Three small bandgap non-fullerene(SBG NFAs) acceptors,BDTI,BDTI-2 F and BDTI-4 F,based on a carbon-oxygen bridged central core and thieno[3,4-b]thiophene linker,end-capped with varied electronwithdrawing terminal grou...Three small bandgap non-fullerene(SBG NFAs) acceptors,BDTI,BDTI-2 F and BDTI-4 F,based on a carbon-oxygen bridged central core and thieno[3,4-b]thiophene linker,end-capped with varied electronwithdrawing terminal groups,were designed and synthesized.The acceptors exhibit strong absorption from 600 nm to 1000 nm.The optimal device incorporating designed NFA and PTB7-Th polymer donor achieves a power conversion efficiency of 9.11% with near 0 eV HOMO offset.The work presents a case study of efficient non-fullerene solar cells with small HOMO offsets,which is achieved by blending PTB7-Th with fine-tuned SBG acceptor.展开更多
As the open reading frames of hepatitis B virus(HBV)genomes are overlapping,resistance mutations(MTs)in HBV polymerase may result in stop codon MTs in hepatitis B surface proteins,which are usually detected as a mixed...As the open reading frames of hepatitis B virus(HBV)genomes are overlapping,resistance mutations(MTs)in HBV polymerase may result in stop codon MTs in hepatitis B surface proteins,which are usually detected as a mixed population with wild-type(WT)HBV.The question was raised how the coexistence of nucleos(t)ide analogs(NAs)resistance MTs and WT sequences affects HBV replication.In the present study,HBV genomes with frequently detected reverse transcriptase(RT)/surface truncation MTs,rtA181T/sW172^*,rtV191I/sW182^*and rtM204I/sW196^*,were phenotypically characterized alone or together with their WT counterparts in different ratios by transient transfection in the absence or presence of Nas.In the absence of Nas,RT/surface truncation MTs impaired the expression and secretion of HBV surface proteins,and had a dose-dependent negative effect on WT HBV virion secretion.However,in the presence of Nas,coexistence of MTs with WT maintained viral replication,and the presence of WT was able to rescue the production of MT HBV virions.Our findings reveal that complementation of WT and MT HBV genomes is highly effective under drug treatment.展开更多
The establishment of the National Key Ecological Function Areas(NKEFAs)is an important measure for national ecological security,but the current ecological and environmental evaluation of NKEFAs lacks research on the a...The establishment of the National Key Ecological Function Areas(NKEFAs)is an important measure for national ecological security,but the current ecological and environmental evaluation of NKEFAs lacks research on the air quality in the NKEFAs.This study presented the current status of the air quality in the NKEFAs and its driving factors using the geographic detector q-statistic method.The air quality in the NKEFAs was overall better than individual cities and urban agglomeration in eastern coast provinces of China,accounting for 9.21%of the days with air quality at Level III or above.The primary air pollutant was PM_(10),followed by PM_(2.5),with lower concentrations of the remaining pollutants.Pollution was more severe in the sand fixation areas,where air pollution was worst in spring and best in autumn,contrasting with other NKEFAs and individual cities and urban agglomerations.The main influencing factors of air quality index(AQI)in the NKEFAs were land use type,wind speed,and relative humidity also weighted more heavily than factors such as industrial pollution and anthropogenic emissions,and most of these influence factors have two types of interactive effects:binary and nonlinear enhancements.These results indicated that air pollution in the NKEFAs was not related with the emission by intensive economic development.Thus,the policies taking the NKEFAs as restricted development zones were effective,but the air pollution caused by PM_(10) also showed the ecological status in the NKEFAs,especially at sand fixation areas was not quite optimistic,and more strict environmental protection measures should be taken to improve the ecological status in these NKEFAs.展开更多
Net area change analysis can dramatically underestimate total change of land cover, even sometimes seriously misinterpret ecological processes of the ecosystem, especially in arid or semiarid zones. In this paper, a s...Net area change analysis can dramatically underestimate total change of land cover, even sometimes seriously misinterpret ecological processes of the ecosystem, especially in arid or semiarid zones. In this paper, a suite of indices are presented to characterize land-cover swaps that may seriously damage the ecosystem in arid or semiarid zones, based on swap-change areas extracted from remotely sensed images. First, swap percentage of total area and swap intensity of total changes were used to determine the status of land-cover swap change in an area. Then, dominated swap category and individual swap- change intensity for a land-cover category were used to determine flagged land-cover swap-change categories. Finally, swap-change mode and Pielou's index were used to determine the land-cover swap-change processes of dominant categories. A case study is conducted using this approach, based on two land-cover maps in the 1980s and 2000 in Naiman Qi, Tongliao City, Inner Mongolia, China. This study shows that the approach can clearly quantify the severity and flagged classes of land-cover swap-change and reveal their relationship with ecological processes of the ecosystem. These results indicate that the approach can give deep insights into swap change, which can be very valuable to land-cover policy making and management.展开更多
