Mitochondria,double-membrane organelles,are known to participate in a variety of metabolic and signal transduction pathways.The intermembrane space(IMS)of mitochondria is proposed to subject to multiple damages emanat...Mitochondria,double-membrane organelles,are known to participate in a variety of metabolic and signal transduction pathways.The intermembrane space(IMS)of mitochondria is proposed to subject to multiple damages emanating from the respiratory chain.The optic atrophy 1(OPA1),an important protein for mitochondrial fusion,is cleaved into soluble short-form(S-OPA1)under stresses.Here we report that S-OPA1 could function as a molecular chaperone in IMS.We purified the S-OPA1(amino acid sequence after OPA1 isoform 5 S1 site)protein and showed it protected substrate proteins from thermally and chemically induced aggregation and strengthened the thermotolerance of Escherichia coli(E.coli).We also showed that S-OPA1 conferred thermotolerance on IMS proteins,e.g.,neurolysin.The chaperone activity of S-OPA1 may be required for maintaining IMS homeostasis in mitochondria.展开更多
基金the National Key Re-search and Development Program of China(2017YFA0106300)the Stra-tegic Priority Research Program of the Chinese Academy of Sciences(XDA16030505)+6 种基金the National Key Research and Development Program of China(2017YFA0102900,2019YFA09004500,2017YFC1001602,2016YFA0100300,2018YFA0107100)the National Natural Science Foundation of China(32025010,31801168,31900614,31970709,81901275,32070729)the Key Research Program of Frontier Sciences,CAS(QYZDB-SSW-SMC001)International Cooperation Program,CAS(154144KYSB20200006)Guangdong Province Science and Technol-ogy Program(2020B1212060052,2018A030313825,2018GZR110103002,2020A1515011200,2020A1515010919,2020A1515011410,2021A1515012513)Guangzhou Science and Technology Program(201807010067,202002030277,202102021250,202102020827,202102080066)CAS Youth Innovation Promotion Association.
文摘Mitochondria,double-membrane organelles,are known to participate in a variety of metabolic and signal transduction pathways.The intermembrane space(IMS)of mitochondria is proposed to subject to multiple damages emanating from the respiratory chain.The optic atrophy 1(OPA1),an important protein for mitochondrial fusion,is cleaved into soluble short-form(S-OPA1)under stresses.Here we report that S-OPA1 could function as a molecular chaperone in IMS.We purified the S-OPA1(amino acid sequence after OPA1 isoform 5 S1 site)protein and showed it protected substrate proteins from thermally and chemically induced aggregation and strengthened the thermotolerance of Escherichia coli(E.coli).We also showed that S-OPA1 conferred thermotolerance on IMS proteins,e.g.,neurolysin.The chaperone activity of S-OPA1 may be required for maintaining IMS homeostasis in mitochondria.
