Fine Structural Observation on the Oogenesis and Vitellogenesis in Chinese Soft-shelled Turtle, Pelodiseus sinensis

Here we described the ovarian follicle using electron microscopy to examine the vitellogenesis pathway and the special function exerted by follicular cell during growing stage.Through the observation of vitellogenesis under the electron microscope,we found some of the mitochondria(Mt)transform into the yolk granulae gradually.So in this stage,the pathway of vitellolgenesis is

Observations on Localization of Interstitial Cells of Cajal, Nitric Oxide Synthase and Acetylcholinesterase Positive nervesin Goat Oviductal Isthmus and Comparison with Chicken Oviduct

In the goat oviductal isthmus,the ICC showed spindle shaped bipolar morphologies with a large nuclear to cytoplasmic content ratio,numerous mitochondria,rough endoplasmic reticulum and many caveolae.They were frequently found near the nerve fibers and the blood vessels.No specialized contacts could be observed between the ICC and the muscles or nerves,except the labyrinthic

Histochemical Localization of Acetylcholinesterase Positive Nerves in Chicken Oviduct

The motility of the oviduct is not just the cellular interplay,but there are complex underlying phenomenon brought about by the specific molecules,the neurotransmitters that drive this activity in a highly organized manner.Acetylcholine is an important excitatory neurotransmitter that causes the contraction of smooth muscle.We have demonstrated the localization of acetylcholine in

The Expression of c-Kit mRNA in Chicken Oviduct

c-Kit is an oncogene,a member of tyrosine kinase family,it is believed as a specific marker of the interstitial cells of Cajal(ICC).Using the quantitative real-time polymerase chain reaction(RT-qPCR),we have demonstrated the mRNA expression of c-Kit gene in the five segments of the chicken oviduct,the infundibulum,magnum,isthmus,uterus(shell gland),and vagina.The TEM observations

Follicular Atresia in Chinese Soft-shelled Turtle, Pelodiseus Sinensis

In this paper,we described the ovarian histology and characterized the oogenesis and follicular atresia of adult females of Pelodiseus Sinensis in order to compare them with other animals.The ovary of Pelodiseus Sinensis showed a well-developed system of lacunae which was located both in the cortex and the medulla during the oogenesis and atresia.The amorphous substance within the

Examination of ellipsoid high endothelial capillary and lymphocytes migration cytologic base in the spleen of Chinese soft-shelled turtle, Pelodiseus Sinensis.

The architecture feature of spleen white pulp of the Chinese soft-shelled turtle,Pelodiseus Sinensis were examined in detail by the microscopy and electron microscopy.The results showed that the ellipsoid capillary was different from capillary in the other kinds of animals,it belonged to high endothelial capillary.The endothelial cells of the ellipsoid capillary were cube or cylindroid.So,it

Enzyme histochemistry studies on the oviduct of Soft-shelled turtle Trionyx sinensis during breeding season

We used tissue staining method to observe the distribution of alkaline phosphatase(ALP),acid phosphatase(ACP),carbonic anhydrase(CA),ATPase,catalase(CAT)in the oviduct of soft-shelled turtle Trionyx sinensis during the breeding period The results showed that:these enzymes were observed in the oviduct of soft-shelled turtle Trionyx sinensis during breeding period,but the distribution and strength of reactions in each region were different.ALP mainly distributed in the wall of vascular cytoplasm of glands,stronger positive reaction showed in the uterus and the site of sperm storage.ACP mainly distributed in the epithelium and glands.CA appeared in cilia lining epithelium and glands from lamina propria,while the place of sperm storage showed weak response to these areas.CAT showed positive results in glands from different regions,especially,the gland from sperm storage tube(SST).Strong positive reaction of ATPase were observed in epithelial cilia and gland from lamina propria in different regions.These results suggest these enzymes have functions on egg formation,transportation and sperm storage.