Research on the changes and significance of serum RBP4 level in patients with senior coronary heart disease accompanying diabetes mellitus

Objective:To explore the significance of serum Retinol binding protein 4(RBP4)and its relationship with coronary artery lesion in patients with senior coronary heart disease accompanying T2DM by determining the level of serum RBP4 and Gensini score.Methods:30 cases of patients who were 60 years old above with coronary heart disease accompanying T2DM were selected and included in the experimental group,and 30 cases of patients of 60 years old above with coronary heart disease alone were included in the control group.Both groups of patients were given CAG examinations.In addition,Gensini score was calculated according to different degrees and parts of coronary artery lesion.It was required to record each patient’s age,gender,fasting blood glucose(FPG),triglycerides(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),glycated hemoglobin(HbA1c)and other laboratory examination indexes.ELISA was used to detect the level of serum RBP4 in each group,and statistical analysis was performed to the data in each group.Results:(1)RBP4 level,GS score,FPG and LDL-C in the experimental group were all higher than those in the control group,and the difference was of statistical significance(p<.05).There was no statistically significant difference in age,gender,TC,TG and HDL-C between two groups.(2)RBP4 was positively correlated to FPG and HbA1c.(3)In patients with senior coronary heart disease accompanying diabetes mellitus,HbA1c was positively correlated to GS score,RBP4 and FPG.(4)RBP4 was a risk factor for coronary artery stenosis in patients with senior coronary heart disease accompanying diabetes mellitus.Conclusions:The level of serum RBP4 in patients with senior coronary heart disease accompanying diabetes mellitus is higher than that in patients with coronary heart disease alone,with a deeper degree of coronary artery lesion.The level of serum RBP4 is increased with the degree of lesion deepened in patients with senior coronary heart disease,suggesting that the level of serum RBP4 is expected to be an early predictor of coronary artery lesion for patients with senior coronary heart disease.

Four-microRNA signature for detection of type 2 diabetes

BACKGROUND Sensitive,novel,and accurate biomarkers for the detection of physiological changes in type 2 diabetes(T2DM)at an early stage are urgently needed.AIM To build a multi-parameter diagnostic model for the early detection of T2DM.METHODS MiR-148b,miR-223,miR-130a,and miR-19a levels were detected by real-time polymerase chain reaction in serum of healthy controls,individuals with impaired glucose regulation,and T2DM patients.The diagnostic value of miR-148b,miR-223,miR-130a,and miR-19a,alone or in combination,was analyzed.RESULTS The area under the curve(AUC)of miR-223,which had the best diagnostic value for discriminating the impaired glucose regulation and T2DM groups,was 0.84,and the sensitivity and specificity were 73.37%and 81.37%,respectively.The AUC of the four-miRNA signature was 0.90,and the sensitivity and specificity were 78.82%and 88.23%,respectively.In the validation set,the AUC was 0.88,and the sensitivity and specificity were 78.36%and 87.63%,respectively.CONCLUSION In summary,we have built a multi-parameter diagnostic model consisting of miR-148b,miR-223,miR-130a,and miR-19a for the detection of T2DM.It may be a potential tool for the early detection of T2DM.

Relationship of Cystatin-C with coronary artery disease in the elderly patients with diabetes

Objective: To study the relationship between Cystatin C (Cys-C) level and the development of coronary heart disease in elderly patients with type 2 diabetes mellitus (T2DM). Methods: 120 cases of elderly diabetic patients were involved in this study from October 2013 to November 2015 in Depart-ment of Endocrinology and Cardiology. All patients underwent the detection of routine flash dual-source coronary computed tomography angioplasty (CCTA), Cys-C, hemoglobin A1c (HbA1c). According to the difference of CCTA results, the patients were divided into DM1 group (with normal coronary CTA), DM2 group (with a coronary artery stenosis rate < 50%), DM3 group (with a coronary artery stenosis rate of 50%-74%) and DM4 group (with a coronary artery stenosis rate of ≥ 75%). The levels of Cys-C and HbA1c were compared in the four groups. Results: The blood levels of Cys-C and HbA1c showed significant differences between DM1, DM2, DM3 and DM4 group (p <.05). The age, heart rate, blood pressure showed no statistical differences between the groups. The level of Cys-C was positively correlated with HbA1c. Conclusions: The blood level of Cys-C in the elderly patients with T2DM increased with the increase of the degree of coronary artery stenosis. Blood Cys-C may be used as an early predictor of coronary artery lesions in elderly patients with T2DM.

Relationship of plasma homocysteine, soluble intercellular adhesion molecule-1, high mobility group box 1 protein with carotid intima-media thickness in elderly patients with type 2 diabetes mellitus

Objective:To explore the relationship of plasma homocysteine(Hcy),soluble intercellular adhesion molecule-1(sICAM-1)and high mobility group box 1 protein(HMGB1)with carotid intima-media thickness(c-IMT)in elderly patients with type 2 diabetes mellitus.Methods:A total of 100 elderly patients who were diagnosed as type 2 diabetes mellitus in Baogang Hospital of Inner Mongolia from June 2017 to May 2020 were chosen as research objects.According to c-IMT,they were divided into the normal group(n=35),the mild to moderate group(n=41)and the severe group(n=24).The expression levels of plasma Hcy,sICAM-1 and HMGB1 were compared between groups respectively.Pearson’s correlation coefficient was used to analyze the relationship of plasma Hcy,sICAM-1,HMGB1 with c-IMT.Results:The comparison in plasma Hcy,sICAM-1,HMGB1 and c-IMT among the three groups of patients was of statistical significance(p<.05).The results of correlation analysis showed that the expression levels of plasma Hcy,sICAM-1 and HMGB1 were positively correlated with c-IMT in elderly patients with type 2 diabetes mellitus(r=.627,.598,.614;p<.05).Conclusions:The expression levels of plasma Hcy,sICAM-1 and HMGB1 are abnormally increased in elderly patients with type 2 diabetes mellitus,and related to c-IMT,which can provide a strong evidence for clinical diagnosis and treatment by detecting their levels in clinical practice.

吞吐量保持强劲韧性增长 结构持续优化——“十三五”期间沿海港口吞吐量发展特点及未来展望

随着我国经济进入新常态发展,沿海港口吞吐量于"十三五"期间进入了结构调整期。面对复杂多变的国际经贸环境和国内经济下行压力,特别是2018年开始的中美贸易摩擦和2020年全球新冠肺炎疫情等持续冲击,沿海港口在剧烈波动中仍保持了吞吐量持续增长,结构、布局不断优化,港口格局加速调整,显现出内在的强劲韧性。