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甲基转移酶DNMT3b和SPG20甲基化在肺腺癌中的相关性研究 被引量:7
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作者 赵宝山 邢志君 +3 位作者 孙光蕊 张志民 张旭 梁宗英 《中国肿瘤临床》 CAS CSCD 北大核心 2021年第19期973-978,共6页
目的:探讨肺腺癌组织中DNA甲基转移酶3b(DNMT3b)表达与SPG20甲基化水平,分析二者相关性及在肺腺癌发病机制中的临床意义。方法:收集2020年4月至2021年4月于承德医学院附属医院收集的70例肺腺癌和癌旁组织,采用焦磷酸测序检测SPG20基因... 目的:探讨肺腺癌组织中DNA甲基转移酶3b(DNMT3b)表达与SPG20甲基化水平,分析二者相关性及在肺腺癌发病机制中的临床意义。方法:收集2020年4月至2021年4月于承德医学院附属医院收集的70例肺腺癌和癌旁组织,采用焦磷酸测序检测SPG20基因甲基化水平,采用实时荧光定量PCR(RT-qPCR)检测SPG20和DNMT3b mRNA的表达,采用免疫组织化学法及Western blot检测SPG20和DNMT3b的蛋白表达。统计分析SPG20甲基化水平与DNMT3b相关性及两者与临床病例特征的相关性。构建DNMT3b下调si-RNA,采用脂质体介导法,将DNMT3b si-RNA、阴性对照si-RNA和空白对照组转染A549细胞。使用RT-qPCR和Wesrern blot检测DNMT3b mRNA和蛋白表达水平,采用焦磷酸测序检测各组SPG20基因甲基化水平。结果:在癌组织和癌旁组织中,SPG20基因甲基化率分别为76.25%±5.74%和13.45%±2.41%,SPG20 mRNA相对表达量为0.18±0.03和1.21±0.17,差异具有统计学意义(均P<0.05)。DNMT3b mRNA相对表达量为1.62±0.27,高于癌旁组织的0.32±0.05;免疫组织化学检测结果显示SPG20蛋白表达阳性率分别为27.14%和72.86%,差异具有统计学意义(P<0.05);DNMT3b蛋白表达阳性率分别为75.71%和22.86%,差异具有统计学意义(P<0.05)。Western blot检测结果显示DNMT3b在癌组织中表达为68.57%±3.18%,高于癌旁组织25.29%±1.35%;SPG20在癌组织中表达为16.87%±1.56%,低于癌旁组织的58.75%±2.68%,差异均具有统计学差异(均P<0.05)。肺腺癌组织中SPG20甲基化和DNMT3b具有相关性(r=0.437,P<0.05);SPG20甲基化和DNMT3b与肿瘤TNM分期、组织分化和淋巴结转移密切相关(P<0.05)。体外实验显示,RNA干扰后DNMT3b si-RNA组中DNMT3b mRNA表达量最低,SPG20基因甲基化率明显下降。结论:在肺腺癌病变过程中,DNMT3b与SPG20基因甲基化存在明显的相关性,DNMT3b高表达可能是SPG20基因甲基化前重要的分子事件,在肺腺癌早期病变诊治中具有潜在应用价值。 展开更多
关键词 甲基转移酶 SPG20基因 甲基化 肺腺癌
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Application and evaluation of Stanghellini model in the determination of crop evapotranspiration in a naturally ventilated greenhouse 被引量:4
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作者 Samuel Joe Acquah Haofang Yan +4 位作者 Chuan Zhang Guoqing Wang baoshan zhao Haimei Wu Hengnian Zhang 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2018年第6期95-103,共9页
Stanghellini model is one of the few models primarily developed to predict the evapotranspiration of crops(ET_(c))in naturally ventilated greenhouses.However,there are insufficient data on the model regarding its use,... Stanghellini model is one of the few models primarily developed to predict the evapotranspiration of crops(ET_(c))in naturally ventilated greenhouses.However,there are insufficient data on the model regarding its use,particularly in China where solar greenhouses without heating systems are fast spreading for vegetable growth and production.The application of Stanghellini model and the evaluation of its performance using meteorological and tomato plant data generated inside an unheated and naturally ventilated multi-span Venlo-type greenhouse is exploited in this study.Model capability was evaluated by utilizing data from sap flow measurements,meteorological and crop data.Measured meteorological data included solar radiation(R_(s)),air temperature(T_(a)),relative humidity(RH)and net radiation(Rn).Average leaf area index(LAI)values measured during the experimental period were 1.00,3.30,4.05 and 2.93;while determined crop coefficients(K_(c))changed from 0.40,0.62,1.12 to 0.83 for the initial stage,development stage,mid-season stage and late-season stage,respectively.Results from the study indicated that the average hourly ET_(c) values of tomato plants using sap flow measurements were 0.165 mm/h,0.148 mm/h,0.192 mm/h and 0.154 mm/h for the initial stage,development stage,mid-season stage and late-season stage,respectively.Meanwhile,the ET_(c) values obtained from calculation using Stanghellini model were 0.158 mm/h,0.152 mm/h,0.202 mm/h and 0.162 mm/h for the initial stage,development stage,mid-season stage and late-season stage,respectively.These ET_(c) values calculated by the Stanghellini model were close to the measured values within the same period.The coefficients of correlation(R^(2))based on hourly ET_(c) for the calibration data was 0.94 and that of the validation dataset was 0.90.Scatter plots of the estimated and measured hourly ET_(c) revealed that the R^(2) and the slope of the regression line for May,June and July were 0.94,0.90,0.96 and 1.15,0.97,1.10 respectively.These data were well represented around the 1:1 regression line.A model sensitivity analysis carried out illustrates how the changes in R_(s) and T_(a) affect greenhouse ET_(c).Stanghellini model was therefore proven to be suitable for ET_(c) estimation with acceptable accuracy in unheated and naturally ventilated greenhouses in the Northeast region of China. 展开更多
关键词 CALIBRATION VERIFICATION crop evapotranspiration naturally ventilated greenhouse sap flow
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