Objective： The aim of the study was to establish models of Hep-2 laryngeal cancer cell line of different oxygen supplying, trying to investigate the impact of normoxia, hypoxia, reoxygenation after hypoxia on apoptos...Objective： The aim of the study was to establish models of Hep-2 laryngeal cancer cell line of different oxygen supplying, trying to investigate the impact of normoxia, hypoxia, reoxygenation after hypoxia on apoptosis and expression of proteins HIF-1α and p53 to Hep-2 human laryngeal cancer cell line induced by ^60Co γ-ray. Methods： Human laryngeal cancer Hep-2 cells were divided into 3 groups： group A （normoxia）, group B （hypoxia）, and group C （reoxygenation after hypoxia）. All of the cells were exposed to 5 Gy dosage of γ-ray. Flow cytometry （FCM） was used tomeasure the protein levels of HIF-1α and p53 and to detect cell apoptosis. The protein levels of HIF-la and p53 were also determined by immunohistochemistry and Western blotting. The expression of HIF-la mRNA was determined by RT-PCR. Results： The protein levels of HIF-1α and p53 were evidently increased in group B compared to group A. The protein levels of HIF-1α and p53 in group C were lower compared to group B; the rate of apoptosis in group C was higher than that in group B. Conclusion： Hypoxia decreased the effect of apoptosis induced by ^60Co γ-ray in Hep-2 human laryngeal cancer cell line. The apoptosis pathway maybe related to some other genes or proteins but not p53 in the conditions of hypoxia and reoxygenation after hypoxia.展开更多
文摘Objective： The aim of the study was to establish models of Hep-2 laryngeal cancer cell line of different oxygen supplying, trying to investigate the impact of normoxia, hypoxia, reoxygenation after hypoxia on apoptosis and expression of proteins HIF-1α and p53 to Hep-2 human laryngeal cancer cell line induced by ^60Co γ-ray. Methods： Human laryngeal cancer Hep-2 cells were divided into 3 groups： group A （normoxia）, group B （hypoxia）, and group C （reoxygenation after hypoxia）. All of the cells were exposed to 5 Gy dosage of γ-ray. Flow cytometry （FCM） was used tomeasure the protein levels of HIF-1α and p53 and to detect cell apoptosis. The protein levels of HIF-la and p53 were also determined by immunohistochemistry and Western blotting. The expression of HIF-la mRNA was determined by RT-PCR. Results： The protein levels of HIF-1α and p53 were evidently increased in group B compared to group A. The protein levels of HIF-1α and p53 in group C were lower compared to group B; the rate of apoptosis in group C was higher than that in group B. Conclusion： Hypoxia decreased the effect of apoptosis induced by ^60Co γ-ray in Hep-2 human laryngeal cancer cell line. The apoptosis pathway maybe related to some other genes or proteins but not p53 in the conditions of hypoxia and reoxygenation after hypoxia.
