Protective Effects of Mongolian Medicine Borantunggal on Rats with Chronic Renal Insufficiency

[Objectives]To observe the protective effects of Mongolian medicine Borantunggal on rats with chronic renal insufficiency.[Methods]Male Wister rats were gavaged with 50 mg/kg/d adenine for 12 consecutive weeks,and were intervened with positive control drug Niaoduqing and three groups of different doses of Mongolian medicine Borantunggal.The rats general state,such as body posture,hair color,activity status,renal function,thyroid gland,adrenal gland,and gonadal hormone levels,expression levels of inflammatory mediators,α-smooth muscle actin(α-SMA)and nuclear factor-κB(P65)in renal tissue,and histopathological changes of rat kidney were observed before and after treatment.[Results]The recovery of the general state of rats with chronic renal insufficiency in the low dose group after intervention with Mongolian medicine Borantunggal was the most obvious.Compared with the model group,the level of CRE in the low dose Mongolian medicine group decreased,and the difference was statistically significant(P<0.05).Compared with the normal group,the 24-h urinary protein in the low dose Mongolian medicine group had a declining trend,but the difference was not statistically significant(P>0.05).The urea level in low,medium and high dose Mongolian medicine groups showed a declining trend,and the effect of low dose Mongolian medicine was better.Compared with the model group,the feed intake of the low dose Mongolian medicine group had an increasing trend,while P and TCHO also had a declining trend.Compared with the model group,the levels of LH and COR in the low,medium and high dose Mongolian medicine groups decreased significantly,and the difference was statistically significant(P<0.05).The levels of T,T3 and T4 were significantly increased,the difference was statistically significant(P<0.05),and the effect of low dose Mongolian medicine was better.Compared with the model group,the levels of IL-6,TNF-αand HIF-1 in the low,medium and high dose Mongolian medicine groups were significantly reduced,and the difference was statistically significant(P<0.05).The levels of TGF-β1 and Ang II in the low dose Mongolian medicine group were significantly reduced,and the difference was statistically significant(P<0.05).The level of TGF-β1 in the medium and high dose Mongolian medicine groups decreased,and the difference was statistically significant(P<0.05).The expression of NF-κB P65 andα-SMA proteins in the low dose Mongolian medicine group showed a declining trend,which was better than Niaoduqing.[Conclusions]Mongolian medicine Borantunggal has significant protective and preventive effects on rats with adenine-induced chronic renal insufficiency.In particular,the dose of 0.31 kg/d had the most significant protective effect.Its action mechanism may be related to regulating hormone levels in the body,improving renal function,reducing renal inflammatory response,reducing the"three highs"in the kidney,and inhibiting renal tubular epithelial cell-to-mesenchymal transition and deposition of extracellular matrix(ECM).

雌二醇和孕酮对脂多糖诱导的去卵巢小鼠输卵管组织中IL-1βmRNA表达的影响

目的研究雌二醇(E2)和孕酮(P4)对脂多糖(LPS)诱导的去卵巢小鼠急性输卵管炎症组织中白细胞介素-1β(IL-1β)mRNA表达的影响,初步探讨雌、孕激素的抗炎机制。方法将健康雌性KM小鼠随机分为假手术组(LPS+SHAM)、卵巢摘除组(LPS+OVX),卵巢摘除+雌二醇组(LPS+OVX+E2)、卵巢摘除+孕酮组(LPS+OVX+P4)、LPS组及对照组(control),应用HE染色观察各组小鼠输卵管组织病理改变、RT-qPCR法检测各组小鼠输卵管中IL-1βmRNA表达水平。结果观察输卵管组织病理学改变,雌、孕激素可明显减轻LPS所致的输卵管炎症;与对照组比较,LPS组、LPS+SHAM组和LPS+OVX+E2组IL-1βmRNA表达显著下调,LPS+OVX+P4组IL-1βmRNA表达极显著下调;与LPS+OVX组比较,LPS+OVX+E2组IL-1βmRNA表达显著下调,LPS+OVX+P4组IL-1βmRNA表达极显著下调。结论雌、孕激素可抑制小鼠输卵管炎症组织内IL-1βmRNA的表达,从而起到减轻LPS所致的炎症反应的作用。