceps that exerts a varying force of 0 to 100 g and lasts for a defined period of 0 to 60 seconds. This device was then used to generate a crush injury to the right oculomotor nerve of dogs with a force of 10 g for 15 seconds, resulting in a deficit in the pupil-light reflex and ptosis. Further testing of our model with Toluidine-blue staining demonstrated that, at 2 weeks post-surgery disordered oculomotor nerve fibers, axonal loss, and a thinner than normal myelin sheath were visible. Electrophysiological examination showed occasional spontaneous potentials. Together, these data verified that the model for oculomotor nerve injury was successful, and that the forceps we designed can be used to establish standard mechanical injury models of peripheral nerves.展开更多
Dear Editor,Gliomas represent the most common primary brain tumors in adults and few targeted therapies are available.Thus,it’s imperative to explore novel target genes in gliomas.Fat mass and obesity-associated(FTO)...Dear Editor,Gliomas represent the most common primary brain tumors in adults and few targeted therapies are available.Thus,it’s imperative to explore novel target genes in gliomas.Fat mass and obesity-associated(FTO)gene encodes an alphaketoglutarate-dependent dioxygenase,which demethylates N6-methyladenosine on single-strand RNA.1 FTO is highly expressed in the brain but its potential contribution to gliomas is unknown.FOXO3a belongs to the forkhead family of transcription factors and it mediates important cellular processes like apoptosis and proliferation by regulating its target gene expression.2 Interestingly,genetic variants of FOXO33 and FTO4 are associated with longevity in human,suggesting that FTO and FOXO3a might share convergent effects in biological processes.Thus,we investigated the involvement of potential FTO–FOXO3a interaction in gliomas.展开更多
基金supported by grants from the National Natural Science Foundation of China, No. 30571907the International Science and Technology Cooperation Foundation of the Shanghai Committee of Science and Technology, China,No. 10410711400the Shanghai Scientific and Technical Committee Project, No. 05QMH1409
文摘This study describes a method that not only generates an automatic and standardized crush injury in the skull base, but also provides investigators with the option to choose from a range of varying pressure revels. We designed an automatic, non-serrated forceps that exerts a varying force of 0 to 100 g and lasts for a defined period of 0 to 60 seconds. This device was then used to generate a crush injury to the right oculomotor nerve of dogs with a force of 10 g for 15 seconds, resulting in a deficit in the pupil-light reflex and ptosis. Further testing of our model with Toluidine-blue staining demonstrated that, at 2 weeks post-surgery disordered oculomotor nerve fibers, axonal loss, and a thinner than normal myelin sheath were visible. Electrophysiological examination showed occasional spontaneous potentials. Together, these data verified that the model for oculomotor nerve injury was successful, and that the forceps we designed can be used to establish standard mechanical injury models of peripheral nerves.
基金supported by NSFC81500161,NSFC 81471317,Shanghai Science and Technology Committee Fund(No.16411967100)Medical and Industrial Cross-Research Fund of Shanghai Jiaotong University(YG2019QNB31).
文摘Dear Editor,Gliomas represent the most common primary brain tumors in adults and few targeted therapies are available.Thus,it’s imperative to explore novel target genes in gliomas.Fat mass and obesity-associated(FTO)gene encodes an alphaketoglutarate-dependent dioxygenase,which demethylates N6-methyladenosine on single-strand RNA.1 FTO is highly expressed in the brain but its potential contribution to gliomas is unknown.FOXO3a belongs to the forkhead family of transcription factors and it mediates important cellular processes like apoptosis and proliferation by regulating its target gene expression.2 Interestingly,genetic variants of FOXO33 and FTO4 are associated with longevity in human,suggesting that FTO and FOXO3a might share convergent effects in biological processes.Thus,we investigated the involvement of potential FTO–FOXO3a interaction in gliomas.
