SWEET transporters are a unique class of sugar transporters that play vital roles in various developmental and physiological processes in plants.While the functions of SWEETs have been well established in model plants...SWEET transporters are a unique class of sugar transporters that play vital roles in various developmental and physiological processes in plants.While the functions of SWEETs have been well established in model plants such as Arabidopsis,their functions in economically important fruit crops like pineapple have not been well studied.Here we aimed to investigate the substrate specificity of pineapple SWEETs by comparing the protein sequences of known glucose and sucrose transporters in Arabidopsis with those in pineapple.Our genome-wide approach and 3D structure comparison showed that the Arabidopsis SWEET8 homolog in pineapple,AcSWEET10,shares similar sequences and protein properties responsible for glucose transport.To determine the functional conservation of AcSWEET10,we tested its ability to complement glucose transport mutants in yeast and analyzed its expression in stamens and impact on the microspore phenotype and seed set in transgenic Arabidopsis.The results showed that AcSWEET10 is functionally equivalent to AtSWEET8 and plays a critical role in regulating microspore formation through the regulation of the Callose synthase5(CalS5),which highlights the importance of SWEET transporters in pineapple.This information could have important implications for improving fruit crop yield and quality by manipulating SWEET transporter activity.展开更多
基金We especially thank Dr Binghua Wu(Fujian Agriculture and Forestry University,China)for kindly providing the yeast mutant strain EBY.VW4000 and Prof.Zhong-Nan Yang(Shanghai Normal University,China)for sharing Atsweet8 seeds.This work was supported by the Science and Technology Major Project of Guangxi(Gui Ke AA22068096)the Science and Technology Innovation Project of Pingtan Science and Technology Research Institute(PT2021007,PT2021003)+2 种基金the General Project of Fujian Natural Science Foundation(2020 J01594)the Project of Guangxi Featured Fruit Innovation Team on Pineapple Breeding and Cultivation Post under the National Modern Agricultural Industry Technology System(nycytxgxcxtd-17-05)the Guangxi Academy of Agricultural Sciences Basic Research Project(Gui Nong Ke 2021YT046).The funding bodies played no role in the design of the study and collection,analysis and interpretation of the data,and writing the manuscript.
文摘SWEET transporters are a unique class of sugar transporters that play vital roles in various developmental and physiological processes in plants.While the functions of SWEETs have been well established in model plants such as Arabidopsis,their functions in economically important fruit crops like pineapple have not been well studied.Here we aimed to investigate the substrate specificity of pineapple SWEETs by comparing the protein sequences of known glucose and sucrose transporters in Arabidopsis with those in pineapple.Our genome-wide approach and 3D structure comparison showed that the Arabidopsis SWEET8 homolog in pineapple,AcSWEET10,shares similar sequences and protein properties responsible for glucose transport.To determine the functional conservation of AcSWEET10,we tested its ability to complement glucose transport mutants in yeast and analyzed its expression in stamens and impact on the microspore phenotype and seed set in transgenic Arabidopsis.The results showed that AcSWEET10 is functionally equivalent to AtSWEET8 and plays a critical role in regulating microspore formation through the regulation of the Callose synthase5(CalS5),which highlights the importance of SWEET transporters in pineapple.This information could have important implications for improving fruit crop yield and quality by manipulating SWEET transporter activity.
