To identify the chemical differences which lead to the different therapeutic effects of dried rehmannia root(DRR)and prepared rehmannia root(PRR),we compared the chemical composition of decoctions of randomly purchase...To identify the chemical differences which lead to the different therapeutic effects of dried rehmannia root(DRR)and prepared rehmannia root(PRR),we compared the chemical composition of decoctions of randomly purchased DRR and PRR using ultra performance liquid chromatography(UPLC)coupled with time-of-fight mass spectrometry and high performance liquid chromatography(HPLC)coupled with evaporative light scattering detection(ELSD)with the aid of multivariate statistical analysis.Both approaches clearly revealed compositional and quantitative differences between DRR and PRR.UPLC-MS data indicated stachyose,rehmaiono-side A(or rehmaionoside B),acteoside(or forsythiaside,or isoacteoside),6-O-coumaroylajugol(or 6-O-E-feruloylajugol,or 6-O-Z-feruloylajugol)as important discriminators between DRR and PRR decoctions.HPLC-ELSD analysis showed that the content of fructose in the decoctions of PRR was about four times greater than that of DRR(P<10^(-5)),while sucrose content in the decoctions of PRR was only about one seventh of that in DRR(P<0.01).Our results suggest that some compounds,such as fructose,stachyose and rehmaionoside,may be responsible for the differing therapeutic effects of DRR and PRR.Furthermore,improvements in quality control for PRR,which is currently lacking in the Chinese Pharmacopoeia,are recommended.展开更多
Through anisotropic Ag overgrowth on the surface of Au nanobipyramids(Au NBPs), high-purity and sizecontrolled Ag nanorods(Au/Ag NRs) are obtained by a simplified purification process. The diameters of the Au/Ag NRs a...Through anisotropic Ag overgrowth on the surface of Au nanobipyramids(Au NBPs), high-purity and sizecontrolled Ag nanorods(Au/Ag NRs) are obtained by a simplified purification process. The diameters of the Au/Ag NRs are determined by the size of the as-prepared Au NBPs, and the lengths of the Au/Ag NRs are tunable using different amounts of Ag precursor in the growth solution. Surface-enhanced Raman scattering(SERS) studies using Rhodamine-6G(R6G) as a test molecule indicate that the Au/Ag NRs have excellent sensing potential. The tunable optical properties and strong electromagnetic effect of the Au/Ag NRs, along with their superior SERS signal enhancement, show that Au/Ag NRs are promising for further applications in plasmon sensing and biomolecular detection.展开更多
A novel β-cyclodextrin-poly（2-（2-methoxyethoxy）ethyl methacrylate）-co- poly（ethylene glycol） methacrylate （abbreviated as： β-CD-（P（MEO2MA-co-PEGMA））21） was prepared by using the one-step strategy, and t...A novel β-cyclodextrin-poly（2-（2-methoxyethoxy）ethyl methacrylate）-co- poly（ethylene glycol） methacrylate （abbreviated as： β-CD-（P（MEO2MA-co-PEGMA））21） was prepared by using the one-step strategy, and then the star-shaped copolymers were used in the atom transfer radical polymerization （ATRP）. The structure of star-shaped β- CD-（P（MEO2MA-co-PEGMA））21 copolymers were studied by FTIR, 1H NMR and gel permeation chromatography （GPC）. The star-shaped copolymers could self-assembled into micelles in aqueous solution owing to the outer amphiphilic β-CD as a core and the hydrophilic P（MEO2MA-co-PEGMA） segments as a shell. These thermo-responsive starshaped copolymers micelles exhibited lower critical solution temperature （LCST） in water, which could be finely tuned by changing the feed ratio of MEO2MA to PEGMA. The LCST of star-shaped β-CD-（P（MEO2MA-co-PEGMA））21 copolymer micelles were increased from 35℃ to 58℃ with the increasing content of PEGMA. The results were investigated by DLS and TEM. When the temperature was higher than corresponding LCSTs, the micelles started to associate and form spherical nanoparticles. Therefore, β- CD-（P（MEO2MA-co-PEGMA））21 star-shaped copolymer micelles could be potentially applied in nano-carrier, nano-reactor, smart materials and biomedical fields.展开更多
A novel type of amphiphilic pH-responsive folate-poly（ε-caprolactone）- block-poly（ 2-hydroxyethylmethacrylate ）-co-poly（ 2-（ dimethylamino ）-ethylmethacrylate ） （FA-PCL-b-P（HEMA-co-DMAEMA）） （MFP） block ...A novel type of amphiphilic pH-responsive folate-poly（ε-caprolactone）- block-poly（ 2-hydroxyethylmethacrylate ）-co-poly（ 2-（ dimethylamino ）-ethylmethacrylate ） （FA-PCL-b-P（HEMA-co-DMAEMA）） （MFP） block copolymers were designed and synthe- sized via atom transfer radical polymerization （ATRP） and ring opening polymerization （ROP） techniques. The molecular structures of the copolymers were confirmed with IH NMR, FTIR and GPC measurements. The critical micelle concentration （CMC） of MFP in aqueous solution was extremely low （about 6.54 mglL）. The in vitro release behavior of DOX-Ioaded micelles was significantly accelerated when the pH value of solution decreased from 7.4 to 5.0. In vitro antitumor efficiency was evaluated by incubating DOX- loaded micelles with Hela cells. The results demonstrated that this copolymer possessed excellent biocompatibility, and FA-decorated micelles MFP showed higher cellular uptake than those micelles without the FA moiety, indicating their unique targetability. These folate-conjugated biodegradable micelles are highly promising for targeted cancer chemothe-rapy.展开更多
基金The authors are grateful for financial support from the National Nature Science Foundation of China(Grant Nos.81073161,81130067 and 30730112)the National Basic Research Program of China(Grant No.2011CB505304)+1 种基金the Natural Science Foundation of Beijing(Grant No.7112110)for technical support from Mr.Yong Wang and other technologists of Waters China Ltd.
