Accumulating evidence suggests that non-coding RNAs (ncRNAs) are both widespread and functionally important in many eukaryotic organisms. In this study, we employed a special size fractionation and cDNA library cons...Accumulating evidence suggests that non-coding RNAs (ncRNAs) are both widespread and functionally important in many eukaryotic organisms. In this study, we employed a special size fractionation and cDNA library construction method followed by 454 deep sequencing to systematically profile rice intermediate-size ncRNAs. Our analysis resulted in the identification of 1349 ncRNAs in total, including 754 novel ncRNAs of an unknown functional category. Chromosome distribution of all identified ncRNAs showed no strand bias, and displayed a pattern similar to that observed in protein-coding genes with few chromosome dependencies. More than half of the ncRNAs were centered around the plus-strand of the 5' and 3' termini of the coding regions. The majority of the novel ncRNAs were rice specific, while 78% of the small nucleolar RNAs (snoRNAs) were conserved. Tandem duplication drove the expansion of over half of the snoRNA gene families. Furthermore, 90% of the snoRNA candidates were shown to produce small RNAs between 20-30 nt, 80% of which were associated with ARGONAUT proteins generally, and AGOlb in particular. Overall, our findings provide a comprehensive view of an intermediate-size non-coding transcriptome in a monocot species, which will serve as a useful platform for an in-depth analysis of ncRNA functions.展开更多
With the development of computational methods and RNA sequencing technology for assembling the transcriptome, it is becoming clear that the mammal genome is pervasively tran- scribed, and large numbers of long noncodi...With the development of computational methods and RNA sequencing technology for assembling the transcriptome, it is becoming clear that the mammal genome is pervasively tran- scribed, and large numbers of long noncoding RNAs (lncRNAs) composing the major part of the transcriptome have been identified (Ravasi et al., 2006; Birney et al., 2007;展开更多
基金This work was supported by grants from National Basic Research Program of China (973 Program) (2012CB910900) National Natural Science Foundation of China (31171156, U1031001)+1 种基金the Ministry of Science and Technology of China (2011CB100101, 2009DFB30030, 2008AA022301) and the Ministry of Agriculture of China (2008ZX08012-005, 2009ZX08012-021 B).We thank Dr. Ning Wei and Abigail Coplin for reading and commenting this manuscript. No conflict of interest declared.
文摘Accumulating evidence suggests that non-coding RNAs (ncRNAs) are both widespread and functionally important in many eukaryotic organisms. In this study, we employed a special size fractionation and cDNA library construction method followed by 454 deep sequencing to systematically profile rice intermediate-size ncRNAs. Our analysis resulted in the identification of 1349 ncRNAs in total, including 754 novel ncRNAs of an unknown functional category. Chromosome distribution of all identified ncRNAs showed no strand bias, and displayed a pattern similar to that observed in protein-coding genes with few chromosome dependencies. More than half of the ncRNAs were centered around the plus-strand of the 5' and 3' termini of the coding regions. The majority of the novel ncRNAs were rice specific, while 78% of the small nucleolar RNAs (snoRNAs) were conserved. Tandem duplication drove the expansion of over half of the snoRNA gene families. Furthermore, 90% of the snoRNA candidates were shown to produce small RNAs between 20-30 nt, 80% of which were associated with ARGONAUT proteins generally, and AGOlb in particular. Overall, our findings provide a comprehensive view of an intermediate-size non-coding transcriptome in a monocot species, which will serve as a useful platform for an in-depth analysis of ncRNA functions.
基金supported by the grants from the National Natural Science Foundation of China (No. 31300889)
文摘With the development of computational methods and RNA sequencing technology for assembling the transcriptome, it is becoming clear that the mammal genome is pervasively tran- scribed, and large numbers of long noncoding RNAs (lncRNAs) composing the major part of the transcriptome have been identified (Ravasi et al., 2006; Birney et al., 2007;