Objective:To investigate the biological effects ofβ-glucans in cell wall of new identified strain Saccharomyces cerevisiae strain YG3-1 on the growth of aquatic invertebrates,in Artemia as model organism.Methods:All ...Objective:To investigate the biological effects ofβ-glucans in cell wall of new identified strain Saccharomyces cerevisiae strain YG3-1 on the growth of aquatic invertebrates,in Artemia as model organism.Methods:All yeasts used in the present study were isolated from Rainbow trout intestine and then cultured in yeast extract-peptone-glycerol medium.Activation of β-glucan in yeasts was performed by chemical treatment with 2-mercaptoethanol(2ME)(3.5%v/v).Then nauplii and larvae individuals of Artemia urmiana and Artemia franciscana(two different species of Artemia as test organisms)were fed with 2ME-treated yeasts during the culture.At the end of experiment,after feeding individual length(total length and growth rate)in adult individuals of Artemia was measured.Results:Following this administration,growth in both species of Artemia was improved(P<0.05).So,the results showed that Artemia urmiana adults individuals that fed with 2MEtreated yeasts had the highest growth and total length.These results were confirmed with growth measurement in adult individuals of Artemia.Conclusions:This study suggested that 2ME-treated Saccharomyces cerevisiae strain YG3-1 yeasts can be used for enhancing the growth of other aquatic invertebrates like shrimps as probiotic supplement and growth promoter.展开更多
基金Supported by Iranian Fisheries Science Research Institute,National Artemia Research Center,Agricultural Research Education and Extension Organization(Grant No.SH/T/93/117).
文摘Objective:To investigate the biological effects ofβ-glucans in cell wall of new identified strain Saccharomyces cerevisiae strain YG3-1 on the growth of aquatic invertebrates,in Artemia as model organism.Methods:All yeasts used in the present study were isolated from Rainbow trout intestine and then cultured in yeast extract-peptone-glycerol medium.Activation of β-glucan in yeasts was performed by chemical treatment with 2-mercaptoethanol(2ME)(3.5%v/v).Then nauplii and larvae individuals of Artemia urmiana and Artemia franciscana(two different species of Artemia as test organisms)were fed with 2ME-treated yeasts during the culture.At the end of experiment,after feeding individual length(total length and growth rate)in adult individuals of Artemia was measured.Results:Following this administration,growth in both species of Artemia was improved(P<0.05).So,the results showed that Artemia urmiana adults individuals that fed with 2MEtreated yeasts had the highest growth and total length.These results were confirmed with growth measurement in adult individuals of Artemia.Conclusions:This study suggested that 2ME-treated Saccharomyces cerevisiae strain YG3-1 yeasts can be used for enhancing the growth of other aquatic invertebrates like shrimps as probiotic supplement and growth promoter.
