Objective:To establish an efficacious and efficient fermentation method of enhancing the anti-adipogenesis effect of mulberry(Morus alba) leaves using Cordyceps militais.Methods:Dried mulberry leaves,dried mulberry le...Objective:To establish an efficacious and efficient fermentation method of enhancing the anti-adipogenesis effect of mulberry(Morus alba) leaves using Cordyceps militais.Methods:Dried mulberry leaves,dried mulberry leaves with50% raw silkworm pupa and raw silkworm pupa were fermented with Cordyceps militais for 4 weeks at 25℃,after which the dried mulberry leaves and fermented product were extracted with 70%ethanol and subjected to high performance liquid chromatography(HPLC).The contents of cordycepin,pelargonidin,chlorogenic acid,iso-quercetin and caffeic acid were determined.We then used the 3 T3-L1 cells to investigate whether extracts of fermentation enhanced anti-adipogenesis activity in vitro.Results:HPLC showed that fermentation changed the contents of cordycepin,pelargonidin,chlorogenic acid,iso-quercetin and caffeic acid.Furthermore,fermented dried mulberry leaves with 50% raw silkworm pupa had a better efficacy of anti-adipogenesis than dried mulberry leaves,fermented dried mulberry leaves and fermented silkworm pupa and inhibited triglycerides accumulation and glucose consumption.Additionally,fermented dried mulberry leaves with50% raw silkworm pupa inhibited PPAR-γ signaling.Conclusions:Fermentation with Cordyceps militaris enhanced antiadipogenesis efficacy of mulberry leaves.展开更多
基金This work was supported by Korea Institute of Planning and Evaluation for Technology in Food,Agriculture,Forestry and Fisheries(IPET)through High Value-added Food Technology Development Program,funded by Ministry of Agriculture,Food and Rural Affairs(MAFRA)(116027032HD020).
文摘Objective:To establish an efficacious and efficient fermentation method of enhancing the anti-adipogenesis effect of mulberry(Morus alba) leaves using Cordyceps militais.Methods:Dried mulberry leaves,dried mulberry leaves with50% raw silkworm pupa and raw silkworm pupa were fermented with Cordyceps militais for 4 weeks at 25℃,after which the dried mulberry leaves and fermented product were extracted with 70%ethanol and subjected to high performance liquid chromatography(HPLC).The contents of cordycepin,pelargonidin,chlorogenic acid,iso-quercetin and caffeic acid were determined.We then used the 3 T3-L1 cells to investigate whether extracts of fermentation enhanced anti-adipogenesis activity in vitro.Results:HPLC showed that fermentation changed the contents of cordycepin,pelargonidin,chlorogenic acid,iso-quercetin and caffeic acid.Furthermore,fermented dried mulberry leaves with 50% raw silkworm pupa had a better efficacy of anti-adipogenesis than dried mulberry leaves,fermented dried mulberry leaves and fermented silkworm pupa and inhibited triglycerides accumulation and glucose consumption.Additionally,fermented dried mulberry leaves with50% raw silkworm pupa inhibited PPAR-γ signaling.Conclusions:Fermentation with Cordyceps militaris enhanced antiadipogenesis efficacy of mulberry leaves.