Activatable prodrugs have received considerable attention in cancer therapy due to their high specificity and reduced side effects.However,the theranostic prodrug with multiple cancerous organelles targeting and combi...Activatable prodrugs have received considerable attention in cancer therapy due to their high specificity and reduced side effects.However,the theranostic prodrug with multiple cancerous organelles targeting and combinational therapy is still rare.In this report,an esterase-responsive prodrug tetraphenylethylene functionalized quinolinium-ester-chlorambucil(TPEQC)was developed for dual organelles-targeted and image-guided cancer therapy through synergetic chemotherapy(CT)and photodynamic therapy(PDT).TPE-QC was constructed by conjugating an anticancer drug of chlorambucil with an aggregation-induced emission active photosensitizer of tetraphenylethylene functionalized hydroxyethyl quinolinium(TPE-QO)via the hydrolyzable ester linkage.The fluorescence and photosensitization of TPE-QC were initially quenched because of the photoinduced electron transfer(PET)effect.After reacting with esterase,the ester group of TPE-QC could be selectively hydrolyzed to release chlorambucil and TPE-QO,which terminated the PET process and switched on the fluorescence and photosensitization.Benefitting from the overexpressed esterase in cancer cells,TPE-QC could be efficiently activated in cancer cells rather than in normal cells,while the restoredfluorescence could preciselymonitor the release of TPE-QC.Importantly,activated TPE-QC accumulated in both organelles of lysosome and mitochondria,resulting in enhanced anticancer potency.In vivo experiments demonstrated that TPE-QC displayed efficient tumor microenvironment-activatable features and excellent tumor therapeutic effects through combinational CT and PDT.展开更多
基金the National Key R&D Program of China (2017YFA0204701)Strategic Priority Research Program of the Chinese Academy of Sciences (XDB12010200)+1 种基金National Basic Research Program of China (Program 973) (No. 2014CB643502)the National Natural Science Foundation of China (21572234, 21661132006, 91833304, 21402194) for their financial support。
文摘Three small bandgap non-fullerene(SBG NFAs) acceptors,BDTI,BDTI-2 F and BDTI-4 F,based on a carbon-oxygen bridged central core and thieno[3,4-b]thiophene linker,end-capped with varied electronwithdrawing terminal groups,were designed and synthesized.The acceptors exhibit strong absorption from 600 nm to 1000 nm.The optimal device incorporating designed NFA and PTB7-Th polymer donor achieves a power conversion efficiency of 9.11% with near 0 eV HOMO offset.The work presents a case study of efficient non-fullerene solar cells with small HOMO offsets,which is achieved by blending PTB7-Th with fine-tuned SBG acceptor.
基金supported by the Deutsche Forschungsgemeinschaft (TRR60)the National Nature Science Foundation of China (31770180, 31621061)+1 种基金the Youth Innovation Promotion Association CAS (No. 2016303)the Youth Planning Project of Hubei Health Planning Commission (WJ2017Q027)
文摘As the open reading frames of hepatitis B virus(HBV)genomes are overlapping,resistance mutations(MTs)in HBV polymerase may result in stop codon MTs in hepatitis B surface proteins,which are usually detected as a mixed population with wild-type(WT)HBV.The question was raised how the coexistence of nucleos(t)ide analogs(NAs)resistance MTs and WT sequences affects HBV replication.In the present study,HBV genomes with frequently detected reverse transcriptase(RT)/surface truncation MTs,rtA181T/sW172^*,rtV191I/sW182^*and rtM204I/sW196^*,were phenotypically characterized alone or together with their WT counterparts in different ratios by transient transfection in the absence or presence of Nas.In the absence of Nas,RT/surface truncation MTs impaired the expression and secretion of HBV surface proteins,and had a dose-dependent negative effect on WT HBV virion secretion.However,in the presence of Nas,coexistence of MTs with WT maintained viral replication,and the presence of WT was able to rescue the production of MT HBV virions.Our findings reveal that complementation of WT and MT HBV genomes is highly effective under drug treatment.
