Objective:The aim of the study was to observe the expressions of genes related to genome stability and DNA repair in the members of nasopharyngeal carcinoma(NPC) clustering families.Methods:In the Zhongshan City where...Objective:The aim of the study was to observe the expressions of genes related to genome stability and DNA repair in the members of nasopharyngeal carcinoma(NPC) clustering families.Methods:In the Zhongshan City where there is highly incidence rate of NPC,we chose the members of the NPC clustering families as objects,and the patients of nasopharyngitis and NPC as the control group.We isolated the RNA from the nasopharyngeal tissue,and synthesized its cRNA,the genome stability and DNA repair genes chip technique,chemiluminescent detection and real-time fluorescence quantitative technique were used to examine the genome stability and DNA repair genes in the nasopharyngeal tissue.Results:More genome stability and DNA repair genes were up-regulated in the members of the NPC clustering families than the NPC patients,and the range of up-regulated was high,with the over up-regulated 100 times genes including TEP1,MSH4,PMS2L1.Fewer genome stability and DNA repair genes were down-regulated in the members of the NPC clustering families than the NPC patients,the ubiquitin genes almost were down-regulated,the results also could be confirmed by real-time fluorescence quantitative PCR.Conclusion:There are specially expression character of genome stability and DNA repair genes in the members of NPC clustering families.展开更多
[Objectives]Through data mining and analysis,the medication characters and ideas of ye Tianshi,a famous physician in Qing Dynasty,in the treatment of tinnitus were explored,so as to provide reference for clinical tinn...[Objectives]Through data mining and analysis,the medication characters and ideas of ye Tianshi,a famous physician in Qing Dynasty,in the treatment of tinnitus were explored,so as to provide reference for clinical tinnitus treatment.[Methods]The medical records of Ye Tianshi's treatment of tinnitus in the Ancient and Modern Consilia Cloud Platform were retrieved and sorted,and the use frequency,properties,metabolic organ and compatibility of related Chinese herbs in the cases were analyzed through system-integrated statistical analysis,cluster analysis,association rules analysis and complex network analysis.[Results]A total of 46 medical cases were finally included,with 15 frequently used Chinese herbs,including poria with hostwood,Radix Rehmanniae Preparata,magnetitum,etc.Most of the drugs are cold and neutral in nature,sweet and bitter in taste,and metabolized in liver and heart.The results of cluster analysis show that the therapeutic drugs for tinnitus are mainly divided into four categories:strengthening spleen,nourishing yin,suppressing symptoms,and clearing heat.The results of association rules analysis show that the commonly used traditional Chinese medicine combination is composed of three herbs,poria with hostwood,Radix Ophiopogonis,and Radix et Rhizoma Glycyrrhizae,which have spleen-strengthening and stomach-tonifying effects.The results of complex network analysis show that the core prescription is based on Siwu Decoction.[Conclusions]In the treatment of tinnitus,Ye Tianshi paid attention to the three internal organs of heart,kidney and gallbladder,and made good use of lung qi-dispersing drugs to clear heat,sweet and cold drugs to tonify stomach and nourish yin,and tonic drugs to suppress symptoms.The core prescription is mainly composed of Radix Rehmanniae,Radix Rehmanniae Preparata,oyster,poria with hostwood,Fructus Forsythiae,Radix Paeoniae Alba,antelope horn,magnetitum and Radix Asparagi.The commonly used single herbs are poria with hostwood,Radix Rehmanniae Preparata and magnetitum.The combination of poria with hostwood,Radix Ophiopogonis and Radix et Rhizoma Glycyrrhizae is more common.展开更多
基金Supported by a grant from the National Natural Science Foundation of China (No.30672713)
文摘Objective:The aim of the study was to observe the expressions of genes related to genome stability and DNA repair in the members of nasopharyngeal carcinoma(NPC) clustering families.Methods:In the Zhongshan City where there is highly incidence rate of NPC,we chose the members of the NPC clustering families as objects,and the patients of nasopharyngitis and NPC as the control group.We isolated the RNA from the nasopharyngeal tissue,and synthesized its cRNA,the genome stability and DNA repair genes chip technique,chemiluminescent detection and real-time fluorescence quantitative technique were used to examine the genome stability and DNA repair genes in the nasopharyngeal tissue.Results:More genome stability and DNA repair genes were up-regulated in the members of the NPC clustering families than the NPC patients,and the range of up-regulated was high,with the over up-regulated 100 times genes including TEP1,MSH4,PMS2L1.Fewer genome stability and DNA repair genes were down-regulated in the members of the NPC clustering families than the NPC patients,the ubiquitin genes almost were down-regulated,the results also could be confirmed by real-time fluorescence quantitative PCR.Conclusion:There are specially expression character of genome stability and DNA repair genes in the members of NPC clustering families.
基金Supported by The Second Batch of Training Projects for Excellent Clinical Talents of Traditional Chinese Medicine in Guangdong Province(Yue Zhong Yi Ban Han[2017]No.267).
文摘[Objectives]Through data mining and analysis,the medication characters and ideas of ye Tianshi,a famous physician in Qing Dynasty,in the treatment of tinnitus were explored,so as to provide reference for clinical tinnitus treatment.[Methods]The medical records of Ye Tianshi's treatment of tinnitus in the Ancient and Modern Consilia Cloud Platform were retrieved and sorted,and the use frequency,properties,metabolic organ and compatibility of related Chinese herbs in the cases were analyzed through system-integrated statistical analysis,cluster analysis,association rules analysis and complex network analysis.[Results]A total of 46 medical cases were finally included,with 15 frequently used Chinese herbs,including poria with hostwood,Radix Rehmanniae Preparata,magnetitum,etc.Most of the drugs are cold and neutral in nature,sweet and bitter in taste,and metabolized in liver and heart.The results of cluster analysis show that the therapeutic drugs for tinnitus are mainly divided into four categories:strengthening spleen,nourishing yin,suppressing symptoms,and clearing heat.The results of association rules analysis show that the commonly used traditional Chinese medicine combination is composed of three herbs,poria with hostwood,Radix Ophiopogonis,and Radix et Rhizoma Glycyrrhizae,which have spleen-strengthening and stomach-tonifying effects.The results of complex network analysis show that the core prescription is based on Siwu Decoction.[Conclusions]In the treatment of tinnitus,Ye Tianshi paid attention to the three internal organs of heart,kidney and gallbladder,and made good use of lung qi-dispersing drugs to clear heat,sweet and cold drugs to tonify stomach and nourish yin,and tonic drugs to suppress symptoms.The core prescription is mainly composed of Radix Rehmanniae,Radix Rehmanniae Preparata,oyster,poria with hostwood,Fructus Forsythiae,Radix Paeoniae Alba,antelope horn,magnetitum and Radix Asparagi.The commonly used single herbs are poria with hostwood,Radix Rehmanniae Preparata and magnetitum.The combination of poria with hostwood,Radix Ophiopogonis and Radix et Rhizoma Glycyrrhizae is more common.
