Immunotherapy has demonstrated the potential to cure melanoma,while the current response rate is still unsatisfactory in clinics.Extensive evidence indicates the correlation between the eficacy and pre-existing T-cell...Immunotherapy has demonstrated the potential to cure melanoma,while the current response rate is still unsatisfactory in clinics.Extensive evidence indicates the correlation between the eficacy and pre-existing T-cell in tumors,whereas the baseline T-cell infiltration is lacking in low-response melanoma patients.展开更多
The worldwide obesity epidemic has imposed human being a huge burden and attention had been drawn to manipulating the lactic acid bacteria (LAB) to improve obesity symptoms.In this study,a more comprehensive and relia...The worldwide obesity epidemic has imposed human being a huge burden and attention had been drawn to manipulating the lactic acid bacteria (LAB) to improve obesity symptoms.In this study,a more comprehensive and reliable in vitro method was established to screen LAB with greater potential against obesity.Firstly,twenty-eight human feces-derived LAB strains were selected by assessing bile salt-acid tolerance and cholesterol lowering capacity.Subsequently,the triglyceride lowering capacity,bile salt hydrolase (BSH) activity,α-glucosidase activity inhibition capacity and Enterobacter cloacae (E.cloacae) growth inhibition capacity were tested for further evaluating the anti-obesity properties of LAB strains.Principal component analysis (PCA) of these results indicated that Limosilactobacillus reuteri (L.reuteri) NCUH003,Lactiplantibacillus plantarum (L.plantarum) NCUH046 and Limosilactobacillus fermentum (L.fermentum) NCUH068 exhibited favorable anti-obesity properties.Besides,the LAB displayed good gastrointestinal adaptability and antioxidant activity but no hemolytic activity,and they were sensitive to various antibiotics.These results indicated that L.reuteri NCUH003,L.plantarum NCUH046 and L.fermentum NCUH068 are potential for application in obesity treatment.展开更多
基金This work was supported by National Natural Science Foundation of China(Nos.U1903125,31800979)Natural ScienceFoundationof HunanProvinceinChina(2021JJ20084,2021J20092)+6 种基金the Science and Technology Innovation Program of Hunan Province(2021RC3020,2020RC2010)This study was also supported by National Key Research and Development Program of China(2019YFA0111600 and 2019YFE0120800)the Natural Science Foundation of China for Outstanding Young Scholars(82022060)the Youth Talent Program of Hunan Province(2019RS2009)the Natural Science Foundation of Hunan Province for Outstanding Young Scholars(2019JJ30040)Innovation-Driven Project of Central South University(2020CX043)China Postdoctoral Science Foundation Funded Project(2021M693558,2022T150741).
文摘Immunotherapy has demonstrated the potential to cure melanoma,while the current response rate is still unsatisfactory in clinics.Extensive evidence indicates the correlation between the eficacy and pre-existing T-cell in tumors,whereas the baseline T-cell infiltration is lacking in low-response melanoma patients.
基金supported by National Natural Science Foundation of China(Project No.32160547)Central Government Guide Local Special Fund Project(Project No.20212ZDD02008)+2 种基金Vegetable Industry Technology System Post Expert Project of Jiangxi Province(Project No.JXARS-06)State Key Laboratory of Food Science and Technology Nanchang University(Project No.SKLF-ZZA-201910)State Key Laboratory of Food Science and Technology Nanchang University(Project No.SKLF-ZZB-202132).
文摘The worldwide obesity epidemic has imposed human being a huge burden and attention had been drawn to manipulating the lactic acid bacteria (LAB) to improve obesity symptoms.In this study,a more comprehensive and reliable in vitro method was established to screen LAB with greater potential against obesity.Firstly,twenty-eight human feces-derived LAB strains were selected by assessing bile salt-acid tolerance and cholesterol lowering capacity.Subsequently,the triglyceride lowering capacity,bile salt hydrolase (BSH) activity,α-glucosidase activity inhibition capacity and Enterobacter cloacae (E.cloacae) growth inhibition capacity were tested for further evaluating the anti-obesity properties of LAB strains.Principal component analysis (PCA) of these results indicated that Limosilactobacillus reuteri (L.reuteri) NCUH003,Lactiplantibacillus plantarum (L.plantarum) NCUH046 and Limosilactobacillus fermentum (L.fermentum) NCUH068 exhibited favorable anti-obesity properties.Besides,the LAB displayed good gastrointestinal adaptability and antioxidant activity but no hemolytic activity,and they were sensitive to various antibiotics.These results indicated that L.reuteri NCUH003,L.plantarum NCUH046 and L.fermentum NCUH068 are potential for application in obesity treatment.