基金This work was supported by the National Key Research and Development Plan of China(Grant No.2016YFC0500205)the Research on Multi_Level Complex Spatial Data Model and the Consistency(No.41571391).
文摘The establishment of the National Key Ecological Function Areas(NKEFAs)is an important measure for national ecological security,but the current ecological and environmental evaluation of NKEFAs lacks research on the air quality in the NKEFAs.This study presented the current status of the air quality in the NKEFAs and its driving factors using the geographic detector q-statistic method.The air quality in the NKEFAs was overall better than individual cities and urban agglomeration in eastern coast provinces of China,accounting for 9.21%of the days with air quality at Level III or above.The primary air pollutant was PM_(10),followed by PM_(2.5),with lower concentrations of the remaining pollutants.Pollution was more severe in the sand fixation areas,where air pollution was worst in spring and best in autumn,contrasting with other NKEFAs and individual cities and urban agglomerations.The main influencing factors of air quality index(AQI)in the NKEFAs were land use type,wind speed,and relative humidity also weighted more heavily than factors such as industrial pollution and anthropogenic emissions,and most of these influence factors have two types of interactive effects:binary and nonlinear enhancements.These results indicated that air pollution in the NKEFAs was not related with the emission by intensive economic development.Thus,the policies taking the NKEFAs as restricted development zones were effective,but the air pollution caused by PM_(10) also showed the ecological status in the NKEFAs,especially at sand fixation areas was not quite optimistic,and more strict environmental protection measures should be taken to improve the ecological status in these NKEFAs.
文摘Net area change analysis can dramatically underestimate total change of land cover, even sometimes seriously misinterpret ecological processes of the ecosystem, especially in arid or semiarid zones. In this paper, a suite of indices are presented to characterize land-cover swaps that may seriously damage the ecosystem in arid or semiarid zones, based on swap-change areas extracted from remotely sensed images. First, swap percentage of total area and swap intensity of total changes were used to determine the status of land-cover swap change in an area. Then, dominated swap category and individual swap- change intensity for a land-cover category were used to determine flagged land-cover swap-change categories. Finally, swap-change mode and Pielou's index were used to determine the land-cover swap-change processes of dominant categories. A case study is conducted using this approach, based on two land-cover maps in the 1980s and 2000 in Naiman Qi, Tongliao City, Inner Mongolia, China. This study shows that the approach can clearly quantify the severity and flagged classes of land-cover swap-change and reveal their relationship with ecological processes of the ecosystem. These results indicate that the approach can give deep insights into swap change, which can be very valuable to land-cover policy making and management.
基金supported by National Natural Science Foundation of China(nos.21975149,22077077 and 21672135)Funded Projects for the Academic Leaders and Academic Backbones,Shaanxi Normal University(no.18QNGG007)the Fundamental Research Funds for the Central Universities(nos.GK201902006 and GK202003036).
文摘Activatable prodrugs have received considerable attention in cancer therapy due to their high specificity and reduced side effects.However,the theranostic prodrug with multiple cancerous organelles targeting and combinational therapy is still rare.In this report,an esterase-responsive prodrug tetraphenylethylene functionalized quinolinium-ester-chlorambucil(TPEQC)was developed for dual organelles-targeted and image-guided cancer therapy through synergetic chemotherapy(CT)and photodynamic therapy(PDT).TPE-QC was constructed by conjugating an anticancer drug of chlorambucil with an aggregation-induced emission active photosensitizer of tetraphenylethylene functionalized hydroxyethyl quinolinium(TPE-QO)via the hydrolyzable ester linkage.The fluorescence and photosensitization of TPE-QC were initially quenched because of the photoinduced electron transfer(PET)effect.After reacting with esterase,the ester group of TPE-QC could be selectively hydrolyzed to release chlorambucil and TPE-QO,which terminated the PET process and switched on the fluorescence and photosensitization.Benefitting from the overexpressed esterase in cancer cells,TPE-QC could be efficiently activated in cancer cells rather than in normal cells,while the restoredfluorescence could preciselymonitor the release of TPE-QC.Importantly,activated TPE-QC accumulated in both organelles of lysosome and mitochondria,resulting in enhanced anticancer potency.In vivo experiments demonstrated that TPE-QC displayed efficient tumor microenvironment-activatable features and excellent tumor therapeutic effects through combinational CT and PDT.