文摘To identify the chemical differences which lead to the different therapeutic effects of dried rehmannia root(DRR)and prepared rehmannia root(PRR),we compared the chemical composition of decoctions of randomly purchased DRR and PRR using ultra performance liquid chromatography(UPLC)coupled with time-of-fight mass spectrometry and high performance liquid chromatography(HPLC)coupled with evaporative light scattering detection(ELSD)with the aid of multivariate statistical analysis.Both approaches clearly revealed compositional and quantitative differences between DRR and PRR.UPLC-MS data indicated stachyose,rehmaiono-side A(or rehmaionoside B),acteoside(or forsythiaside,or isoacteoside),6-O-coumaroylajugol(or 6-O-E-feruloylajugol,or 6-O-Z-feruloylajugol)as important discriminators between DRR and PRR decoctions.HPLC-ELSD analysis showed that the content of fructose in the decoctions of PRR was about four times greater than that of DRR(P<10^(-5)),while sucrose content in the decoctions of PRR was only about one seventh of that in DRR(P<0.01).Our results suggest that some compounds,such as fructose,stachyose and rehmaionoside,may be responsible for the differing therapeutic effects of DRR and PRR.Furthermore,improvements in quality control for PRR,which is currently lacking in the Chinese Pharmacopoeia,are recommended.
基金National Natural Science Foundation of China(NSFC)(11274173,11374159)Jiangsu Innovation Program for Graduate Education(KYZZ_0091)+1 种基金Scientific Foundation of Nanjing Institute of Technology(CKJB201411,QKJB201409,YKJ201538)Qing Lan Project and Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Through anisotropic Ag overgrowth on the surface of Au nanobipyramids(Au NBPs), high-purity and sizecontrolled Ag nanorods(Au/Ag NRs) are obtained by a simplified purification process. The diameters of the Au/Ag NRs are determined by the size of the as-prepared Au NBPs, and the lengths of the Au/Ag NRs are tunable using different amounts of Ag precursor in the growth solution. Surface-enhanced Raman scattering(SERS) studies using Rhodamine-6G(R6G) as a test molecule indicate that the Au/Ag NRs have excellent sensing potential. The tunable optical properties and strong electromagnetic effect of the Au/Ag NRs, along with their superior SERS signal enhancement, show that Au/Ag NRs are promising for further applications in plasmon sensing and biomolecular detection.
基金Acknowledgements The authors gratefully acknowledge financial supports from the National Natural Science Foundation of China （Grant No. 51662036） and the Bingtuan Innovation Team in Key Areas （2015BD003）.
文摘A novel β-cyclodextrin-poly（2-（2-methoxyethoxy）ethyl methacrylate）-co- poly（ethylene glycol） methacrylate （abbreviated as： β-CD-（P（MEO2MA-co-PEGMA））21） was prepared by using the one-step strategy, and then the star-shaped copolymers were used in the atom transfer radical polymerization （ATRP）. The structure of star-shaped β- CD-（P（MEO2MA-co-PEGMA））21 copolymers were studied by FTIR, 1H NMR and gel permeation chromatography （GPC）. The star-shaped copolymers could self-assembled into micelles in aqueous solution owing to the outer amphiphilic β-CD as a core and the hydrophilic P（MEO2MA-co-PEGMA） segments as a shell. These thermo-responsive starshaped copolymers micelles exhibited lower critical solution temperature （LCST） in water, which could be finely tuned by changing the feed ratio of MEO2MA to PEGMA. The LCST of star-shaped β-CD-（P（MEO2MA-co-PEGMA））21 copolymer micelles were increased from 35℃ to 58℃ with the increasing content of PEGMA. The results were investigated by DLS and TEM. When the temperature was higher than corresponding LCSTs, the micelles started to associate and form spherical nanoparticles. Therefore, β- CD-（P（MEO2MA-co-PEGMA））21 star-shaped copolymer micelles could be potentially applied in nano-carrier, nano-reactor, smart materials and biomedical fields.
基金This work was supported financially by the National Natural Science Foundation of China （Grant Nos. 21367022, 51662036 and 21664013） and the Bingtuan Innovation Team in Key Areas （2015BD003）.
文摘A novel type of amphiphilic pH-responsive folate-poly（ε-caprolactone）- block-poly（ 2-hydroxyethylmethacrylate ）-co-poly（ 2-（ dimethylamino ）-ethylmethacrylate ） （FA-PCL-b-P（HEMA-co-DMAEMA）） （MFP） block copolymers were designed and synthe- sized via atom transfer radical polymerization （ATRP） and ring opening polymerization （ROP） techniques. The molecular structures of the copolymers were confirmed with IH NMR, FTIR and GPC measurements. The critical micelle concentration （CMC） of MFP in aqueous solution was extremely low （about 6.54 mglL）. The in vitro release behavior of DOX-Ioaded micelles was significantly accelerated when the pH value of solution decreased from 7.4 to 5.0. In vitro antitumor efficiency was evaluated by incubating DOX- loaded micelles with Hela cells. The results demonstrated that this copolymer possessed excellent biocompatibility, and FA-decorated micelles MFP showed higher cellular uptake than those micelles without the FA moiety, indicating their unique targetability. These folate-conjugated biodegradable micelles are highly promising for targeted cancer chemothe-rapy.
